Novel mono- and di-DNA-enzymes targeted to cleave TAT or TAT-REV RNA inhibit HIV-1 gene expression

Antiviral Research

Volumn 51, Issue 2, 2001, Pages 127-139

  Unwalla, Hoshang ^a   Banerjea, Akhil C ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

HIV 1 TAT; REV and ENV; RNA cleaving DNA enzyme

Indexed keywords

DNA; ENZYME; LUCIFERASE; MAGNESIUM CHLORIDE; REV PROTEIN; TRANSACTIVATOR PROTEIN; VIRUS RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DNA CLEAVAGE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SYNTHESIS; GENE EXPRESSION REGULATION; GENE STRUCTURE; GENE THERAPY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MAMMAL CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN TARGETING; STRUCTURE ANALYSIS; VIRUS GENE;

ANIMALS; BASE SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; CELL LINE; CERCOPITHECUS AETHIOPS; DNA, CATALYTIC; DNA, SINGLE-STRANDED; GENE EXPRESSION; GENE PRODUCTS, REV; GENE PRODUCTS, TAT; HELA CELLS; HIV CORE PROTEIN P24; HIV-1; HUMANS; MAGNESIUM CHLORIDE; MOLECULAR SEQUENCE DATA; MUTAGENESIS; RNA, VIRAL;

HUMAN IMMUNODEFICIENCY VIRUS 1; MAMMALIA;

EID: 0034954516     PISSN: 01663542     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-3542(01)00144-9     Document Type: Article

References (23)

1. Production of acquired immunodeficiency syndrome associated retrovirus in human and nonhuman cells transfected with an infectious molecular clone
2. Efficient hammer-head ribozyme-mediated cleavage of the structured hepatitis B virus encapsidation signal in vitro and in cell extracts, but not in intact cells
3. A novel human immunodeficiency virus type 1 protein, tev, shares sequences with tat, env, and rev proteins
4. The chemistry of self-splicing RNA and RNA enzymes
5. Multitarget-ribozyme directed to cleave at up to nine highly conserved HIV-1 env RNA regions inhibits HIV-1 replication- potential effectiveness against most presently sequenced HIV-1 isolates
6. Targeted cleavage of HIV-1 envelope gene by a DNA enzyme and inhibition of HIV-1 envelope-CD4 mediated cell fusion
7. Functional characterization of a U5 ribozyme: Intracellular suppression of human immunodeficiency virus type 1 expression
8. Nuclease-resistant ribozymes decrease stromelysin mRNA levels in rabbit synovium following exogenous delivery to the knee joint
9. Sequence specific cleavage of HIV-1 coreceptor CCR5 gene by a hammer-head ribozyme and a DNA-enzyme: Inhibition of coreceptor function by DNA-enzyme
10. Simple RNA enzymes with new and highly specific endoribonuclease activities
11. A comparison of the in vitro activity of DNA-armed and all-RNA hammer-head ribozymes
12. Control of RNA initiation and elongation at the HIV-1 promoter
13. Characterization of several kinds of dimer mnizyme: Simultaneous cleavage at two sites in HIV-1 tat mRNA by dimer minizymes
14. Inhibition of replication of HIV-1 by retroviral vectors expressing tat-antisense and anti-tat ribozyme RNA
15. Inhibition of human immunodeficiency virus type 1 expression by a hairpin ribozyme
16. Construction and use of a human immunodeficiency virus vector for analysis of infectivity
17. Defective HIV-1 provirus encoding a multitarget-ribozyme inhibits accumulation of spliced and unspliced HIV-1 mRNAs, reduces infectivity of viral progeny, and protects the cells from pathogenesis
18. A general purpose RNA-cleaving DNA enzyme
19. Ribozymes as potential anti-HIV-1 therapeutic agents
20. A nuclease-resistant protein kinase Cα ribozyme blocks glioma cell growth
21. Interactions between equine cyclin T1,Tat, and TAR are disrupted by a leucine-to-valine substitution found in human cyclin T1
22. A small catalytic oligoribonucleotide
23. Expression of ribozymes in gene transfer systems to modulate target RNA levels