In vivo, low-resistance, whole-cell recordings from neurons in the anaesthetized and awake mammalian brain

Pflugers Archiv European Journal of Physiology

Volumn 444, Issue 4, 2002, Pages 491-498

  Margrie, Troy W ^a   Brecht, Michael ^a   Sakmann, Bert ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

Author keywords

Anaesthetized; Awake; Barrel cortex; Evoked; In vivo; Olfactory bulb; Spontaneous; Whole cell

Indexed keywords

BUFFER; CALCIUM;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN; BRAIN CORTEX; CELL TYPE; CONTROLLED STUDY; DIALYSIS; ELECTROPHYSIOLOGY; IN VIVO STUDY; MOUSE; NERVE CELL; NERVE CONDUCTION; NERVE POTENTIAL; NEUROPHYSIOLOGY; NONHUMAN; PATCH CLAMP; PRIORITY JOURNAL; RAT; RECORDING; WAKEFULNESS;

ACTION POTENTIALS; ANESTHESIA; ANIMALS; BODY COMPOSITION; BRAIN; BRAIN MAPPING; CONSCIOUSNESS; ELECTRIC IMPEDANCE; ELECTROPHYSIOLOGY; MICE; MICROELECTRODES; NEURONS; PATCH-CLAMP TECHNIQUES; RATS; RATS, WISTAR; VIBRISSAE;

EID: 0036449311     PISSN: 00316768     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00424-002-0831-z     Document Type: Article

References (36)

