Human safety and genetically modified plants: A review of antibiotic resistance markers and future transformation selection technologies

Journal of Applied Microbiology

Volumn 99, Issue 1, 2005, Pages 7-23

  Goldstein, D A ^a   Tinland, B ^a   Gilbertson, L A ^a   Staub, J M ^a   Bannon, G A ^a   Goodman, R E ^a   McCoy, R L ^a,b   Silvanovich, A ^a  

^a MONSANTO COMPANY   (United States)

^b UNIVERSITY OF NEBRASKA LINCOLN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTICS; GENETIC ENGINEERING;

ANTIBIOTICS RESISTANCE; CURRENT STATUS; GENETICALLY MODIFIED CROPS; HUMAN SAFETY; MARKER GENES; PLANT PRODUCTS; PLANT TRANSFORMATION; PLANT TRANSFORMATION VECTOR; SAFETY INFORMATION; TRANSFORMATION PROCESS;

CROPS;

AMINOGLYCOSIDE ANTIBIOTIC AGENT; AMOXICILLIN PLUS CLAVULANIC ACID; AMPICILLIN; ANTIBIOTIC AGENT; BACTERIAL DNA; BETA LACTAMASE; CRE RECOMBINASE; DNA POLYMERASE; GENE PRODUCT; HYGROMYCIN; KANAMYCIN; NEOMYCIN; PLANT DNA; SPECTINOMYCIN; STREPTOMYCIN; VIRUS DNA;

BIOTECHNOLOGY; GENETICALLY MODIFIED ORGANISM; MICROBIOLOGY; SAFETY;

ACINETOBACTER; ANTIBIOTIC RESISTANCE; BACTERIAL FLORA; BACTERIUM; BIOTECHNOLOGICAL PROCEDURES; DIETARY INTAKE; DNA MODIFICATION; FECES ANALYSIS; GENE TRANSFER; GENETIC RECOMBINATION; GENETICALLY MODIFIED CROP; HOMOLOGOUS RECOMBINATION; HORIZONTAL GENE TRANSFER; HUMAN; ILEOSTOMY; MULTIDRUG RESISTANCE; NONHUMAN; PLANT; PLANT BIOTECHNOLOGY; REVIEW; SAFETY; SMALL INTESTINE; SOYBEAN; TRANSGENIC PLANT;

EID: 21244461143     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2005.02595.x     Document Type: Review

References (136)

1. Alangaden, G.J., Kreiswirth, B.N., Aouad, A., Khetarpal, M., Igno, F.R., Moghazeh, S.L., Manavathu, E.K. and Lerner, S.A. (1998) Mechanism of resistance to amikacin and kanamycin in Mycobacterium tuberculosis. Antimicrob Agents Chemother 42, 1295-1297.
2. Andersen, J.T., Schafer, T., Jorgensen, P.L. and Moller, S. (2001) Using inactivated microbial biomass as fertilizer: the fate of antibiotic resistance genes in the environment. Res Microbiol 152, 823-833.
3. Aragao, F.J.L., Barros, L.M.G., Brasileiro, A.C.M., Ribeiro, S.G., Smith, F.D., Sanford, J.C., Faria, J.C. and Rech, E.L. (1996) Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment. Theor Appl Genet 93, 142-150.
4. Armstrong, J.L., Shigeno, D.S., Calomiris, J.J. and Seidler, R.J. (1981) Antibiotic-resistant bacteria in drinking water. Appl Environ Microbiol 42, 277-283.
5. Atkinson, B. (1986) Species incidence and trends of susceptibility to antibiotics in the United States and other countries: MIC and MBC. In Antibiotics in Laboratory Medicine ed. Lorian, V. pp. 995-1162. Baltimore, MD: Williams and Wilkins.
6. Aumaitre, A., Aulrich, K., Chesson, A., Flachowsky, G. and Piva, G. (2002) New feeds from genetically modified plants: substantial equivalence, nutritional equivalence, digestibility, and safety for animals and the food chain. Livest Prod Sci 74, 223-238.
