Fate of transgenic plant DNA in the environment

Environmental Biosafety Research

Volumn 6, Issue 1-2, 2007, Pages 15-35

  Pontiroli, Alessandra ^a   Simonet, Pascal ^a   Frostegard, Asa ^b   Vogel, Timothy M ^a   Monier, Jean Michel ^a  

^a ECOLE CENTRALE DE LYON   (France)

^b NORWEGIAN UNIVERSITY OF LIFE SCIENCES   (Norway)

Author keywords

Bacteria; Fate; GMO; Microbial; Transgene

Indexed keywords

PLANT DNA;

BIOLOGICAL MODEL; GENETICS; HORIZONTAL GENE TRANSFER; MICROBIOLOGY; REVIEW; TRANSGENIC PLANT;

DNA, PLANT; GENE TRANSFER, HORIZONTAL; MODELS, BIOLOGICAL; PLANTS, GENETICALLY MODIFIED; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS);

EID: 38949161109     PISSN: 16357922     EISSN: 16357930     Source Type: Journal    
DOI: 10.1051/ebr:2007037     Document Type: Review

References (189)

1. Ahrenholtz I, Lorenz MG, Wackernagel W (1994) The extracellular nuclease of Serratia marcescens: studies on the activity in vitro and effect on transforming DNA in a groundwater aquifer microcosm. Arch. Microbiol. 161: 176-183
2. Altieri MA, Gurr GM, Wratten SD (2004) Genetic engineering and ecological engineering: A clash of paradigms or scope for synergy? In Gurr GM, Wratten SD, Altieri MA, eds, Ecological engineering for pest management: Advances in habitat manipulation for arthropods, Collingwood, Australia: CSIRO Publishing, pp 13-31
3. Ando T, Xu Q, Torres M, Kusugami K, Israel DA, Blaser MJ (2000) Restriction-modification system differences in Helicobacter pylori are a barrier to interstrain plasmid transfer. Mol. Microbiol. 37: 1052-65
4. Angle JS (1994) Release of Transgenic Plants - Biodiversity and Population-Level Considerations. Mol. Ecol. 3: 45-50
5. Aragao FJL, Sarokin L, Vianna GR, Rech EL (2000) Selection of transgenic meristematic cells utilizing a herbicidal molecule results in the recovery of fertile transgenic soybean [Glycine max (L.) Merril] plants at a high frequency. Theoret. Appl. Genet. 101: 1-6
6. Avery OT, MacLeod CM, McCarthy M (1944) Studies on the chemical nature of the substance inducing transformation of pneumococcal types. I. Induction of transformation by a deoxyribonucleic acid fraction isolated from Pneumococcus type III. J. Exp. Med. 79: 137
7. Barry G, Kishore G, Padgette S, Talor M, Kolacz K, Weldon M, Re D, Eichholtz D, Fincher K, Hallas L (1992) Inhibitors of amino acid biosynthesis: strategies for imparting glyphosate tolerance to plants. In Singh BK, Flores HE, Shannon JC, eds, Biosynthesis and Molecular Regulation of Amino Acids in Plants, American Society of Plant Physiology, pp 139-145
8. Baur B, Hanselmann K, Schlimme W, Jenni B (1996) Genetic transformation in freshwater: Escherichia coli is able to develop natural competence. Appl. Environ. Microbiol. 62: 3673-3678
9. Becker R, Ulrich A, Hedtke C, Hornermeier B (2001) Einfluss des Anbaus von transgenem herbizidresistentem Raps auf das Agrarökosystem. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 44: 159-167
10. Belzile FJ (2002) Transgenic, transplastomic and other genetically modified plants: a Canadian perspective. Biochimie 84: 1111-1118
11. Berndt C, Meier P, Wackernagel W (2003) DNA restriction is a barrier to natural transformation in Pseudomonas stutzeri JM300. Microbiol. 149: 895-901
12. Bertolla F, Van Gijsegem F, Nesme X, Simonet P (1997) Conditions for natural transformation of Ralstonia solanacearum. Appl. Environ. Microbiol. 63: 4965-4968
13. Bertolla F, Frostegard A, Brito B, Nesme X, Simonet P (1999) During infection of its host, the plant pathogen Ralstonia solanacearum naturally develops a state of competence and exchanges genetic material. Mol. Plant Microbe Interact. 12: 467-472
14. Bertolla F, Pepin R, Passelegue-Robe E, Paget E, Simkin A, Nesme X, Simonet P (2000) Plant genome complexity may be a factor limiting in situ the transfer of transgenic plant genes to the phytopathogen Ralstonia solanacearum. Appl. Environ. Microbiol. 66: 4161-4167
15. Bickle TA, Kruger DH (1993) Biology of DNA restriction. Microbiol. Rev. 57: 434-450
16. Björklöf K, Suoniemi A, Haahtela K, Romantschuk M (1995) High frequency of conjugation versus plasmid segregation of RP1 in epiphytic Pseudomonas syringae populations. Microbiol. 141: 2719-2727
17. Bolotin A, Wincker P, Mauger S, Jaillon O, Malarme K, Weissenbach J, Ehrlich SD, Sorokin A (2001) The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403. Genome Res. 11: 731-753
