Characterization of the substrate mimic bound to engineered prostacyclin synthase in solution using high-resolution NMR spectroscopy and mutagenesis: Implication of the molecular mechanism in biosynthesis of prostacyclin

Biochemistry

Volumn 47, Issue 2, 2008, Pages 680-688

  Ruan, Ke He ^a   Wu, Jiaxin ^a   Cervantes, Vanessa ^a  

^a UNIVERSITY OF HOUSTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; MOLECULAR MECHANICS; MUTAGENESIS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEINS;

PROSTACYCLIN SYNTHASE; SUBSTRATE MIMIC BOUND;

SUBSTRATES;

15 HYDROXY 11ALPHA,9ALPHA EPOXYMETHANOPROSTA 5,13 DIENOIC ACID; PROSTACYCLIN; PROSTACYCLIN SYNTHASE; PROSTAGLANDIN H2; TRYPTOPHAN;

ARTICLE; BINDING SITE; BIOSYNTHESIS; CONCENTRATION RESPONSE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME CONFORMATION; ENZYME ENGINEERING; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; ISOMERIZATION; MUTAGENESIS; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; TITRIMETRY;

15-HYDROXY-11 ALPHA,9 ALPHA-(EPOXYMETHANO)PROSTA-5,13-DIENOIC ACID; CRYSTALLOGRAPHY, X-RAY; CYTOCHROME P-450 ENZYME SYSTEM; EPOPROSTENOL; HUMANS; INTRAMOLECULAR OXIDOREDUCTASES; MAGNETIC RESONANCE SPECTROSCOPY; MOLECULAR CONFORMATION; MOLECULAR MIMICRY; MUTAGENESIS; MUTATION; PROSTAGLANDIN H2; PROTEIN ENGINEERING; PROTONS; SOLUBILITY; SOLUTIONS; SUBSTRATE SPECIFICITY; TITRIMETRY; TRYPTOPHAN;

EID: 38849099652     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi701671q     Document Type: Article

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