7. Azucena, E. and Mobashery, S. (2001) Aminoglycoside-modifying enzymes: mechanisms of catalytic processes and inhibition. Drug Resist Updat 4, 106-117.
8. Badosa, E., Moreno, C. and Montesinos, E. (2004) Lack of detection of ampicillin resistance gene transfer from Bt176 transgenic corn to culturable bacteria under field conditions. FEMS Microbiol Ecol 48, 169-178.
9. Barro, F., Rooke, L., Bekes, F., Gras, P., Tatham, A.S., Fido, R., Lazzeri, P.A., Shewry, P.R. et al. (1997) Transformation of wheat with high molecular weight subunit genes results in improved functional properties. Nat Biotechnol 15, 1295-1299.
10. Bartlet, J.G. (1997) Pocket Book of Infectious Disease Therapy. Baltimore, MD: Williams and Wilkins.
11. Bastian, I., Rigouts, L., Palomino, J.C. and Portaels, F. (2001) Kanamycin susceptibility testing of Mycobacterium tuberculosis using mycobacterium growth inhibitor tube and colorometric method. Antimicrob Agents Chemother 45, 1934-1936.
12. Beever, D.E. and Kemp, C.F. (2000) Safety issues associated with the DNA in animal feed derived from genetically modified crops. A review of scientific and regulatory procedures. Nutr Abst Rev Ser B Livest Feeds Feed 70, 175-182.
13. Bertolla, F. and Simonet, P. (1999) Horizontal gene transfers in the environment: natural transformation as a putative process for gene transfers between transgenic plants and microorganisms. Res Microbiol 150, 375-384.
14. Bertolla, F., Gijsegem, F.V., Nesme, X. and Simonet, P. (1997) Conditions for natural transformation of Ralstonia solanacearum. Appl Environ Microbiol 63, 4965-4968.
15. Boshoff, H.I.M., Reed, M.B., Barry, C.E. and Mizrah, V. (2003) DnaE2 polymerase contributes to the in vivo survival and the emergence of drug resistance in Mycobacterium tuberculosis. Cell 113, 183-193.
16. Bunny, K.L., Hall, R.M. and Stokes, H.W. (1995) New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301. Antimicrob Agents Chemother 39, 686-693.
17. Calgene, Inc. (1993) Food Additive Petition for the APH(3′) II as a Processing Aid. FDA Docket Number: 93F-0232. Davis, CA: Calgene, Inc.
18. Calomiris, J.J., Armstrong, J.L. and Seidler, R.J. (1984) Association of metal tolerance with multiple antibiotic resistance of bacteria isolated from drinking water. Appl Environ Microbiol 47, 1238-1242.
19. Chambers, P.A., Duggan, P.S., Heritage, J. and Forbes, J.M. (2001) The fate of antibiotic resistance marker genes in transgenic plant feed material fed to chickens. J Antimicrob Chemother 47, 241-243.
20. Chiew, Y.F., Yeo, S.F., Hall, L.M.C. and Livermore, D.M. (1998) Can susceptibility to an antimicrobial be restored by halting its use? The case of streptomycin versus Enterobacteriaceae. J Antimicrob Chemother 41, 247-252.
21. Chowgule, R.V. and Deodhar, L. (1998) Pattern of secondary acquired drug resistance to antituberculosis drug in Mumbai, India - 1991-1995. Indian J Chest Dis Allied Sci 40, 23-31.
22. Christou, P. and Swain, W.F. (1990) Cotransformation frequencies of foreign genes in soybean cell cultures. Theor Appl Genet 79, 337-341.
23. Christou, P., Swain, W.F., Yang, N.S. and McCabe, B. (1989) Inheritance and expression of foreign genes in transgenic soybean plants. Proc Natl Acad Sci USA 86, 7500-7504.