18. Boyle JR, Lundkvist H, Smith CT (2001) Ecological considerations for potentially sustainable plantation forests. In Strauss SH, Bradshaw HD, eds, Proc. of the 1st Int. Symp. on Ecological and Societal Aspects of Transgenic Plantations, Stevenson, WA, 22-24 July 2001, College of Forestry, Oregon State University, Corvallis, pp 151-157
19. Bron S, Luxen E, Venema G (1980) Restriction and modification in Bacillus subtilis. Mol. Gen. Genet. 179: 103-110
20. Carrer H, Hockenberry TN, Svab Z, Maliga P (1993) Kanamycin resistance as a selectable marker for plastid transformation in tobacco. Mol. Gen. Genet. 241: 49-56
21. Castaldini M, Turrini A, Sbrana C, Benedetti A, Marchionni M, Mocali S, Fabiani A, Landi S, Santomassimo F, Pietrangeli B, Nuti MP, Miclaus N, Giovannetti M (2005) Impact of Bt corn on rhizospheric and soil eubacterial communities and on beneficial mycorrhizal symbiosis in experimental microcosms. Appl. Environ. Microbiol. 71: 6719-6729
22. Ceccherini MT, Castialdini M, Piovanelli C, Hastings RC, McCarthy AJ, Bazzicalupo M, Miclaus N (1998) Effects of swine manure on autotrophic ammonia-oxidizing bacteria in soil microcosms. Appl. Soil. Ecol. 7: 149-157
23. Ceccherini M, Pote J, Kay E, Van VT, Marechal J, Pietramellara G, Nannipieri P, Vogel TM, Simonet P (2003) Degradation and transformability of DNA from transgenic leaves. Appl. Environ. Microbiol. 69: 673-678
24. Cérémonie H, Buret F, Simonet P, Vogel TM (2004) Isolation of lightning-competent soil bacteria. Appl. Environ. Microbiol. 70: 6342-6346
25. Cérémonie H, Buret F, Simonet P, Vogel TM (2006) Natural electro-transformation of lightning competent Pseudomonas strain in artificial soil microcosms. Appl. Environ. Microbiol. 72: 2385-2389
26. Claverys J-P, Martin B (2003) Bacterial competence genes: signatures of active transformation, or only remnants? Trends Microbiol. 11: 161-165
27. Comai L, Facciotti D, Hiatt WR, Thompson G, Rose RE, Stalker DM (1985) Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741-744
28. Comai L, Larson-Kelly N, Kiser J, Mau CJ, Pokalsky AR, Shewmaker CK, McBride K, Jones A, Stalker DM (1988) Chloroplast transport of a ribulose bisphosphate carboxylase small subunit-5-enolpyruvyl 3-phosphoshikimate synthase chimeric protein requires part of the mature small subunit in addition to the transit peptide. J. Biol. Chem. 263: 15104-15109
29. Crecchio C, Stotzky G (1998) Binding of DNA on humic acids: effect on transformation of Bacillus subtilis and resistance to DNase. Soil Biol. Biochem. 30: 1061-1067
30. Daniell H, Muthukumar B, Lee SB (2001a) Marker free transgenic plants: engineering the chloroplast genome without the use of antibiotic selection. Curr. Genet. 39: 109-116
31. Daniell H, Wiebe PO, Millan AF (2001b) Antibiotic-free chloroplast genetic engineering - an environmentally friendly approach. Trends Plant Sci. 6: 237-239
32. Daniell H, Khan MS, Allison L (2002) Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant. Sci. 7: 84-91
33. Davies J (1994) Inactivation of antibiotics and the dissemination of resistance genes. Science 264: 375-382
34. De Leij F, Sutton E, Whipps J, Fenlon J, Lynch J (1995) Impact of field release of genetically modified Pseudomonas fluorescens on indigenous microbial populations of wheat. Appl. Environ. Microbiol. 61: 3443-3453
35. de Lipthay JR, Barkay T, Sorensen SJ (2001) Enhanced degradation of phenoxyacetic acid in soil by horizontal transfer of the tfdA gene encoding a 2,4-dichlorophenoxyacetic acid dioxygenase. FEMS Microbiol. Ecol. 35: 75-84
36. de Vries J, Wackernagel W (1998) Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation. Mol. Gen. Genet. 257: 606-613
37. de Vries J, Wackernagel W (2002) Integration of foreign DNA during natural transformation of Acinetobacter sp. by homology-facilitated illegitimate recombination. Proc Natl. Acad. Sci. USA 99: 2094-2099
38. de Vries J, Wackernagel W (2004) Microbial horizontal gene transfer and the DNA release from transgenic crop plants. Plant Soil 266: 91-104
39. de Vries J, Meier P, Wackernagel W (2001) The natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in the recipient cells. FEMS Microbiol. Lett. 195: 211-215
