Purification of FLAG-Tagged Eukaryotic Initiation Factor 2B Complexes, Subcomplexes, and Fragments from Saccharomyces cerevisiae

Methods in Enzymology

Volumn 431, Issue , 2007, Pages 1-13

  Mohammad Qureshi, Sarah S ^a   Haddad, Raphaël ^a   Palmer, Karren S ^a   Richardson, Jonathan P ^a   Gomez, Edith ^a   Pavitt, Graham D ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BUFFER; GUANINE NUCLEOTIDE EXCHANGE FACTOR; PLASMID VECTOR; FLAG PEPTIDE; HYBRID PROTEIN; INITIATION FACTOR 2; MULTIPROTEIN COMPLEX; PEPTIDE; PEPTIDE FRAGMENT; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; CELL GROWTH; CYTOLYSIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; REVIEW; SACCHAROMYCES CEREVISIAE; BIOLOGICAL MODEL; CELL PROLIFERATION; CHEMISTRY; CULTURE TECHNIQUE; DIALYSIS; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; PHYSIOLOGY; PLASMID;

EUKARYOTA; SACCHAROMYCES CEREVISIAE;

CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CHROMATOGRAPHY, AFFINITY; DIALYSIS; EUKARYOTIC INITIATION FACTOR-2; GENE EXPRESSION; GENETIC VECTORS; MODELS, BIOLOGICAL; MULTIPROTEIN COMPLEXES; PEPTIDE FRAGMENTS; PEPTIDES; PLASMIDS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 38449120943     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(07)31001-X     Document Type: Review

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