24. Corpet, D.E. (1988) Antibiotic resistance from food. N Engl J Med 318, 1206-1207.
25. Courvalin, P. (1996) Evasion of antibiotic action by bacteria. J Antimicrob Chemother 37, 855-869.
26. Courvalin, P. (1998) Plantes transgeniques et antibiotiques. Recherche 309, 36-40.
27. Dale, E.G. and Ow, D.W. (1990) Intra- and intermolecular site-specific recombination in plant cells mediated by bacteriophage P1 recombinase. Gene 91, 79-85.
28. Dale, E.G. and Ow, D.W. (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc Natl Acad Sci USA 88, 10558-10562.
29. Daley, M., Knauf, V.C., Summerfelt, K.R: and Turner, J.C. (1998) Co-transformation with one Agrobacterium tumefaciens strain containing two binary plasmids as a method for producing marker-free transgenic plants. Plant Cell Rep 17, 489-496.
30. Damm, B., Schmidt, R. and Willmitzer, L. (1989) Efficient transformation of Arabidopsis thaliana using direct gene transfer to protoplasts. Mol Gen Genet 217, 6-12.
31. DANMAP Report (1997) Consumption of Antimicrobial Agents and Occurrence of Antimicrobial Resistance in Bacteria from Food Animals, Food and Humans in Denmark. Copenhagen, Denmark: Danish Zoonosis Centre. ISSN 1397-078X.
32. Davison, J. (1999) Genetic exchange between bacteria in the environment. Plasmid 42, 73-91.
33. Day, A. (2003) Antibiotic resistance genes in transgenic plants: their origins, undesirability and technologies for their elimination from genetically modified crops. In Transgenic Plants: Current Innovations and Future Trends ed. Stewart, N.C. pp. 111-156. Wymondham, UK: Horizon Scientific Press.
34. De Neve, M., De Buck, S., Jacobs, A., Van Montagu, M. and Depicker, A. (1997) T-DNA integration patterns in co-transformed plant cells suggest that T-DNA repeats originate from co-integration of separate T-DNAs. Plant J 11, 15-29.
35. DeBlock, M. and Debrouwer, D. (1991) Two T-DNA's co-transformed into Brassica napus by a double Agrobacterium tumefaciens infection are mainly integrated at the same locus. Theor Appl Genet 82, 257-263.
36. DeFramond, A.J., Back, E.W., Chilton, W.S., Kayes, L. and Chilton, M.D. (1986) Two unlinked T-DNAs can transform the same tobacco plant cell and segregate in the F1 generation. Mol Gen Genet 202, 125-131.
37. Depicker, A., Herman, L., Jacobs, A., Schell, J. and van Montagu, M. (1985) Mol Gen Genet 201, 477-484.
38. Donnelly, J.P. (1993) Selective decontamination of the digestive tract and its role in antimicrobial prophylaxis. J Antimicrob Chemother 31, 813-829.
39. Duggan, P.S., Chambers, P.A., Heritage, J. and Forbes, M. (2000) Survival of free DNA encoding antibiotic resistance from transgenic maize and the transformation activity of DNA in ovine saliva, ovine rumen fluid, and silage effluent. FEMS Microbiol Lett 191, 71-77.
40. Ebinuma, H., Sugita, K., Matsunaga, E. and Yamakado, M. (1997) Selection of marker-free transgenic plants using the isopentenyl transferase gene. Proc Natl Acad Sci U S A 94, 2117-2121.
41. European Federation of Biotechnology (2001) Task group on public perceptions of biotechnology. Antibiotic Resistance Markers in Genetically Modified (GM) Crops. Briefing paper 10. Delft, the Netherlands: European Federation of Biotechnology.
42. Flavell, R.B., Dart, E., Fuchs, R.L. and Fraley, R.T. (1992) Selectable marker genes: safe for plants? Bio/Technology 10, 141-144.
43. Friedberg, E.C. and Fischhaber, P.L. (2003) TB or not TB: how Mycobacterium tuberculosis may evade drug treatment. Cell 113, 139-140.
