Lentivirus-based genetic manipulations of cortical neurons and their optical and electrophysiological monitoring in vivo

Proceedings of the National Academy of Sciences of the United States of America

Volumn 101, Issue 52, 2004, Pages 18206-18211

  Dittgen, Tanjew ^a   Nimmerjahn, Axel ^a   Komai, Shoji ^a   Licznerski, Pawel ^a   Waters, Jack ^a   Margrie, Troy W ^b   Helmchen, Fritjof ^a   Denk, Winfried ^a   Brecht, Michael ^a,c   Osten, Pavel ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

^c ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Patch clamp recording; Two photon imaging

Indexed keywords

LENTIVIRUS VECTOR; PROTEIN KINASE (CALCIUM,CALMODULIN) II; SMALL INTERFERING RNA; SYNAPSIN I;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BEHAVIOR; BRAIN CELL; CONTROLLED STUDY; DENDRITIC SPINE; ELECTROPHYSIOLOGY; EMBRYO; FLUORESCENCE MICROSCOPY; GENE FUNCTION; GENETIC MANIPULATION; HUMAN; HUMAN CELL; IN VIVO STUDY; LENTIVIRINAE; MOUSE; NERVE FIBER; NONHUMAN; OPTICS; PATCH CLAMP; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; RAT; SIGNAL TRANSDUCTION; VIRAL GENE DELIVERY SYSTEM;

ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; ELECTROPHYSIOLOGY; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HIPPOCAMPUS; IMAGE PROCESSING, COMPUTER-ASSISTED; LENTIVIRUS; MICE; MICE, INBRED C57BL; MICROSCOPY; NEURONS; PHENOTYPE; PHOTONS; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; RNA, SMALL INTERFERING; RNA, SMALL NUCLEAR; SYNAPSINS;

ANIMALIA; LENTIVIRUS;

EID: 19944429120     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.0407976101     Document Type: Article

References (43)

