Quantification of transgenic plant marker gene persistence in the field

Molecular Ecology

Volumn 6, Issue 1, 1997, Pages 1-7

  Widmer, F ^a   Seidler, R J ^a   Donegan, K K ^b   Reed, G L ^c  

^a U S ENVIRONMENTAL PROTECTION AGENCY   (United States)

^b DYNAMAC CORPORATION   (United States)

^c OREGON STATE UNIVERSITY   (United States)

Author keywords

biotechnology; DNA persistence; environmental DNA preparation; field experiment; quantitative PCR; risk assessment

Indexed keywords

BRASSICA OLERACEA VAR. BOTRYTIS; CAULIFLOWER MOSAIC VIRUS; NICOTIANA TABACUM; SOLANUM TUBEROSUM;

EID: 0031046816     PISSN: 09621083     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-294X.1997.00145.x     Document Type: Article

References (33)

1. An G, Watson BD, Stachel S, Gordon MP, Nester EW (1985) New cloning vehicles for transformation of higher plants. EMBO Journal, 4, 277-284.
2. Beck E, Ludwig G, Auerswald EA, Reiss B, Schaller H (1982) Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene, 19, 327-336.
3. Becker J, Siegert H, Logemann J, Schell J (1994) Begleitende Sicherheitsforschung zur Freisetzung genetisch veränderter Petunien. In: Biologische Sicherheit. Forschung - Biotechnologie, pp. 563-578. Bundesministerium für Forschung und Technologie, Bonn, Germany.
4. Blake NK, Ditterline RL, Stout RG (1991) Polymerase chain reaction used for monitoring multiple gene integration in Agrobacterium-mediated transformation. Crop Science, 31, 1686-1688.
5. Carlson TA, Chelm BK (1986) Apparent eukaryotic origin of glutamine synthase II from the bacterium Bradyrhizobium japonicum. Nature, 322, 568-570.
6. Depicker A, Stachel S, Dhaese P, Zambryski P, Goodman HM (1982) Nopaline Synthase: transcript mapping and DNA sequence. Journal of Molecular and Applied Genetics, 1, 561-573.
7. Dietz A (1993) Risk assessment of genetically modified plants introduced into the environment. In: Transgenic Organisms, Risk Assessment of Deliberate Release (ed. Wöhrmann K, Tomiuk J), pp. 209-227. Birkhäuser Verlag, Basel, Switzerland.
8. Donegan KK, Schaller DL, Stone JK et al. (1995) Microbial populations, fungal species diversity, and plant pathogen levels in field plots of potato plants expressing the Bacillus thuringiensis var. tenebrionis endotoxin. Transgenic Research, 5, 25-35.
9. Donegan KK, Seidler RJ, Fieland VJ et al. (1996) Decomposition of genetically engineered tobacco under field conditions: persistence of the proteinase inhibitor I product and effects on soil microbial respiration and protozoa, nematode, and microarthropod populations. Journal of Applied Ecology, in press.
10. Doolittle RF, Feng DF, Anderson KL, Alberro MR (1990) A naturally occurring horizontal gene transfer from a eukaryote to a prokaryote. Journal of Molecular Evolution, 31, 383-388.
11. Flavell RB, Dart E, Fuchs RL, Fraley RT (1992) Selectable marker genes: safe for plants? Bio/Technology, 10, 141-144.
12. Froman BE, Tait RC, Gottlieb LD (1989) Isolation and characterization of the phosphoglucose isomerase gene from Escherichia coll Molecular and General Genetics, 217, 126-131.
13. Gardner RC, Hoswarth AJ, Hahn P et al. (1981) The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M13mp7 shotgun cloning. Nucleic Acids Research, 9, 2871-2888.
14. Graham JS, Pearce G, Merryweather J et al. (1985) Wound-induced proteinase inhibitors from tomato leaves. Journal of Biological Chemistry, 260, 6555-6560.
15. Greaves MP, Wilson MJ (1970) The degradation of nucleic acids and montmorillonite- nucleic-acid complexes by soil microorganisms. Soil Biology and Biochemistry, 2, 257-268.