44. Fuchs, R.L., Ream, J.E., Hammond, B.G., Naylor, M.W., Leimgruber, R.M. and Berberich, S.A. (1993) Safety assessment of the neomycin phosphotransferase II (npt-II) protein. Bio/Technology 11, 1543-1547.
45. Gebhard, F. and Smalla, K. (1998) Transformation of Acinetobacter sp. strain BD413 by transgenic sugarbeet DNA. Appl Environ Microbiol 64, 1550-1554.
46. Gebhard, F. and Smalla, K. (1999) Monitoring field releases of genetically modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer. FEMS Microbiol Ecol 28, 261-272.
47. Gilbert, G.S., Parke, J.L., Clayton, M.K. and Handelsman, J. (1993) Effects of introduced bacterium on bacterial communities on roots. Ecology 74, 840-854.
48. Gilbertson, L. (2003) Cre-lox recombination: creative tools for plant biotechnology. Trends Biotechnol 21, 550-555.
49. Gillespie, S.H. (2002) Evolution of drug resistance in Mycobacterium tuberculosis: clinical and molecular perspective. Antimicrob Agents Chemother 46, 267-274.
50. Gilman, A.B., Goodman, L.S., Rall, T.W. and Murad, F.ed. (1996) Goodman and Gilman's: the Pharmacological Basis of Therapeutics, 9th edn. New York: Macmillan Publishing Company, Inc.
51. Gleave, A.P., Mitra, D.S., Mudge, S.R. and Morris, B.A. (1999) Selectable marker-free transgenic plants without sexual crossing: transient expression of ere recombinase and use of a conditional lethal dominant gene. Plant Mol Biol 40, 223-235.
52. Goto, F., Toki, S. and Uchimya, H. (1993) Inheritance of a co-transferred foreign gene in the progenies of transgenic rice plants. Transgenic Res 2, 300-305.
53. Haldrup, A., Petersen, S.G. and Okkels, F.T. (1998) The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using D-xylose as the selection agent. Plant Mol Biol 37, 287-296.
54. Hefron, F., Sublett, R., Hedges, R.W. Jacob, A. and Falkow, S. (1975) Origin of the TEM beta-lactamase gene found on plasmids. J Bacteriol 122, 250-256.
55. Herve, C., Rouan, D., Guerche, P., Montane, M.H. and Yot, P. (1993) Molecular analysis of transgenic rapeseed plants obtained by direct transfer of two separate plasmids containing, respectively, the cauliflower mosaic virus coat protein gene and a selectable marker gene. Plant Sci 91, 181-193.
56. Hood, E.E. (2003) Selecting the fruits of your labors. Trends Plant Sci 8, 357-358.
57. Jelenic, S. (2003) Controversy associated with the common component of most transgenic plants kanamycin resistance marker gene. Food Technol Biotechnol 41, 183-190.
58. Jiang, S.C. and Paul, J.H. (1998) Gene transfer by transduction in the marine environment. Appl Environ Microbiol 64, 2780-2787.
59. Joersbo, M. and Okkels, F.T. (1996) A novel principle for selection of transgenic plant cells: positive selection. Plant Cell Rep 16, 219-221.
60. Joersbo, M., Donaldson, I., Kreiberg, J., Petersen, S. et al. (1998) Analysis of mannose selection used for transformation of sugar beet. Mol Breed 4, 111-117.
61. Jonas, D.A., Elmadfa, I., Engle, K.H., Heller, K.J., Kozianowski, G., Konig, A., Muller, D., Narbonne, J.F. et al. (2001) Safety considerations of DNA in food. Ann Nutr Metab 45, 235-254.
62. Kalpana, G.V., Bloom, B.R. and Jacobs, W.R. (1991) Insertional mutagenesis and illegitimate recombination in mycobacteria. Proc Natl Acad Sci U S A 88, 54333-54337.
63. Kay, E., Vogel, T.M., Bertolla, F., Nalin, R. and Simonet, P. (2002) In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria. Appl Environ Microbiol 68, 3345-3351.
