An efficient RNA-cleaving DNA enzyme can specifically target the 5′-untranslated region of severe acute respiratory syndrome associated coronavirus (SARS-CoV)

Journal of Gene Medicine

Volumn 9, Issue 12, 2007, Pages 1080-1086

  Wu, Shuwen ^a   Xu, Junqiang ^a   Liu, Jiangxia ^a   Yan, Xuan ^a   Zhu, Xiaodong ^b   Xiao, Gengfu ^a   Sun, Lunquan ^c   Tien, Po ^a,b  

^a WUHAN UNIVERSITY   (China)

^b INSTITUTE OF MICROBIOLOGY   (China)

^c UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

5 UTR; DNAzyme; Inhibition; SARS CoV

Indexed keywords

COMPLEMENTARY DNA; DEOXYRIBOZYME; ENHANCED GREEN FLUORESCENT PROTEIN; VIRUS RNA;

5' UNTRANSLATED REGION; ANIMAL CELL; ARTICLE; CATALYSIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG MECHANISM; ENZYME SUBSTRATE; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC TRANSFECTION; IN VITRO STUDY; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA CLEAVAGE; RNA EXTRACTION; RNA ISOLATION; RNA SEQUENCE; SARS CORONAVIRUS; VIRUS GENOME; VIRUS INHIBITION; VIRUS REPLICATION; VIRUS STRAIN;

5' UNTRANSLATED REGIONS; ANIMALS; BASE SEQUENCE; CERCOPITHECUS AETHIOPS; DNA; DNA PRIMERS; DNA, CATALYTIC; POLYMERASE CHAIN REACTION; RNA; SARS VIRUS; VERO CELLS;

CORONAVIRUS; MAMMALIA; SARS CORONAVIRUS;

EID: 37349018065     PISSN: 1099498X     EISSN: 15212254     Source Type: Journal    
DOI: 10.1002/jgm.1111     Document Type: Article

References (22)

1. Available: http://www.who.int/csr/sars/en/.
2. Peiris J, Lai S, Poon L, et al. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet 2003; 361: 1319-1325.
3. Marra MA, Jones SJ, Astell CR, et al. The genome sequence of the SARS-associated coronavirus. Science 2003; 300: 1399-1404.
4. Ruan YJ, Wei CL, Ee AL, et al. Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection. Lancet 2003; 361: 1779-1785.
5. Thiel V, Ivanov KA, Putics A, et al. Mechanisms and enzymes involved in SARS coronavirus genome expression. J Gen Virol 2003; 84: 2305-2315.
6. Milligan JF, Matteucci MD, Martin JC. Current concepts in antisense drug design. J Med Chem 1993; 36: 1923-1937.
7. Akhtar S, Agrawal S. In vivo studies with antisense oligonucleotides. Trends Pharmaceut Sci 1997; 18: 12-18.
8. Santoro SW, Joyce GF. A general purpose RNA-cleaving DNA enzyme. Proc Natl Acad Sci USA 1997; 94: 4262-4266.
9. Chakraborti S, Banerjea AC. Inhibition of HIV-1 gene expression by novel DNA enzymes targeted to cleave HIV-1 TAR RNA: potential effectiveness against all HTV-1 isolates. Mol Ther 2003; 6: 817-826.
10. Goila R, Banerjea AC. Sequence specific cleavage of the HIV-1 coreceptor CCR5 gene by a hammer-head ribozyme and a DNA-enzyme: inhibition of the coreceptor function by DNA-enzyme. FEBS Lett 1998; 436: 233-238.
11. Unwalla H, Banerjea AC. Inhibition of HIV-1 gene expression by novel macrophage-tropic DNA enzymes targeted to cleave HIV-1 TAT/Rev RNA. Biochem J 2001; 357: 147-155.
12. Unwalla H, Banerjea AC. Novel mono- and di-DNA-enzymes targeted to cleave TAT or TAT-Rev RNA inhibit HIV-1 gene expression. Antiviral Res 2001; 51: 127-139.
13. Cairns MJ, Hopkins TM, Withering TC, Wang L, Sun LQ. Target site selection for an RNA-cleaving catalytic DNA. Nat Biotechnol 1999; 17: 480-486.
14. Sun LQ, Cairns MJ, Gerlach WL, Witherington C, Wang L, King A. Suppression of smooth muscle cell proliferation by a c-myc RNA-cleaving deoxyribozyme. J Biol Chem 1999; 274: 17236-17241.
15. Cairns MJ, King AK, Sun LQ. Optimization of the 10-23 DNAzyme-substrate paring interactions enhanced RNA cleavage activity at purine-cytosine target sites. Nucleic Acid Res 2003; 31: 2883-2889.
16. Dash BC, Harikrishnan TA, Goila R, et al. Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion. FEBS Lett 1998; 431: 395-399.
17. Santiago FS, Kavurma MM, Lowe HC, et al. New DNA enzyme targeting Egr-1 mRNA inhibits vascular smooth muscle proliferation and regrowth after injury. Nat Med 1999; 5: 1264-1269.
18. Toyoda T, Imamura. Y, Takaku H. Inhibition of influenza virus replication in cultured cells by RNA-cleaving DNA enzyme. FEBS Lett 1999; 458: 151-156.
19. Warashina M, Kuwabara T, Nakamatsu. Y, Taira K. Extremely high and specific activity of DNA enzymes in cells with a Philadelphia chromosome. Chem Biol 1999; 6: 237-250.
20. Yen L, Strittmatter SM, Kalb RG. Sequence-specific cleavage of Huntington mRNA by catalytic DNA. Ann Neurol 1999; 46: 366-373.
21. Zhu Y, Liu M, Zhao W, et al. Isolation of virus from a SARS patient and genome-wide analysis of genetic mutations related to pathogenesis and epidemiology from 47 SARS-CoV isolates. Virus Genes 2005; 30: 93-102.
22. Dass CR, Saravolac EG, Li Y, Sun LQ. Cellular uptake, distribution, and stability of 10-23 deoxyribozymes. Antisense Res Dev 2002; 12: 289-299.