1. Mayford, M., Mansuy, I. M., Muller, R. U. & Kandel, E. R. (1997) Curr. Biol. 7, R580-R589.
2. Sauer, B. (1998) Methods 14, 381-392.
3. Gossen, M. & Bujard, H. (2002) Annu. Rev. Genet. 36, 153-173.
4. Hubel, D. H. & Wiesel, T. N. (1962) J. Physiol. 160, 106-154.
5. Romo, R. & Salinas, E. (2003) Nat. Rev. Neurosci. 4, 203-218.
6. Cohen, M. R. & Newsome, W. T. (2004) Curr. Opin. Neurobiol. 14, 169-177.
7. Brecht, M., Schneider, M., Sakmann, B. & Margrie, T. W. (2004) Nature 427, 704-710.
8. Kootstra, N. A. & Verma, I. M. (2003) Annu. Rev. Pharmacol. Toxicol. 43, 413-439.
9. Lois, C., Refaeli, Y., Qin, X. F. & Van Parijs, L. (2001) Curr. Opin. Immunol. 13, 496-504.
10. Ailles, L. E. & Naldini, L. (2002) Curr. Top. Microbiol. Immunol. 261, 31-52.
11. Trono, D. (2000) Gene Ther. 7, 20-23.
12. Miyoshi, H., Blomer, U., Takahashi, M., Gage, F. H. & Verma, I. M. (1998) J. Virol. 72, 8150-8157.
13. Lois, C., Hong, E. J., Pease, S., Brown, E. J. & Baltimore, D. (2002) Science 295, 868-872.
14. Niwa, H., Yamamura, K. & Miyazaki, J. (1991) Gene 108, 193-199.
15. Caroni, P. (1997) J. Neurosci. Methods 71, 3-9.
16. Mayford, M., Bach, M. E., Huang, Y. Y., Wang, L., Hawkins, R. D. & Kandel, E. R. (1996) Science 274, 1678-1683.
17. Hoesche, C., Sauerwald, A., Veh, R. W., Krippl, B. & Kilimann, M. W. (1993) J. Biol. Chem. 268, 26494-26502.
18. Thiel, G., Lietz, M. & Cramer, M. (1998) J. Biol. Chem. 273, 26891-26899.
19. Naldini, L., Blomer, U., Gallay, P., Ory, D., Mulligan, R., Gage, F. H., Verma, I. M. & Trono, D. (1996) Science 272, 263-267.
20. Zufferey, R., Nagy, D., Mandel, R. J., Naldini, L. & Trono, D. (1997) Nat. Biotechnol. 15, 871-875.
21. Brewer, G. J., Torricelli, J. R., Evege, E. K. & Price, P. J. (1993) J. Neurosci. Res. 35, 567-576.
22. Yu, J. Y., DeRuiter, S. L. & Turner, D. L. (2002) Proc. Natl. Acad. Sci. USA 99, 6047-6052.
23. Tiscornia, G., Singer, O., Ikawa, M. & Verma, I. M. (2003) Proc. Natl. Acad. Sci. USA 100, 1844-1848.
24. Kleinfeld, D. & Denk, W. (1999) in Imaging Neurons: A Laboratory Manual, eds. Yuste, R., Lanni, F. & Konnerth, A. (Cold Spring Harbor Lab. Press, Plainview, NY), pp. 23.1-23.15.
25. Brecht, M., Roth, A. & Sakmann, B. (2003) J. Physiol. 553, 243-265.
26. Wong-Riley, M. (1979) Brain Res. 171, 11-28.
27. Horikawa, K. & Armstrong, W. E. (1988) J. Neurosci. Methods 25, 1-11.
28. Burgin, K. E., Waxham, M. N., Rickling, S., Westgate, S. A., Mobley, W. C. & Kelly, P. T. (1990) J. Neurosci. 10, 1788-1798.
29. Lendvai, B., Stern, E. A., Chen, B. & Svoboda, K. (2000) Nature 404, 876-881.
30. Trachtenberg, J. T., Chen, B. E., Knott, G. W., Feng, G., Sanes, J. R., Welker, E. & Svoboda, K. (2002) Nature 420, 788-794.
31. Grutzendler, J., Kasthuri, N. & Gan, W. B. (2002) Nature 420, 812-816.
32. Margrie, T. W., Meyer, A. H., Caputi, A., Monyer, H., Hasan, M. T., Schaefer, A. T., Denk, W. & Brecht, M. (2003) Neuron 39, 911-918.
33. Margrie, T. W., Brecht, M. & Sakmann, B. (2002) Pflügers Arch. 444, 491-498.
34. Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K. & Tuschl, T. (2001) Nature 411, 494-498.
35. Qin, X. F., An, D. S., Chen, I. S. & Baltimore, D. (2003) Proc. Natl. Acad. Sci. USA 100, 183-188.
36. Xia, H., Mao, Q., Paulson, H. L. & Davidson, B. L. (2002) Nat. Biotechnol. 20, 1006-1010.
37. Hommel, J. D., Sears, R. M., Georgescu, D., Simmons, D. L. & DiLeone, R. J. (2003) Nat. Med. 9, 1539-1544.
38. Van den Haute, C., Eggermont, K., Nuttin, B., Debyser, Z. & Baekelandt, V. (2003) Hum. Gene Ther. 14, 1799-1807.
39. Rubinson, D. A., Dillon, C. P., Kwiatkowski, A. V., Sievers, C., Yang, L., Kopinja, J., Rooney, D. L., Ihrig, M. M., McManus, M. T., Gertler, F. B., et al. (2003) Nat. Genet. 33, 401-406.
40. Burns, J. C., Friedmann, T., Driever, W., Burrascano, M. & Yee, J. K. (1993) Proc. Natl. Acad. Sci. USA 90, 8033-8037.
41. Feng, G., Mellor, R. H., Bernstein, M., Keller-Peck, C., Nguyen, Q. T., Wallace, M., Nerbonne, J. M., Lichtman, J. W. & Sanes, J. R. (2000) Neuron 28, 41-51.
42. Kordower, J. H., Bloch, J., Ma, S. Y., Chu, Y., Palfi, S., Roitberg, B. Z., Emborg, M., Hantraye, P., Deglon, N. & Aebischer, P. (1999) Exp. Neurol. 160, 1-16.
43. Lai, Z. & Brady, R. O. (2002) J. Neurosci. Res. 67, 363-371.