64. Kelch, W.J. and Lee, J.S. (1978) Antibiotic resistance patterns of Gram-negative bacteria isolated from environmental sources. Appl Environ Microbiol 36, 450-456.
65. Kilby, N.J., Davies, G.J., Snaith, M.R. and Murray, A.H. (1995) Plant J 8, 637-652.
66. Komari, T., Hiei, Y., Saito, Y., Murai, N. et al. (1996) Vectors carrying two separate T-DNAs for cotransformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J 10, 165-174.
67. Lesmana, M., Leron, C.I., Taslim, D., Tjaniadi, P., Subekti, D., Wasfy, M.O., Campbell, J.R. and Oyofo, B.A. (2001) In vitro antibiotic susceptibility of Neisseria gonorrhoeae in Jakarta, Indonesia. Antimicrob Agents Chemother 45, 359-362.
68. Levy, S.B. (1978) Emergence of antibiotic-resistant bacteria in the intestinal flora of farm inhabitants. J Infect Dis 137, 688-690.
69. Levy, S.B., Marshall, B., Schluederberg, S., Rouse, D. et al. (1988) High frequency of antimicrobial resistance in human fecal flora. Antimicrob Agents Chemother 32, 1801-1806.
70. Libiakova, G., Jorgenson, B., Palmgren, G., Ulvskov, P. and Johansen, E. (2001) Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformations. Plant Cell Rep 20, 610-615.
71. Lichtenstein, C., Paszkowski, J. and Hohn, B. (1994) Intrachromosomal recombination between genomic repeats. In Homologous Recombination and Gene Silencing in Plants ed. Paszkowski, J. pp. 95-122. Dordrecht, the Netherlands: Kluwer.
72. Lloyd, A.M. and Davis, R.W. (1994) Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum. Mol Genet Genom 242, 653-657.
73. Lyznik, L.A., Ryan, R.D., Ritchie, S.W. and Hodges, T.K. (1989) Stable co-transformation of maize protoplasts with gusA and neo genes. Plant Mol Biol 13, 151-161.
74. Lyznik, L.A., Mitchell, J.C., Hirayama, L. and Hodges, T.K. (1993) Activity of yeast FLP recombinase in maize and rice protoplasts. Nucleic Acids Res 21, 969-975.
75. Maeser, S. and Kahmann, R. (1991) The Gin recombinase of phage Mu can catalyse site-specific recombination in plant protoplasts. Mol Gen Genet 230, 170-176.
76. Malik, V.S. and Saroha, M.K. (1999) Marker gene controversy in transgenic plants. J Plant Biochem Biotechnol 8, 1-13.
77. Martin-Orue, S.M., O'Donnel, A.G., Arino, J., Netherwood, T., Gilbert, H.J. and Mathers, J.C. (2002) Degradation of transgenic DNA from genetically modified soya and maize in human intestinal simulations. Br J Nutr 87, 533-542.
78. Reynolds, J.E.F. (1996) Martindale, The Extra Pharmacopeia, 31st edn. pp. 275-276. London: Royal Pharmaceutical Society.
79. Mathur, M.L., Khatri, P.K. and Base, C.S. (2000) Drug resistance in tuberculosis patients in Jodhpur district. Indian J Med Sci 54, 55-58.
80. McKnight, T.D., Lillis, M.T. and Simpson, R.B. (1987) Segregation of genes transferred to one plant cell from two separate Agrobacterium strains. Plant Mol Biol 8, 439-445.
81. Medeiros, A.A. and Crellen, J. (2000) Evaluation of the Sirscan automated zone reader in a clinical microbiology laboratory. J Clin Microbiol 38, 1688-1693.
82. Mercer, D.K., Scott, K.P., Bruce-Johnson, W.A., Glover, L.A. et al. (1999) Fate of free DNA and transformation of the oral bacterium Streptococcus gordonii DL1 by plasmid DNA in human saliva. Appl Environ Microbiol 65, 6-10.
