Rapid detection of Salmonella sp. in pork samples using fluorescent in situ hybridization: A comparison with VIDAS®-SLM system and ISO 6579 cultural method

Arquivo Brasileiro de Medicina Veterinaria e Zootecnia

Volumn 59, Issue 6, 2007, Pages 1388-1393

  Vieira Pinto, M ^a   Oliveira, M ^b   Bernardo, F ^b   Martins, C ^a  

^a UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

^b FACULDADE DE MEDICINA VETERINÁRIA   (Portugal)

Author keywords

ELFA; FISH; Salmonella; Swine

Indexed keywords

SALMONELLA; SALMONELLA SP.; SUIDAE;

EID: 40349096340     PISSN: 01020935     EISSN: 16784162     Source Type: Journal    
DOI: 10.1590/S0102-09352007000600006     Document Type: Article

References (27)

1. AMANN, R.; LUDWIG, W.; SCHLEIFER, K-H. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev., v.59, p.143-169, 1995.
2. AMANN, R.; FUCHS, B. M.; BEHRENS, S. The identification of microorganisms by fluorescence in situ hybridisation. Cur. Opinion Biotechnol., v.12, p.231-236, 2001
3. AUTY, M.A.E.; GARDINER, G.E.; MCBREARTY, S.J. et al. Direct in situ assessment of bacteria in probiotic dairy products using viability staining in conjunction with confocal scanning laser microscopy. Appl. Environ. Microbiol., v.67, p.420-425, 2001.
4. BLASCO, L.; FERRER, S.; PARDO., I. Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria. FEMS Microbiol. Lett., v.225, p.115-123, 2003.
5. BOTTELDOORN, N.; HEYNDRICKX, M.; RIJPENS, N. et al. Prevalence of Salmonella, Campylobacter and VTEC in pig farm. In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF SALMONELLA AND OTHER FOOD BORNE PATHOGENS IN PORK, 4., 2001, Leipzig. Proceedings... Leipzig, Germany. p. 139-142. (Abstract).
6. CASTAGNA, S.M.F.; SCHARZ, P.; CANAL, C.W. et al. Presença de Salmonella sp. no trato intestinal e em tonsilas/linfonodos submandibulares de suínos ao abate. Arq. Bras. Med. Vet. Zootec., v.56, p.300-306, 2004.
7. CUDJOE, K. S.; KRONA, R. Detection of Salmonella from raw food samples using Dynabeads anti-Salmonella and a conventional reference method. Int. J. Food Microbiol., v.37, p.55-62, 1997.
8. CUI, S.; SCHROEDER, C.M.; ZHANG, D.Y. et al. Rapid sample preparation method for PCR-based detection of Escherichia coli O157:H7 in ground beef. J. Appl. Microbiol., v.95, p.129-134, 2003.
9. D'HAINAUT, L. (Ed.). Conceitos e métodos da estatística. Lisboa: Fundação Calouste Gulbenkian, 1992. 366p.
10. DE LONG, E.F.; WICKHAM, G.S.; PACE, N.R. Phylogenetic stains: ribosomal RNA-based probes for the identification of single microbial cells. Science, v.243, p.1360-1363, 1989.
11. DE MEDICI, D.; PEZZOTTI, G.; MARFOGLIA, D. et al. Comparison between ICS-VIDAS, MSRV and standard cultural method for Salmonella recovery in poultry meat. Int. J. Food Microbiol., v.45, p.205-210, 1998.
12. FANG, Q.; BROCKMANN, S.; BOTZENHART, K. et al. Improved detection of Salmonella sp. in foods by fluorescent in situ hybridization with 23S rRNA probes: a comparison with conventional cultural methods. J. Food Protect., v.66, p.723-731, 2003.
13. GRAY, J.T.; FEDORKA-CRAY, P.J.; STABEL, T.J. et al. Influence of inoculation route on the carrier state of Salmonella choleraesuis in swine. Vet. Microbiol., v.47, p.43-59, 1995.
14. HODSON, R. E.; DUSTMAN, W. A.; GARG, R. P. et al. Prokaryotic in situ PCR: visualization of microscale distribution of specific genes and gene products in prokaryotic communities. Appl. Environ. Microbiol., v.61, p.4074-4082, 1995.
