Flow cytometry for high-throughput, high-content screening

Current Opinion in Chemical Biology

Volumn 8, Issue 4, 2004, Pages 392-398

  Edwards, Bruce S ^a   Oprea, Tudor ^a   Prossnitz, Eric R ^a   Sklar, Larry A ^a  

^a UNIVERSITY OF NEW MEXICO   (United States)

Author keywords

2 adrenergic receptor; 2AR; formyl peptide receptor; FPR; G protein coupled receptor; GFP; GPCR; green fluorescent protein; high throughput; HT; virtual screening; VS

Indexed keywords

FLUORESCENT DYE;

ANALYTIC METHOD; ASSAY; CELL SCREENING; CELL SUSPENSION; DRUG RESEARCH; FLOW CYTOMETRY; FLUORESCENCE; QUANTITATIVE ANALYSIS; REVIEW; SIGNAL TRANSDUCTION;

AUTOMATION; DRUG DESIGN; FLOW CYTOMETRY; HUMANS; LIGANDS; RECEPTORS, CELL SURFACE; TIME FACTORS;

EID: 3342898403     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpa.2004.06.007     Document Type: Review

References (25)

1. Sklar L.A., Edwards B.S., Graves S.W., Nolan J.P., Prossnitz E.R. Flow cytometric analysis of ligand-receptor interactions and molecular assemblies. Annu Rev Biophys Biomol Struct. 31:2002;97-119
2. De Rosa S.C., Brenchley J.M., Roederer M. Beyond six colors: a new era in flow cytometry. Nat Med. 9:2003;112-117 The authors review the recent development of specialized flow cytometry systems and methodologies for making ultra-high content biological measurements. Immunology applications are emphasized.
3. Nolan J.P., Sklar L.A. Suspension array technology: evolution of the flat-array paradigm. Trends Biotechnol. 20:2002;9-12 This a brief review of recent advances in the rapidly expanding technology area of multiplex bead-based assay systems for flow cytometry.
4. Kettman J.R., Davies T., Chandler D., Oliver K.G., Fulton R.J. Classification and properties of 64 multiplexed microsphere sets. Cytometry. 33:1998;234-243
5. Edwards B.S., Kuckuck F., Sklar L.A. Plug flow cytometry: An automated coupling device for rapid sequential flow cytometric sample analysis. Cytometry. 37:1999;156-159
6. Edwards B.S., Kuckuck F.W., Prossnitz E.R., Ransom J.T., Sklar L.A. HTPS flow cytometry: a novel platform for automated high throughput drug discovery and characterization. J Biomol Screen. 6:2001;83-90
7. Edwards B.S., Kuckuck F.W., Prossnitz E.R., Okun A., Ransom J.T., Sklar L.A. Plug flow cytometry extends analytical capabilities in cell adhesion and receptor pharmacology. Cytometry. 43:2001;211-216
8. Edwards BS, Sklar LA: Plug flow cytometry. Current Protocols Cytometry 2001:1.17.11-11.17.10.
9. Kuckuck F.W., Edwards B.S., Sklar L.A. High throughput flow cytometry. Cytometry. 44:2001;83-90
10. ® system to a range of cell and bead-based endpoint measurements. A novel method is described for maintaining cells and particles in suspension during pre-analysis assay manipulations.
11. Buranda T., Lopez G., Simons P., Pastuszyn A., Sklar L. Detection of epitope-tagged proteins in flow cytometry: Fluorescence resonance energy transfer-based assays on beads with femtomole resolution. Anal Biochem. 298:2001;151-162
12. Jackson W.C., Kuckuck F., Edwards B.S., Mammoli A., Gallegos C.M., Lopez G.P., Buranda T., Sklar L.A. Mixing small volumes for continuous high-throughput flow cytometry: Performance of a mixing Y and peristaltic sample delivery. Cytometry. 47:2002;183-191
13. ® system to on-line mixing assays and demonstrate the first use of a 100-micron stir bar in the sampling line for efficient mixing of particles and reagents in microvolume laminar flow.
14. Young S.M., Curry M.S., Ransom J.T., Ballesteros J.A., Prossnitz E.R., Sklar L.A., Edwards B.S. High-throughput microfluidic mixing and multiparametric cell sorting for bioactive compound screening. J Biomol Screen. 9:2004;103-111 This is the first demonstration of on-line mixing of soluble compounds from microplate wells with continuously flowing cells in conjunction with cell sorting to isolate and characterize physiologically responsive cells.
15. Graves S.W., Nolan J.P., Jett J.H., Martin J.C., Sklar L.A. Nozzle design parameters and their effects on rapid sample delivery in flow cytometry. Cytometry. 47:2002;127-137
16. Truesdell R.A., Vorobieff P.V., Sklar L.A., Mammoli A.A. Mixing of a continuous flow of two fluids due to unsteady flow. Physical Review E. 67.066304:2003;1-11 In this work, the authors characterize a novel approach for mixing in low Reynolds number laminar flow that uses pinch valves in combination with peristaltic action.
17. Fredriksson R., Lagerstrom M.C., Lundin L.G., Schioth H.B. The G-protein-coupled receptors in the human genome form five main families. Phylogenetic analysis, paralogon groups, and fingerprints. Mol Pharmacol. 63:2003;1256-1272
18. Bockaert J., Pin J.P. Molecular tinkering of G protein-coupled receptors: an evolutionary success. EMBO J. 18:1999;1723-1729
19. Wise A., Gearing K., Rees S. Target validation of G-protein coupled receptors. Drug Discov Today. 7:2002;235-246
20. Katugampola S., Davenport A. Emerging roles for orphan G-protein-coupled receptors in the cardiovascular system. Trends Pharmacol Sci. 24:2003;30-35
21. Simons P.C., Shi M., Foutz T., Cimino D.F., Lewis J., Buranda T., Lim W.K., Neubig R.R., McIntire W.E., Garrison J., et al. Ligand-receptor-G-protein molecular assemblies on beads for mechanistic studies and screening by flow cytometry. Mol Pharmacol. 64:2003;1227-1238 The authors describe a bead-based flow cytometry assay system for measuring molecular assemblies between solubilized formyl peptide receptor and G proteins. The system allowed measurement of G protein interaction kinetics and affinities.
22. Simons P.C., Biggs S.M., Waller A., Foutz T., Cimino D.F., Guo Q., Neubig R.R., Tang W.J., Prossnitz E., Sklar L.A. Real-time analysis of ternary complex on particles: direct evidence for partial agonism at the agonist-receptor-G- protein complex assembly step of signal transduction. J Biol Chem. 279:2004;13514-13521 In this work, bead-based flow cytometry assays were extended to the measurement of solubilized β2 adrenergic receptor interacting with ligands and G proteins. The receptor assemblies quantitatively discriminated full and partial agonists and antagonists. A novel duplex assay simultaneously determined whether a test compound was an agonist, antagonist or inactive.
23. Waller A., Simons P., Prossnitz E.R., Edwards B.S., Sklar L.A. High throughput screening of G-protein coupled receptors via flow cytometry. Comb Chem High Throughput Screen. 6:2003;389-397
24. st International Cytomics Conference, International Society for Analytical Cytology, 2003; Newport, Wales.
25. Bartsch JW, Tran HD, Waller A, Mammoli A, Buranda T, Sklar, LA, Edwards BS: An investigation of liquid carryover and sample residual for a high-throughput flow cytometer sample delivery system. Analytical Chemistry 2004, in press.