83. Mercer, D.K., Scott, K., Melville, C.M., Glover, L.A. and Flint, H.J. (2001) Transformation of an oral bacterium via chromosomal integration of free DNA in the presence of human saliva. FEMS Microbiol Lett 200, 163-167.
84. Miki, B. and McHugh, S. (2004) Selectable marker genes in transgenic plants: applications, alternatives, and biosafety. J Biotechnol 107, 193-232.
85. Netherwood, T., Martin-Orue, S.M., O'Donnell, A.G., Gockling, S., Graham, J., Mathers, J.C. and Gilbert, H.J. (2004) Assessing the survival of transgenic plant DNA in the human gastrointestinal tract. Nat Biotechnol 22, 204-209.
86. Neu, H.C. (1992) The crisis in antibiotic resistance. Science 257, 1064-1073.
87. Nielsen, K.M., Gebhard, F., Smalla, K., Bones, A.M. et al. (1997a) Evaluation of possible horizontal gene transfer from transgenic plants to the soil bacterium Acinetobacter calcoaceticus BD413. Theor Appl Genet 95, 815-821.
88. Nielsen, K.M., van Weerelt, M., Berg, T.N., Bones, A.M., Hagler, A.N. and van Elsas, J.D. (1997b) Natural transformation and availability of transforming DNA to Acinetobacter calcoaceticus in soil microorganisms. Appl Environ Microbiol 63, 1945-1952.
89. Nielsen, K.M., Smalla, K. and van Elsas, J.D. (2000a) Natural transformation of Acinetobacter sp. Strain BD413 with cell lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in soil microcosms. Appl Environ Microbiol 66, 206-212.
90. Nielsen, K.M., van Elsas, J.D. and Smalla, K. (2000b) Transformation of Acinetobacter sp. strain BD413(pFG4?npt-II) with transgenic plant DNA in soil microcosm and effects of kanamycin on selection of transformants. Appl Environ Microbiol 66, 1237-1242.
91. Nwosu, V.C. (2001) Antibiotic resistance with particular reference to soil microorganisms. Res Microbiol 152, 421-430.
92. Nwosu, V.C. and Ladapo, J.A. (1999) Antibiotic response and plasmid profile of bacteria isolated from a landfill. Curr Microbiol 39, 249-253.
93. Odell, J., Caimi, P., Sauer, B. and Russell, S. (1990) Site-directed recombination in the genome of transgenic tobacco. Mol Gen Genet 223, 369-378.
94. Okkels, F.T., Ward, J.L. and Joersbo, M. (1997) Synthesis of cytokinin glucuronides for the selection of transgenic plant cells. Phytochemistry 46, 801-804.
95. Onouchi, H., Yokoi, K., Machida, C., Matsuzaki, H., Oshima, Y., Matsuoka, K., Nakamura, K. and Machida, Y. (1991) Operation of an efficient site-specific recombination system of Zygosaccharomyces rouxii in tobacco cells. Nucleic Acids Res 19, 6373-6378.
96. Pablos-Mendez, A., Raviglione, M.C., Laszlo, A., Binkin, N., Rieder, H.L., Bustreo, F., Cohn, D.L., Lambregts-van Weezenbeek, C.S. et al. (1998) Global surveillance for antituberculosis-drug resistance, 1994-1997. N Engl J Med 338, 1641-1649.
97. Padgette, S.R., Kolacz, K.H., Delannay, D.B., Re, B.J., LaValle, C.N., Tinius, W.K., Rhodes, Y.I., Otero, G.F. et al. (1995) Development, identification, and characterization of a glyphosate-tolerant soybean line. Crop Sci 35, 1451-1461.
98. Paget, E., Lebrun, M., Freyssinet, G. and Simonet, P. (1998) The fate of recombinant plant DNA in soil. Eur J Soil Biol 34, 81-88.