16. Johnson R, Narváez J, Gynheung A, Ryan CA (1989) Expression of proteinase inhibitors I and II in transgenic tobacco plants: effects on natural defense against Manduca sexta larvae. Proceeding of the National Academy of Sciences of the USA, 86, 9871-9875.
17. Khanna M, Stotzky G (1992) Transformation of Bacillus subtilis by DNA bound on montmorillonite and effects of DNase on the transforming ability of bound DNA. Applied and Environmental Microbiology, 58, 1930-1939.
18. Lorenz MG, Wackernagel W (1987) Adsorption of DNA to sand and variable degradation rates of adsorbed DNA. Applied and Environmental Microbiology, 53, 2948-2952.
19. Lorenz MG, Wackernagel W (1994) Bacterial gene transfer by natural genetic transformation in the environment. Microbiological Reviews, 58, 563-602.
20. Molecular Ecology (1994) Symposium Issue: Ecological Implications of Transgenic Plant Release. Molecular Ecology, 3, 1-89.
21. Narváez-Vásquez J, Orozco-Cardénas ML, Ryan CA (1992) Differential expression of a chimeric CaMV-tomato proteinase inhibitor gene in leaves of transformed nightshade, tobacco and alfalfa plants. Plant Molecular Biology, 20, 1149-1157.
22. Ogram AV, Mathot ML, Harsh JB, Boyle J, Pettigrew CJrA (1994) Effects of DNA polymer length on its adsorption to soil. Applied and Environmental Microbiology, 60, 393-396.
23. Perlak FJ, Stone TB, Muskopf YM et al (1993) Genetically improved potatoes: protection from damage by Colorado potato beetles. Plant Molecular Biology, 22, 313-321.
24. Porteous LA, Armstrong JL, Seidler RJ, Watrud LS (1994) An effective method to extract DNA from environmental samples for polymerase chain reaction amplification and DNA fingerprint analysis. Current Microbiology, 29, 301-307.
25. Prins TW, Zadoks JC (1994) Horizontal gene transfer in plants, a biohazard? Outcome of a literature review. Euphytica, 76, 133-138.
26. Reed GL, Jensen C, Hamm C et al. (1995) Integrated pest management of potato insect pests and vectored diseases. 1994-1995 Research Progress Reports, pp. 61-79 Oregon Potato Commission.
27. Rissler J, Mellon M (1993) Perils Amidst the Promise. Ecological Risks of Transgenic Crops in a Global Market. Union of Concerned Scientists, Cambridge, MA, USA.
28. Romanowski G, Lorenz MG, Wackernagel W (1993) Use of polymerase chain reaction and electroporation of Escherichia coli to monitor the persistence of extracellular plasmid DNA introduced into natural soils. Applied and Environmental Microbiology, 59, 3438-3446.
29. Sanders PR, Winter JA, Barnason AR, Rogers SG, Fraley RT (1987) Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants. Nucleic Acids Research, 15, 1543-1558.
30. Schlüter K, Fütterer J, Potrykus I (1995) Horizontal gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs - if at all - at an extremely low frequency. Bio/Technology, 13, 1094-1098.
31. Siegert H, Schell J, Logemann J (1992) Analysis of natural gene transfer mechanisms between transgenic plants and microorganisms under field test conditions. In: Proceedings of the 2nd International Symposium on the Biosafety Results of Field Tests of Genetically Modified Plants and Microorganisms (ed. Casper R, Landsmann J), pp. 273. Biologische Bundesanstalt für Land- und Forstwirtschaft, Braunschweig, Germany.
32. Smalla K, Gebhard F, van Elsas JD, Matzk A, Schiemann J (1994) Bacterial communities influenced by transgenic plants. In: Proceedings of the 3rd International Symposium on the Biosafety Results of Field Tests of Genetically Modified Plants and Microorganisms (ed. Jones DD), pp. 157-166. University of California, Oakland, CA, USA, Monterey, CA, USA.
33. Widmer F, Seidler RJ, Watrud LS (1996) Sensitive detection of transgenic plant marker gene persistence in soil microcosms. Molecular Ecology, 5, 603-613.