40. DeBlock M, Herrera-Estrella L, Van Montagu M, Schell J, Zambryski P (1984) Expression of foreign genes in regenerated plants and in their progeny. EMBO J. 3: 1681-1689
41. DeBlock M, Schell J, Van Montagu M (1985) Chloroplast transformation by Agrobacterium tumefaciens. EMBO J. 4: 1367-1372
42. DeBlock M, De Brower D, Tenning P (1989) Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 91: 694-701
43. Della Cioppa G, Christopher Bauer S, Taylor ML, Rochester DE, Klein BK, Shah DM, Fraley RT, Kishore GM (1987) Targeting a herbicide-resistant enzyme from Escherichia coli to chloroplasts of higher plants. Nat. Biotech. 5: 579-584
44. Demanèche S, Bertolla F, Buret F, Nalin R, Sailland A, Auriol P, Vogel TM, Simonet P (2001a) Laboratory-scale evidence for lightning-mediated gene transfer in soil. Appl. Environ. Microbiol. 67: 3440-3444
45. Demanèche S, Jocteur-Monrozier L, Quiquampoix H, Simonet P (2001b) Evaluation of biological and physical protection against nuclease degradation of clay-bound plasmid DNA. Appl. Environ. Microbiol. 67: 293-299
46. Demanèche S, Kay E, Gourbière F, Simonet P (2001c) Natural transformation of Pseudomonas fluorescens and Agrobacterium tumefaciens in soil. Appl. Environ. Microbiol. 67: 2617-2621
47. Di Giovanni GD, Watrud LS, Seidler RJ, Widmer F (1999) Comparison of parental and transgenic alfalfa rhizosphere bacterial communities using Biolog GN metabolic finger-printing and enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR). Microb. Ecol. 37: 129-139
48. Donegan KK, Seidler RJ, Doyle JD, Porteous LA, Digiovanni G, Widmer F, Watrud LS (1999) A field study with genetically engineered alfalfa inoculated with recombinant Sinorhizobium meliloti: effects on the soil ecosystem. J. Appl. Ecol. 36: 920-936
49. Dubnau D (1999) DNA uptake in bacteria. Annu. Rev. Microbiol. 53: 217-244
50. Dunfield KE, Germida JJ (2001) Diversity of bacterial communities in the rhizosphere and root interior of field-grown genetically modified Brassica napus. FEMS Microbiol. Ecol. 38: 1-9
51. Dunfield KE, Germida JJ (2003) Seasonal changes in the rhizosphere microbial communities associated with field-grown genetically modified canola (Brassica napus). Appl. Environ. Microbiol. 69: 7310-7318
52. Dunfield KE, Germida JJ (2004) Impact of genetically modified crops on soil- and plant-associated microbial communities. J. Environ. Qual. 33: 806-815
53. Dunwell JM (1999) Transgenic Crops: The next generation, or an example of 2020 vision. Annals Botan. 84: 269-277
54. Dykhuizen DE (1998) Santa Rosalia revisited: Why are there so many species of bacteria? Antonie van Leeuwenhoek 73: 25-33
55. Eichholtz DA, Rogers SG, Horsch RB, Klee HJ, Hayford M, Hoffmann NL, Braford SB, Fink C, Flick J, O'Connell KM, Froley RT (1987) Expression of mouse dihydrofolate reductase gene confers methotrexate resistance in transgenic petunia plants. Somatic Cell. Mol. Genet. 13: 67-76
56. Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink CL, Fry JS, Galluppi GR, Goldberg SB, Hoffmann NL, Woo SC (1983) Expression of bacterial genes in plant cells. Proc. Natl. Acad. Sci. USA 80: 4803-4807
57. Franchi M, Bramanti E, Morassi Bonzi L, Luigi Orioli P, Vettori C, Gallori E (1999) Clay-nucleic acid complexes: characteristics and implications for the preservation of genetic material in primeval habitats. Orig. Life Evol. Biosph. 29: 297-315
58. Freyssinet G, Pelissier B, Freyssinet M, Delon R (1996) Crops resistant to oxynils: from the laboratory to the market. Field Crops Res. 45: 125-133
59. Frostegard A, Courtois S, Ramisse V, Clerc S, Bernillon D, Le Gall F, Jeannin P, Nesme X, Simonet P (1999) Quantification of bias related to the extraction of DNA directly from soils. Appl. Environ. Microbiol. 65: 5409-5420
60. Gallori E, Bazzicalupo M, Dal Canto L, Fani R, Nannipieri P, Vettori C, Stotzky G (1994) Transformation of Bacillus subtilis by DNA bound on clay in non-sterile soil. FEMS Microbiol. Ecol. 15: 119-126
61. Gebhard F, Smalla K (1998) Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA. Appl. Environ. Microbiol. 64: 1550-1554
62. Gebhard F, Smalla K (1999) Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer. FEMS Microbiol. Ecol. 28: 261-272
63. Goddijn OJ, van der Duyn Schouten PM, Schilperoort RA, Hoge JH (1993) A chimaeric tryptophan decarboxylase gene as a novel selectable marker in plant cells. Plant. Mol. Biol. 22: 907-912