99. Peerbolte, R., Krens, F.A., Mans, R.M.W., Floor, M., Hoge, J.H.C., Wullems, G.J. and Schilperoort, R.A. (1985) Transformation of plant protoplasts with DNA: co-transformation of non-selected calf thymus carrier DNA and meiotic segregation of transforming DNA sequences. Plant Mol Biol 5, 235-246.
100. Potera, C. (1998) New method churns out TB mutants. Science 280, 1350-1351.
101. Prakash, C.S. (2002) Tuberculosis and antibiotic resistance. Curr Sci 82, 17-18.
102. Ramaswamy, S. and Musser, J.M. (1998) Molecular genetic basis of antimicrobial agent resistance in Mycobacterium tuberculosis: 1998 update. Tuberc Lung Dis 79, 3-29.
103. Redenbaugh, K., Hiatt, W., Martineau, B., Lindeman, J. et al. (1994) Aminoglycoside 3′-phosphotransferase II (APH(3′)II): review of its safety and use in the production of genetically engineered plants. Food Biotechnol 8, 137-165.
104. Reiss, B., Klemm, M., Kosak, H. and Schell, J. (1996) RecA protein stimulates homologous recombination in plants. Proc Natl Acad Sci USA 93, 3094-3098.
105. Russell, S.H., Hoopes, J.L. and Odell, J.T. (1992) Directed excision of a transgene from the plant genome. Mol Gen Genet 234, 49-59.
106. Sande, M.A., Kapusnik-Uner, J.E. and Mandell, G.L. (1990) Antimicrobial agents - general considerations. In The Pharmacological Basis of Therapeutics ed. Gillman, A.G., Rail, T.W., Nies, A.S. and Taylor, P. pp. 1018-1046. New York: Pergammon.
107. Schaffner, W. (1980) Direct transfer of cloned genes from bacteria to mammalian cells. Proc Natl Acad Sci USA 77, 2163-2167.
108. Schluter, K., Futterer, J. and Potrykus, I. (1995) Horizontal gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs - if at all - at an extremely low frequency. Bio/Technology 13, 1094-1098.
109. Schmitz, F.J., Fluit, A.C., Gondolf, M., Beyrau, R., Lindenlauf, E., Verhoef, J., Heinz, H.P. and Jones, M.E. (1999) The prevalence of aminoglycoside resistance and corresponding resistance genes in clinical isolates of staphylococci from 19 European hospitals. J Antimicrob Chemother 43, 253-259.
110. Schubbert, R., Lettmann, C. and Doerfler, W. (1994) Ingested foreign (phage M13) DNA survives transiently in the gastrointestinal tract and enters the bloodstream of mice. Mol Gen Genet 242, 495-504.
111. Schubbert, R., Renz, B., Schmitz, B. and Doerfler, W. (1997) Foreign (M13) DNA ingested by mice reaches peripheral leukocytes, spleen, and liver via the intestinal wall mucosa and can be covalently linked to mouse DNA. Proc Natl Acad Sci USA 94, 961-966.
112. Shaw, K.J., Rather, P.N., Hare, R.S. and Miller, G.H. (1993) Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes. Microbiol Rev 57, 138-163.
113. Silva, P.E.A., Bigi, F., de la Paz Santangelo, A., Romano, M.I., Martin, C., Cataldi, A. and Ainsa, J.A. (2001) Characterization of P55, a multidrug efflux pump in Mycobacterium bovis and Mycobacterium tuberculosis. Antimicrob Agents Chemother 45, 800-804.
114. Smalla, K., Heuer, H., Gotz, A., Niemeyer, D., Krogerrecklenfort, E. and Tietze, E. (2000) Exogenous isolation of antibiotic resistance plasmids from piggery manure slurries reveals a high prevalence and diversity f Inc-Q-like plasmids. Appl Environ Microbiol 66, 4854-4862.
115. Smith, C.A. and Baker, E.N. (2002) Aminoglycoside antibiotic resistance by enzymatic deactivation. Curr Drug Targets Infect Disord 2, 143-160.
