Targeted patch-clamp recordings and single-cell electroporation of unlabeled neurons in vivo

Nature Methods

Volumn 5, Issue 1, 2008, Pages 61-67

  Kitamura, Kazuo ^a,c,e   Judkewitz, Benjamin ^a   Kano, Masanobu ^b,e   Denk, Winfried ^d   Häusser, Michael ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

^b OSAKA UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^e UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; PLASMID DNA;

ARTICLE; CELL LABELING; CELL STRUCTURE; CELL TYPE; ELECTRODE; ELECTROPORATION; EXTRACELLULAR SPACE; GENETIC MANIPULATION; IN VIVO STUDY; INTRACELLULAR RECORDING; MICROSCOPY; NERVE CELL; PATCH CLAMP; PRIORITY JOURNAL; TRANSGENE;

ANIMALS; CELL MEMBRANE; ELECTROPORATION; MICE; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; NEURONS; PATCH-CLAMP TECHNIQUES; STAINING AND LABELING;

MAMMALIA; RATTUS;

EID: 37749005460     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth1150     Document Type: Article

References (35)

1. Koch, C. & Segev, I. The rote of single neurons in information processing. Nat Neurosci. 3 (suppl), 1171-1177 (2000).
2. Häusser, M. & Mel, B. Dendrites: Bug or feature? Curr. Opin. Neurobiol. 13, 372-383 (2003).
3. Jagadeesh, B., Gray, C.M. & Ferster, D. Visually evoked oscillations of membrane potential in cells of cat visual cortex. Science 257 552-554 (1992).
4. Margie, T.W., Brecht, M. & Sakmann, B. In vivo, low-resistance, whote-cell recordings from neurons in the anaesthetized and awake mammalian brain. Pflugers Arch. 444, 491-498 (2002).
5. Brecht, M., Schneider, M., Sakmann, B. & Margie, T.W. Whisker movements evoked by stimulation of single pyramidal cells in rat motor cortex. Nature 427, 704-710 (2004).
6. Chadderton, P., Margrie, T.W. & Häusser, M. Integration of quanta in cerebellar granule cells during sensory processing. Nature 428 856-860 (2004).
7. Svoboda, K., Denk, W., Kleinfetd, D. & Tank, D.W. In vivo dendritic calcium dynamics in neocortical pyramidal neurons. Nature 385, 161-165 (1997).
8. 2+ influx into dendritic tufts of layer 2/3 neocortical pyramidal neurons in vitro and in vivo. J. Neurosci. 23, 8558-8567 (2003).
9. Helmchen, F. & Denk, W. Deep tissue two-photon microscopy. Nat. Methods 2, 932-940 (2005).
10. Margrie, T.W. et al. Targeted whole-cell recordings in the mammalian brain in vivo. Neuron 39, 911-918 (2003).
11. Dittgen, T. et al. Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo. Proc. Natl. Acad. Sci. USA 101, 18206-18211 (2004).
12. Denk, W. & Detwiler, P.B. Optical recording of Light-evoked calcium signals in the functionally intact retina. Proc Natl. Acad Sci. USA 96, 7035-7040 (1999).
13. Euler, T., Detwiler, P.B. & Denk, W. Directionalty selective calcium signals in dendrites of starhurst amacrine cells. Nature 418 845-852 (2002).
14. Nevian, T. & Helmchen, F. Calcium indicator loading of neurons using single-cell electroporation. Pflugers Arch. 454, 675-688 (2007).
15. Haas, K. et al. Single-cell electroporation for gene transfer in vivo. Neuron 29, 583-591 (2001).
16. Rathenberg, J., Nevian, T. & Witzemann, V. High-efficiency transfection of individual neurons using modified electrophysiology techniques. J. Neurosci. Methods 126, 91-98 (2003).
17. Denk, W., Strickler, J.H. & Webb, W.W. Two-photon laser scanning fluorescence microscopy. Science 248, 73-76 (1990).
18. Stuart, G.J., Dodt, H.U. & Sakmann, B.,Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflugers Arch. 423, 511-518 (1993).
19. 2+ fluorescence imaging with pico-and femtosecond two-photon excitation: Signal and photodamage. Biophys. J. 77, 2226-2236 (1999).
20. Loewenstein, Y. et al. Bistability of cerebellar Purkinje cells modulated by sensory stimulation. Nat. Neurosci. 8, 202-211 (2005).
21. Waters, J. & Helmchen, F. Background synaptic activity is sparse in neocortex. J. Neurosci. 26, 8267-8277 (2006).
22. Stuart, G., Schiller, J. & Sakmann, B. Action potential initiation and propagation in rat neocortical pyramidal neurons. J. Physiol. (Lond.) 505, 617-632 (1997).
23. Svoboda, K., Helmchen, F., Denk, W. & Tank, D.W. Spread of dendritic excitation in Layer 2/3 pyramidal neurons in rat barrel cortex in vivo. Nat Neurosci. 2, 65-73 (1999).
24. + action potentials in distal and terminal dendrites of rat neocortical pyramidal neurons in vitro and in vivo. J. Neurosci. 22, 6991-7005 (2002).
25. Zhu, J.I. & Connors, B.W. Intrinsic firing patterns and whisker-evoked synaptic responses of neurons in the rat barrel cortex. J. Neurophysiol. 81, 1171-1183 (1999).
26. 3 regenerative amplifier. Opt. Lett. 28, 1022-1024 (2003).
27. Jung, J.C. et al. In vivo mammalian brain imaging using one- and two-photon fluorescence microendoscopy, J. Neurophysiol. 92, 3121-3133 (2004).
28. Stosiek, C., Garaschuk, O., Holthoff, K. & Konnerth, A. In vivo two-photon calcium imaging of neuronat networks. Proc. Natl. Acad Sci. USA 100, 7319-7324 (2003).
29. Ohki, K. et al. Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex. Nature 433 597-603 (2005).
30. Miyawaki, A. Innovations in the imaging of brain functions using fluorescent proteins. Neuron 48, 189-199 (2005).
31. 2+ indicator proteins in transgenic mice under TET control. PLoS Biol. 2, e163 (2004).
32. Trachtenberg, J.T. et al. Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex. Nature 420, 788-794 (2002).
33. De Paola, V. et al. Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex. Neuron 49 861-875 (2006).
34. Zhang, F. et al. Circuit-breakers: Optical technologies for probing neural signals and systems. Nat. Rev. Neurosci. 8, 577-581 (2007).
35. Joyner, A.L. Gene Targeting., A Practical Approach 2nd edn. (Oxford Univ. Press, Oxford, UK, 2000).