15. JACOB, B.; KORSAK, N.; ETIENNE, G. et al. Serotypes and antimicrobial patterns of Salmonella strains isolated from three different pig production systems: farrow to finish pig herds, weaner to finish pig herds and slaughtered pigs of all origin. In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF SALMONELLA AND OTHER FOOD BORNE PATHOGENS IN PORK, 4., 2001, Leipzig. Proceedings... Leipzig, Germany. p.214-216. (Abstract).
16. JUNG, B.Y.; LEE, W.W.; LEE, H.S. et al. Prevalence of Salmonella in slaughter pigs in South Korea. In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF SALMONELLA AND OTHER FOOD BORNE PATHOGENS IN PORK, 4., 2001, Leipzig. Proceedings... Leipzig, Germany. p.202-204. (Abstract).
17. MORENO, Y.; HERNANDEZ, M.; FERRUS, M.A. et al. Direct detection of thermotolerant campylobacters in chicken products by PCR and in situ hybridization. Res. Microbiol., v.152, p.577-582, 2001.
18. MOTER, A.; GOBEL, U.B. Fluorescence in situ hybridization (FISH) for direct visulaization of microorganisms. J. Microbiol. Methods, v.41, p.85-112, 2000.
19. NORDENTOFT, S.; CHRISTENSEN, H.; WEGENER, H.C. Evaluation of a fluorescence-labelled oligonucleotide probe targeting 23S rRNA for in situ detection of Salmonella serovars in paraffin-embedded tissue sections and their rapid identification in bacterial smears. J. Clin. Microbiol., v.35, p.2642-2648, 1997.
20. OLIVEIRA, M.; BERNARDO, F. Fluorescent In Situ Hybridization aplicado à detecção rápida de Salmonella de origem alimentar e ambiental. Rev. Port. Cien. Vet., v.97, p.81-85, 2002.
21. PLESS, P.; KOFER, J.; FUCHS, K. Salmonella surveillance in Styrian meat cutting plants. In: INTERNATIONAL SYMPOSIUM ON THE EPIDEMIOLOGY AND CONTROL OF SALMONELLA AND OTHER FOOD BORNE PATHOGENS IN PORK, 4., 2001, Leipzig. Proceedings... Leipzig, Germany. p. 36-38. (Abstract).
22. POPOFF, M.Y. (Ed.). Antigenic formulas of the Salmonella serovars. 8.ed. Report of the WHO Collaborating Centre for reference and Research on Salmonella. Paris: Institute Pasteur, 2001.
23. RIJPENS, N.; HERMAN, L.; VEREECKEN, F. et al. Rapid detection of stressed Salmonella sp. in dairy and eggs products using immunomagnetic separation and PCR. Int. J. Food Microbiol., v.46, p.37-44, 1999.
24. SILVA, L.E.; GOTARDI, C.P.; VIZZOTO, J.D. et al. Infecção por Salmonella enterica em suínos criados em um sistema integrado do Sul do Brasil. Arq. Bras. Med. Vet. Zootec., v.58, p.455-461, 2006.
25. VIEIRA-PINTO, M.M.; OLIVEIRA, M.; BERNARDO, F. et al. Evaluation of Fluorescent in situ Hybridization (FISH) as a rapid screening method for detection of Salmonella in tonsils of slaughtered pigs for consumption: a comparison with conventional culture method. J. Food Safety, v.25, p.109-119, 2005.
26. VILLARINO, A.; BOUVET, O.M.M.; REGNAULT, B. et al. Exploring the frontier between life and death in Escherichia coli: evaluation of different viability markers in live and heat- or UV-killed cells. Res. Microbiol., v.151, p.755-768, 2000.
27. YEH, K-S. ; TSAI, C-E. ; CHEN, S-P. et al. Comparison between VIDAS automatic Enzyme-Linked Fluorescent Immunoassay and cultural method for Salmonella recovery from pork carcass sponge samples. J. Food Prot., v.65, p.1656-1659, 2002.