64. Goldstein DA, Tinland B, Gilbertson LA, Staub JM, Bannon GA, Goodman RE, McCoy RL, Silvanovich A (2005) Human safety and genetically modified plants: a review of antibiotic resistance markers and future transformation selection technologies. J. Appl. Microbiol. 99: 7-23
65. Gossele V, Aarssen R, Cornelissen M (1994) A 6′ gentamicin acetyltransferase gene allows effective selection of tobacco transformants using kanamycin as a substrate. Plant Mol. Biol. 26: 2009-2012
66. Gough KC, Hawes WS, Kilpatrick J, Whitelam GC (2001) Cyanobacterial GR6 glutamate-1-semialdehyde aminotransferase: A novel enzyme-based selectable marker for plant transformation. Plant Cell Rep. 20: 296-300
67. Graham JB, Istock CA (1978) Genetic exchange in Bacillus subtilis in soil. Mol. Genet. Genom. 166: 287-290
68. Graham JB, Istock CA (1979) Gene exchange and natural selection cause Bacillus subtilis to evolve in soil culture. Science 204: 637-639
69. Greaves MP, Wilson MJ (1970) The degradation of nucleic acids and montmorillonite-nucleic-acid complexes by soil microorganisms. Soil Biol. Biochem. 2: 257-268
70. Greenpeace (2003) Monsanto out of our food. Greenpeace International Briefing for the World Social Forum, Porto Alegre, Brazil
71. Griffith F (1928) The significance of pneumococcal types. J. Hyg. 27: 113-159
72. Griffiths B, Caul S, Thompson JA, Birch N, Scrimgeour C, Cortet J, Foggo A, Hackett C, Henning Krogh P (2006) Soil microbial and faunal community responses to Bt maize and insecticide in two soils. J. Environ. Qual. 35: 734-741
73. Guerineau F, Brooks L, Meadows J, Lucy A, Robinson C, Mullineaux P (1990) Sulfonamide resistance gene for plant transformation. Plant Mol. Biol. 15: 127-136
74. Gulden R, Lerat HS, Hart MM, Powell JR, Trevors JT, Pauls PK, Klironomos JN, Swanton CJ (2005) Quantitation of transgenic plant DNA in Leachate water: real-time polymerase chain reaction analysis. J. Agric. Food Chem. 53: 5858-5865
75. Gyamfi S, Pfeifer U, Stierschneider M, Sessitsch A (2002) Effects of transgenic glufosinate-tolerant oilseed rape (Brassica napus) and the associated herbicide application on eubacterial and Pseudomonas communities in the rhizosphere. FEMS Microbiol. Ecol. 41: 181-190
76. Hails R, Kinderlerer J (2003) The GM public debate: context and communication strategies. Nat. Rev. Genet. 4: 819-825
77. Haldrup A, Petersen SG, Okkels FT (1998a) Positive selection: a plant selection principle based on xylose isomerase, an enzyme used in the food industry. Plant Cell Rep. 18: 76-81
78. Haldrup A, Petersen SG, Okkels FT (1998b) The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using D-xylose as the selection agent. Plant Mol. Biol. 37: 287-296
79. Hall L, Topinka K, Huffman J, Davis L, Good A (2000) Pollen flow between herbicide-resistant Brassica napus is the cause of multiple-resistant B. napus volunteers. Weed Sci. 48: 688-694
80. Hastings RC, Ceccherini MT, Miclaus N, Saunders JR, Bazzicalupo M, McCarthy AJ (1997) Direct molecular biological analysis of ammonia oxidising bacteria populations in cultivated soil plots treated with swine manure. FEMS Microbiol. Ecol. 23: 45-54
81. Hayford MB, Medford JI, Hoffman NL, Rogers SG, Klee HJ (1988) Development of a plant transformation selection system based on expression of genes encoding gentamycin acetyltransferases. Plant Physiol. 86: 1216-1222
82. Herrera-Estrella L, De Block M, Messens E, Hernalsteen J-P, Van Montagu M, Schell J (1983) Chimeric genes as dominant selectable markers in plant cells. EMBO J. 2: 987-995
83. Heuer H, Kroppenstedt RM, Lottmann J, Berg G, Smalla K (2002) Effects of T4 lysozyme release from transgenic potato roots on bacterial rhizosphere communities are negligible relative to natural factors. Appl. Environ. Microbiol. 68: 1325-1335
84. Hille J, Verheggen F, Roelvink P, Franssen H, Kammen A, Zabel P (1986) Bleomycin resistance: a new dominant selectable marker for plant cell transformation. Plant. Mol. Biol. 7: 171-176
85. Hiltner L (1904) Über neue Erfahrungen und Probleme auf dem Gebiete der Bodenbakteriologie. Arbeiten der Deutschen Landwirtschaftsgesellschaft 98: 59-78
86. Horsch RB, Fry JE, Hoffmann NL, Eicholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229-1231