1. Armstrong-James M, Fox K (1987) Spatiotemporal convergence and divergence in the rat S1 "barrel" cortex. J Comp Neurol 263:265-281
2. Blanton MG, Lo Turco JJ, Kriegstein AR (1989) Whole cell recording from neurons in slices of reptilian and mammalian cerebral cortex. J Neurosci Methods 30:203-210
3. Borg-Graham LJ, Monier C, Fregnac Y (1998) Visual input evokes transient and strong shunting inhibition in visual cortical neurons. Nature 393:369-373
4. Brecht M, Sakmann B (2002) Whisker maps of neuronal subclasses in the rat ventral posterior medial (VPM) thalamus identified by whole-cell voltage recording and morphological reconstruction. J Physiol (Lond) (in press)
5. Chen WR, Ziong W, Shepherd GM (2000) Analysis of relations between NMDA receptors and GABA release at olfactory bulb reciprocal synapses. Neuron 25:625-633
6. Coombs JS, Eccles JC, Fatt P (1955) Excitatory synaptic action in motoneurones. J Physiol (Lond) 130:374-395
7. Covey E, Kauer JA, Casseday JH (1996) Whole-cell patch-clamp recording reveals subthreshold sound-evoked postsynaptic currents in the inferior colliculus of awake bats. J Neurosci 16:3009-3018
8. Diamond ME (1995) Somatosensory thalamus of the rat. In: Jones EG, Diamond IT (eds) The barrel cortex of rodents. Plenum Press, New York, pp 189-219
9. Diamond ME, Armstrong-James M, Ebner FF (1993) Experience-dependent plasticity in adult rat barrel cortex. Proc Natl Acad Sci USA 90:2082-2086
10. Edwards FA, Konnerth A, Sakmann B, Takahashi T (1989) A thin slice preparation for patch clamp recordings from neurones of the mammalian central nervous system. Pflugers Arch 414:600-612
11. Fatt P, Katz B (1951) An analysis of the end-plate potential recorded with an intra-cellular electrode. J Physiol (Lond) 115: 320-370
12. Fee MS (2000) Active stabilization of electrodes for intracel-lular recording in awake behaving animals. Neuron 27:461-468
13. Hamill OP, Marty A, Neher A, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 391:85-100
14. Helmchen F, Svoboda K, Denk W, Tank DW (1999) In vivo dendritic calcium dynamics in deep-layer cortical pyramidal neurons. Nat Neurosci 2:989-996
15. Horikawa K, Armstrong WE (1988) A versatile means of in-tracellular labelling: Injection of biocytin and its detection with avidin conjugates. J Neurosci Methods 25:1-11
16. Huang W, Armstrong-James M, Rema V, Diamond ME, Ebner FF (1989) Contribution of supragranular layers to sensory processing and plasticity in adult rat barrel cortex. J Neurophysiol 80:3261-3271
17. Hubel DH, Wiesel TN (1959) Receptive fields of single neurones in the cat's striate cortex. J Physiol (Lond) 148:574-591
18. Isaacson JS, Strowbridge BW (1998) Olfactory reciprocal synapses: Dendritic signalling in the CNS. Neuron 20:749-761
19. Jagadeesh B, Wheat HS, Ferster D (1993) Linearity of summation of synaptic potentials underlying direction selectivity in simple cells of the cat visual cortex. Science 262:1901-1904
20. Jahr CE, Nicoll RA (1982) An intracellular analysis of dendrodendritic inhibition in the turtle in vitro olfactory bulb. J Physiol (Lond) 326:213-234
21. Jiang M, Griff ER, Ennis M, Zimmer LA, Shipley MT (1996) Activation of locus coeruleus enhances the responses of olfactory bulb mitral cells to weak olfactory nerve input. J Neurosci 16:6319-6329
22. Margrie TW, Sakmann B, Urban NN (2001) Action potential propagation in mitral cell lateral dendrites is decremental and controls recurrent and lateral inhibition in the mammalian olfactory bulb. Proc Natl Acad Sci USA 98:319-324
23. Moore CI, Nelson SB (1998) Spatio-temporal subthreshold receptive fields in the vibrissa representation of rat primary somatosensory cortex. J Neurophysiol 80:2882-2892
24. Neher E (1992) Correction for liquid junction potentials in patch clamp experiments. Methods Enzymol 207:123-131
25. Nelson S, Toth L, Sheth B, Sur M (1994) Orientation selectivity of cortical neurons during intracellular blockade of inhibition. Science 265:774-777
26. Ogden D, Stanfield P (1987) Patch clamp techniques for single channel and whole-cell recording. In: Microelectrode Techniques: The Plymouth Workshop Handbook, 2nd Edn. The Company of Biologists, Cambridge
27. Pei X, Volgushev M, Vidyasagar TR, Creutzfeldt OD (1991) Whole cell recording and conductance measurements in cat visual cortex in-vivo. Neuroreport 2:485-488
28. Rozov A, Burnashev N (1999) Polyamine-dependent facilitation of postsynaptic AMPA receptors counteracts paired-pulse depression. Nature 401:594-598
29. Sah P, Hestrin S, Nicoll RA (1990) Properties of excitatory postsynaptic currents in interneurones in rat hippocampus in vitro. J Physiol (Lond) 430:605-616
30. Sakmann B, Neher E (1983) Geometric parameters of pipettes and membrane patches. In: Sakmann B, Neher E (eds) Single channel recording. Plenum Press, New York
31. Simons DJ (1983) Multi-whisker stimulation and its effects on vibrissa units in rat SmI barrel cortex. Brain Res 276:178-182
32. Stuart GJ, Dodt H-U, Sakmann B (1993) Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy. Pflugers Arch 423:511-518
33. Velumian AA, Zhang L, Carlen PL (1993) A simple method for internal perfusion of mammalian central nervous system neurones in brain slices with multiple solution changes. J Neurosci Methods 48:131-139
34. Walsh RR (1956) Single spike activity in the olfactory bulb. Am J Physiol 186:255-257
35. Young TA, Wilson DA (1999) Frequency-dependent modulation of inhibition in the rat olfactory bulb. Neurosci Lett 276:65-67
36. Zhu JJ, Connors BW (1994) Intrinsic firing patterns and whisker-evoked synaptic responses of neurons in the rat barrel cortex. J Neurophysiol 81:1171-1183