116. Society of Toxicology (2002) Society of Toxicology Position Paper - the Safety of Foods Produced Through Biotechnology [WWW document]. URL http://www.toxicology.org/information/governmentmedia/gm_food.doc [accessed on 1 March 2005).
117. Sonti, R.V., Tissier, A.F., Wong, D., Viret, J.F. and Signer, E.R. (1995) Activity of the yeast FLP recombinase in Arabidopsis. Plant Mol Biol 28, 1127-1132.
118. Spencer, T.M., Gordon-Kamm, W.J., Daines, R.J., Start, W.G. and Lemaux, P.G. (1990) Bialaphos selection of stable transformants from maize cell culture. Theor Appl Genet 79, 625-631.
119. Sudupak, M.A., Bennetzen, J.L. and Hulbert, S.H. (1993) Unequal exchange and meiotic instability of disease-resistance genes in the Rp1 region of maize. Genetics 133, 119-125.
120. Sugita, K., Kasahara, T., Matsunaga, E. and Ebinuma, H. (2000) A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J 22, 461-469.
121. Sykes, R.B. and Matthew, M. (1976) The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics. Antimicrob Chemother 2, 115-157.
122. Tepfer, D., Garcia-Gonzales, R., Mansouri, H., Seruga, M., Message, B., Leach, F. and Perica, M.C. (2003) Homology-dependant DNA transfer from plants to soil bacterium under laboratory conditions: implications in evolution and horizontal gene transfer. Transgenic Res 12, 425-437.
123. The Royal Society of London (2000) Transgenic Plants and World Agriculture. pp. 15-18. Washington, DC: National Academy Press.
124. Thomson, J.A. (2001) Horizontal gene transfer of DNA from GM crops to bacteria and to mammalian cells. Concise Rev Hypothesis Food Sci 66, 188-193.
125. Varaiya, A. and Gogate, A. (1998) Drug resistance to the first line of antitubercular regimen (a preliminary report). Indian J Public Health 42, 126-130.
126. deVries, J. and Wackernagel, W. (1998) Detection of npt-II (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation. Mol Gen Genet 257, 606-613.
127. deVries, J., Meier, P. and Wackernagel, W. (2001) The natural transformation of the soil bacterium Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in the recipient cells. FEMS Microbiol Lett 195, 211-215.
128. Wan, Y. and Lemaux, P.G. (1994) Generation of large numbers of independently transformed fertile barley plants. Plant Physiol 104, 37-48.
129. WHO (1991) Management of Patients with Sexually Transmitted Diseases. World Health Organisation Technical Report Series 810. pp. 85. Geneva: World Health Organisation.
130. WHO (2000) Safety Aspects of Genetically Modified Foods of Plant Origin. Report of a joint FAO/WHO expert consultation on foods derived from biotechnology. Geneva: World Health Organization.
131. Wright, G.D. and Thompson, P.R. (1999) Aminoglycoside phosphotransferases: proteins, structure, and mechanism. Front Biosci 4, d9-d21.
132. Wu, X., Zhang, J., Zhuang, Y., Zhang, X., Li, G. and He, X. (1999) Molecular mechanisms of drug resistance in Mycobacterium tuberculosis clinical isolates. China Med J (English) 112, 524-528.
133. Yamamoto, T., Yamagata, S., Horii, K. and Yamagishi S. (1982) Comparison of transcription of beta-lactamase genes specified by various ampicillin transposons. J Bacteriol 150, 269-276.
134. Zhang, W., Subbarao, S., Addae, P., Shen, A., Armstrong, C., Peschke, V. and Gilbertson, L. (2003) Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Theor Appl Genet 107, 1157-1168.
135. Zhong, H., Sum, B., Warkentin, D., Zhang, S., Wu, R., Wu, T. and Sticklen, M. (1996) The competence of maize shoot meristems for integrative transformation and inherited expression of transgenes. Plant Physiol 110, 1097-1107.
136. Zubko, E., Scutt, C. and Meyer, P. (2000) Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat Biotechnol 18, 442-445.