87. Howe AR, Gasser CS, Brown SM, Padgette SR, Hart J, Parker GB, Fromm ME, Armstrong CL (2002) Glyphosate as a selective agent for the production of fertile transgenic maize (Zea mays) plants. Mol. Breed. 10: 153-164
88. Irdani T, Bogani P, Mengoni A, Mastromei G, Buiatti M (1998) Construction of a new vector conferring methotrexate resistance in Nicotiana tabacum plants. Plant Mol. Biol. 37: 1079-1084
89. James C (2006) Executive summary of global status of commercialized Biotech/GM Crops: 2006. ISAAA Briefs No. 35, ISAAA: Ithaca, NY
90. Jelenska J, Tietze E, Tempe J, Brevet J (2000) Streptothricin resistance as a novel selectable marker for transgenic plant cells. Plant Cell Rep. 19: 298-303
91. Joersbo M, Okkels FT (1996) A novel principle for selection of transgenic plant cells: positive selection. Plant Cell Rep. 16: 219-221
92. Joersbo M, Donaldson I, Kreiberg J, Peterson SG, Brunstedt J, Okkels FT (1998) Analysis of mannose selection used for transformation of sugar beet. Mol. Breed. 4: 111-117
93. Kaeberlein T, Lewis K, Epstein SS (2002) Isolating "uncultivable" microorganisms in pure culture in a simulated natural environment. Science 296: 1127-1129
94. Kay E, Bertolla F, Vogel TM, Simonet P (2002a) Opportunistic colonization of Ralstonia solanacearum-infected plants by Acinetobacter sp. and its natural competence development. Microbiol. Ecol. 43: 291-297
95. Kay E, Vogel TM, Bertolla F, Nalin R, Simonet P (2002b) In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria. Appl. Environ. Microbiol. 68: 3345-3351
96. Khan SM, Maliga P (1999) Fluorescent antibiotic resistance marker for tracking plastid transformation in higher plants. Nat. Biotechnol. 17: 910-915
97. Khanna M, Stotzky G (1992) Transformation of Bacillus subtilis by DNA bound on montmorillonite and effect of DNase on the transforming ability of bound DNA. Appl. Environ. Microbiol. 58: 1930-1939
98. Kobayashi I (2001) Behavior of restriction-modification systems as selfish mobile elements and their impact on genome evolution. Nucl. Acids Res. 29: 3742-3756
99. Koziel MG, Adams TL, Hazlet MA, Damm D, Miller J, Dahlbeck D, Jayne S, Staskawicz BJ (1984) A cauliflower mosaic virus promoter directs expression of kanamycin resistance in morphogenic transformed plant cells. J. Mol. Appl. Genet. 2: 549-562
100. Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Tiplasmid DNA. Nature 296: 72-74
101. Kunze I, Ebneth M, Heim U, Geiger M, Sonnewald U, Herbers K (2001) 2-Deoxyglucose resistance: A novel selection marker for plant transformation. Mol. Breed. 7: 221-227
102. Lacks SA, Springhorn SS (1984) Transfer of recombinant plasmids containing the gene for DpnII DNA methylase into strains of Streptococcus pneumoniae that produce DpnI or DpnII restriction endonucleases. J. Bacteriol. 158: 905-909
103. Lederberg J, Tatum EL (1946) Gene recombination in Escherichia coli. Nature 158: 558
104. Li YH, Lau PC, Lee JH, Ellen RP, Cvitkovitch DG (2001) Natural genetic transformation of Streptococcus mutans growing in biofilms. J. Bacteriol. 183: 897-908
105. Li YH, Tang N, Aspiras MB, Lau PC, Lee JH, Ellen RP, Cvitkovitch DG (2002) A quorum-sensing signaling system essential for genetic competence in Streptococcus mutans is involved in biofilm formation. J. Bacteriol. 184: 2699-2708
106. Lorenz MG, Sikorski J (2000) The potential for intraspecific horizontal gene exchange by natural genetic transformation: sexual isolation among genomovars of Pseudomonas stutzeri. Microbiol. 146: 3081-3090
107. Lorenz MG, Wackernagel W (1991) High frequency of natural genetic transformation of Pseudomonas stutzeri in soil extract supplemented with a carbon/energy and phosphorus source. Appl. Environ. Microbiol. 57: 1246-1251
108. Lorenz MG, Wackernagel W (1994) Bacterial gene transfer by natural genetic transformation in the environment. Microbiol. Rev. 58: 563-602
109. Lukow T, Dunfield PF, Liesack W (2000) Use of the TRFLP technique to assess spatial and temporal changes in the bacterial community structure within an agricultural soil planted with transgenic and non-transgenic potato plants. FEMS Microbiol. Ecol. 32: 241-247
110. Macneil DJ (1988) Characterization of a unique methyl-specific restriction system in Streptomyces avermitilis. J. Bacteriol. 170: 5607-5612
111. Majewski J, Zawadzki P, Pickerill P, Cohan FM, Dowson CG (2000) Barriers to genetic exchange between bacterial species: Streptococcus pneumoniae transformation. J. Bacteriol. 182: 1016-1023
112. Maliga P, Svab Z, Harper EC, Jones JDG (1988) Improved expression of streptomycin resistance in plants due to a deletion in the streptomycin phosphotransferase coding sequence. Mol. Genet. Genom. 214: 456-459
113. Mansouri H, Petit A, Oger P, Dessaux Y (2002) Engineered rhizosphere: the trophic bias generated by opine-producing plants is independent of the opine type, the soil origin, and the plant species. Appl. Environ. Microbiol. 68: 2562-2566
114. Margulis L (1981) Symbiosis in Cell Evolution. WH Freeman and Company, New York
115. Marvier M (2001) Ecology of transgenic crops. Am. Sci. 89: 160-167
116. Marvier M, vanAcker RC (2005) Can crop transgenes be kept on a leash? Front. Ecol. Environ. 3: 99-106
117. Matic I, Rayssiguier C, Radman M (1995) Interspecies gene exchange in bacteria: The role of SOS and mismatch repair systems in evolution of species. Cell 80: 507-515
118. McFadden GI (2001) Chloroplast origin and integration. Plant Physiol. 125: 50-53
119. Meier P, Wackernagel W (2003a) Mechanisms of homology-facilitated illegitimate recombination for foreign DNA acquisition in transformable Pseudomonas stutzeri. Mol. Microbiol. 48: 1107-1118
120. Meier P, Wackernagel W (2003b) Monitoring the spread of recombinant DNA from field plots with transgenic sugar beet plants by PCR and natural transformation of Pseudomonas stutzeri. Transgenic Res. 12: 293-304
121. Melzak KA, Sherwood CS, Turner RFB, Haynes CA (1996) Driving forces for DNA adsorption to silica in perchlorate solutions. J. Coll. Interface Sci. 181: 635-644
122. Mendum TA, Clark IM, Hirsch PR (2001) Characterization of two novel Rhizobium leguminosarum bacteriophages from a field release site of genetically-modified rhizobia. Antonie van Leeuwenhoek 79: 189-197
123. Miki B, McHugh S (2004) Selectable marker genes in transgenic plants: applications, alternatives and biosafety. J. Biotechnol. 107: 193-232
124. Molin S, Tolker-Nielsen T (2003) Gene transfer occurs with enhanced efficiency in biofilms and induces enhanced stabilisation of the biofilm structure. Curr. Opin. Biotechnol. 14: 255-261
125. Motavalli PP, Kremer RJ, Fang M, Means NE (2004) Impact of genetically modified crops and their management on soil microbially mediated plant nutrient transformations. J. Environ. Qual. 33: 816-824
126. Nakamura Y, Itoh T, Matsuda H, Gobori T (2004) Biased function of horizontally transferred genes in prokaryotic genomes. Nat. Genet. 36: 760-766
127. Nannipieri P, Ascher J, Ceccherini MT, Landi L, Pietramellara G, Renella G (2003) Microbial diversity and soil functions. Europ. J. Soil Sci. 54: 655-670
128. Nielsen KM, Townsend JP (2004) Monitoring and modeling horizontal gene transfer. Nat. Biotechnol. 22: 1110-1114
129. Nielsen KM, van Elsas JD (2001) Stimulatory effects of compounds present in the rhizosphere on natural transformation of Acinetobacter sp. BD413 in soil. Soil Biol. Biochem. 33: 345-357
130. Nielsen KM, van Weerelt MD, Berg TN, Bones AM, Hagler AN, van Elsas JD (1997) Natural transformation and availability of transforming DNA to Acinetobacter calcoaceticus in soil microcosms. Appl. Environ. Microbiol. 63: 1945-1952
131. Nielsen KM, Bones AM, Smalla K, van Elsas JD (1998) Horizontal gene transfer from transgenic plants to terrestrial bacteria: a rare event? FEMS Microbiol. Rev. 22: 99-103
132. Nielsen KM, Smalla K, van Elsas JD (2000a) Natural transformation of Acinetobacter sp. strain BD413 with cell lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in soil microcosms. Appl. Environ. Microbiol. 66: 206-212
133. Nielsen KM, van Elsas JD, Smalla K (2000b) Transformation of Acinetobacter sp. strain BD413 (pFG4?nptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants. Appl. Environ. Microbiol. 66: 1237-1242
134. Normander B, Christensen BB, Molin S, Kroer N (1998) Effect of bacterial distribution and activity on conjugal gene transfer on the phylloplane of the bush bean (Phaseolus vulgaris). Appl. Environ. Microbiol. 64: 1902-1909
135. Oger P, Petit A, Dessaux Y (1997) Genetically engineered plants producing opines alter their biological environment. Nat. Biotechnol. 15: 369-372
136. Oger P, Mansouri H, Dessaux Y (2000) Effect of crop rotation and soil cover on alteration of the soil microflora generated by the culture of transgenic plants producing opines. Mol. Ecol. 9: 881-890
137. Ogram A, Sayler GS, Gustin D, Lewis RJ (1988) DNA Adsorption to soils and sediments. Environ. Sci. Technol. 22: 982-984
138. Olszewski NE, Martin FB, Ausubel FM (1988) Specialized binary vector for plant transformation: expression of the Arabidopsis thaliana AHAS gene in Nicotiana tabacum. Nucleic Acids Res. 16: 10765-10781
139. Otero RDC, Hsieh P (1995) Homologous recombination proteins in prokaryotes and eukaryotes. Annual Rev. Genet. 29: 509-552
140. Ow DW (2002) Recombinase-directed plant transformation for the post-genomic era. Plant. Mol. Biol. 48: 183-200
141. Paget E, Simonet P (1994) On the track of natural transformation in soil. FEMS Microbiol. Ecol. 15: 109-117
142. Paget E, Jocteur Monrozier L, Simonet P (1992) Adsorption of DNA on clay minerals: protection against DNase I and influence on gene transfer. FEMS Microbiol. Lett. 97: 31-40
143. Paget E, Lebrun M, Freyssinet G, Simonet P (1998) The fate of recombinant plant DNA in soil. Europ. J. Soil Biol. 34: 81-88
144. Perez P, Tiraby G, Kallerhoff J, Perret J (1989) Phleomycin resistance as a dominant selectable marker for plant cell transformation. Plant Mol. Biol. 13: 365-373
145. Perl A, Galili S, Shaul O, Ben-Tzvi I, Galili G (1993) Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation. Biotechnol. 11: 715-718
146. Pietramellara G, Dal Canto L, Vettori C, Gallori E, Nannipieri P (1997) Effects of air-drying and wetting cycles on the transforming ability of DNA bound on clay minerals. Soil Biol. Biochem. 29: 55-61
147. Pietramellara G, Franchi M, Gallori E, Nannipieri P (2001) Effect of molecular characteristics of DNA on its adsorption and binding on homoionic montmorillonite and kaolinite. Biol. Fertil. Soils 33: 402-409
148. Poly F, Chenu C, Simonet P, Rouiller J, Jocteur Monrozier L (2000) Differences between linear chromosomal and supercoiled plasmid DNA in their mechanisms and extent of adsorption on clay minerals. Langmuir 16: 1233-1238
149. Poté J, Ceccherini M, Van VT, Rosselli W, Wildi W, Simonet P, Vogel TM (2003) Fate and transport of antibiotic resistance genes in saturated soil columns. Europ. J. Soil Biol. 39: 65-71
150. Prudhomme M, Libante V, Claverys J-P (2002) Homologous recombination at the border: Insertion-deletions and the trapping of foreign DNA in Streptococcus pneumoniae. Proc. Natl. Acad. Sci. USA 99: 2100-2105
151. Quist D (2004) Transgene ecology: An ecological perspective for GMO risk assessment. In Breckling B, Verhoeven R, eds, Risk Hazard Damage - Specification of criteria to assess environmental impact of genetically modified organisms. Bundesamt für Naturschutz. Naturschutz und Biologische Vielfalt, Bonn, pp 239-244
152. Ray JL, Nielsen KM (2005) Experimental methods for assaying natural transformation and inferring horizontal gene transfer. In Zimmer EA, Roalson EH, eds, Methods in Enzymology, Academic Press, pp 491-520
153. Rayssiguier C, Thaler DS, Radman M (1989) The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants. Nature 342: 396-401
154. Redaschi N, Bickle TA (1996) Posttranscriptional regulation of EcoP1I and EcoP15I restriction activity. J. Mol. Biol. 257: 790-803
155. Roberts MS, Cohan FM (1993) The effect of DNA sequence divergence on sexual isolation in Bacillus. Genetics 134: 401-408
156. Romanowski G, Lorenz MG, Wackernagel W (1991) Adsorption of plasmid DNA to mineral surfaces and protection against DNase I. Appl. Environ. Microbiol. 57: 1057-1061
157. Romanowski G, Lorenz MG, Wackernagel W (1993) Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils. Appl. Environ. Microbiol. 59: 3438-3446
158. Saxena D, Stotzky G (2000) Insecticidal toxin from Bacillus thuringiensis is released from roots of transgenic Bt corn in vitro and in situ. FEMS Microbiol. Ecol. 33: 35-39
159. Saxena D, Flores S, Stotzky G (1999) Insecticidal toxin in root exudates from Bt corn. Nature 402: 480
160. Schlüter K, Fütterer J, Potrykus I (1995) Horizontal gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs - if at all - at an extremely low frequency. Biotechnol. 13: 1094-1098
161. Schmalenberger A, Tebbe CC (2002) Bacterial community composition in the rhizosphere of a transgenic, herbicide-resistant maize (Zea mays) and comparison to its nontransgenic cultivar Bosphore. FEMS Microbiol. Ecol. 40: 29-37
162. Sengelov G, Kowalchuk GA, Sørensen SJ (2000) Influence of fungal-bacterial interactions on bacterial conjugation in the residuesphere. FEMS Microbiol. Ecol. 31: 39-45
163. Siciliano SD, Germida JJ (1999) Taxonomic diversity of bacteria associated with the roots of field-grown transgenic Brassica napus cv. Quest, compared to the nontransgenic B. napus cv. Excel and B. rapa cv. Parkland. FEMS Microbiol. Ecol. 29: 263-272
164. Sikorski J, Graupner S, Lorenz M, Wackernagel W (1998) Natural genetic transformation of Pseudomonas stutzeri in a non-sterile soil. Microbiol. 144: 569-576
165. Smiles D (1988) Aspects of the physical environment of soil organisms. Biol. Fertil. Soils 6: 204-215
166. Smith HO, Danner DB, Deich RA (1981) Genetic transformation. Annu. Rev. Biochem. 50: 41-68
167. Smith H, Tomb J, Dougherty B, Fleischmann R, Venter J (1995) Frequency and distribution of DNA uptake signal sequences in the Haemophilus influenzae Rd genome. Science 269: 538-540
168. Spoering AL, Gilmore MS (2006) Quorum sensing and DNA release in bacterial biofilms. Curr. Opin. Microbiol. 9: 133-137
169. Stephens PM, O'Sullivan M, O'Gara F (1987) Effect of bacteriophage on colonization of sugar beet roots by fluorescent Pseudomonas spp. Appl. Environ. Microbiol. 53: 1164-1167
170. Stuy JH (1976) Restriction enzymes do not play a significant role in Haemophilus homospecific or heterospecific transformation. J. Bacteriol. 128: 212-220
171. Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc. Natl. Acad. Sci. USA 90: 913-917
172. Svab Z, Harper EC, Jones JDG, Maliga P (1990) Aminoglycoside- 3′-adenyltransferase confers resistance to spectinomycin and streptomycin in Nicotiana tabacum. Plant Mol. Biol. 14: 197-205
173. Thomas CM, Nielsen KM (2005) Mechanisms of, and barriers to, horizontal gene transfer between bacteria. Nature 3: 711-721
174. Trevors JT (1996) Nucleic acids in the environment. Curr. Opin. Biotechnol. 7: 331-336
175. Ursin VM (1996) Aldehyde dehydrogenase selectable markers for plant transformation. WO 96/12029
176. van Elsas JD, Bailey MJ (2002) The ecology of transfer of mobile genetic elements. FEMS Microbiol. Ecol. 42: 187-197
177. van Elsas JD, Turner S, Bailey MJ (2003) Horizontal gene transfer in the phytosphere. New Phytol. 157: 525-537
178. Vettori C, Paffetti D, Pietramellara G, Stotzky G, Gallori E (1996) Amplification of bacterial DNA bound on clay minerals by the random amplified polymorphic DNA (RAPD) technique. FEMS Microbiol. Ecol. 20: 251-260
179. Waipara NW, Obanor FO, Walter M (2002) Impact of phylloplane management on microbial populations. New Zealand Pl. Prot. 55: 125-128
180. Waldron C, Murphy EB, Roberts JL, Gustafson GD, Armour SL, Malcolm SK (1985) Resistance to hygromycin B. Plant Mol. Biol. 5: 103-108
181. Watrud L, Lee E, Fairbrother A, Burdick C, Reichman J, Bollman M, Storm M, King G, Van de Water P (2004) Evidence for landscape-level, pollen-mediated gene flow from genetically modified creeping bentgrass with CP4 EPSPS as a marker. Proc. Natl. Acad. Sci. USA 101: 14533-14538
182. Weeks JT, Koshiyama KY, Maier-Greiner U, Schaeffner T, Anderson OD (2000) Wheat transformation using cyanamide as a new selective agent. Crop Sci. 40: 1749-1754
183. Widmer F, Seidler RJ, Donegan KK, Reed GL (1997) Quantification of transgenic plant marker gene persistence in the field. Mol. Ecol. 6: 1-7
184. Widmer F, Seidler RJ, Wartrud LS (1996) Sensitive detection of transgenic plant marker gene persistence in soil microcosms. Mol. Ecol. 5: 603-613
185. Wisniewski JP, Frangne N, Massonneau A, Dumas C (2002) Between myth and reality: genetically modified maize, an example of a sizeable scientific controversy. Biochimie 84: 1095-1103
186. Zahrt TC, Maloy S (1997) Barriers to recombination between closely related bacteria: MutS and RecBCD inhibit recombination between Salmonella typhimurium and Salmonella typhi. Proc. Natl. Acad. Sci. USA 94: 9786-9791
187. Zahrt TC, Buchmeier N, Maloy S (1999) Effect of mutS and recD mutations on Salmonella virulence. Infect. Immun. 67: 6168-6172
188. Zhou H, Arrowsmith JW, Fromm ME, Hironaka CM, Taylor ML, Rodriguez D, Pajeau ME, Brown SM, Santino CG, Fry JE (1995) Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Rep. 15: 159-163
189. Zinder N, Lederberg J (1952) Genetic exchange in Salmonella. J. Bacteriol. 64: 679-699