Electron microscopic analysis of a fusion protein of postsynaptic density-95 and metallothionein in cultured hippocampal neurons

Journal of Electron Microscopy

Volumn 56, Issue 4, 2007, Pages 119-129

  Fukunaga, Yuko ^a,b   Hirase, Ai ^a,b   Kim, Hyeji ^a   Wada, Natsuko ^a   Nishino, Yuri ^a,b   Miyazawa, Atsuo ^a,b  

^a RIKEN   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Genetically encoded tag; Metallothionein; PSD 95; Transmission electron microscopy

Indexed keywords

CADMIUM; DLGH4 PROTEIN, MOUSE; HYBRID PROTEIN; MEMBRANE PROTEIN; METALLOTHIONEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CELL CULTURE; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; CYTOLOGY; DRUG EFFECT; GENETIC TRANSFECTION; GENETICS; HIPPOCAMPUS; METABOLISM; MOUSE; NERVE CELL; TANDEM REPEAT; TRANSMISSION ELECTRON MICROSCOPY; ULTRASTRUCTURE;

ANIMALS; CADMIUM; CELLS, CULTURED; CERCOPITHECUS AETHIOPS; COS CELLS; HIPPOCAMPUS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; MEMBRANE PROTEINS; METALLOTHIONEIN; MICE; MICROSCOPY, ELECTRON, TRANSMISSION; NEURONS; RECOMBINANT FUSION PROTEINS; TANDEM REPEAT SEQUENCES; TRANSFECTION;

ANTIBODIES; HEAVY METALS;

FUSION PROTEINS; GENETICALLY ENCODED TAG; HIGH RESOLUTION; HIPPOCAMPAL NEURONS; METALLOTHIONEINS; MICROSCOPIC ANALYSIS; POSTSYNAPTIC DENSITIES; POSTSYNAPTIC DENSITY-95; SUBCELLULAR LOCALIZATIONS;

HIGH RESOLUTION TRANSMISSION ELECTRON MICROSCOPY;

EID: 36749027629     PISSN: 00220744     EISSN: 14779986     Source Type: Journal    
DOI: 10.1093/jmicro/dfm027     Document Type: Article

References (22)

1. Chan W C, and Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281: 2016-2018.
2. Michalet X, Pinaud F F, Bentolila L A, Tsay J M, Doose S, Li J J, Sundaresan G, Wu A M, Gambhir S S, and Weiss S (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science 307: 538-544.
3. Giepmans B N, Deerinck T J, Smarr B L, Jones Y Z, and Ellisman MH (2005) Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots. Nat Methods 2: 743-749.
4. Nishino Y, Yasunaga T, and Miyazawa A (2007) A genetically encoded metallothionein tag enabling efficient protein detection by electron microscopy. J. Electron Microsc 56(3): 93-101.
5. Sheng M, and Pak D T (2000) Ligand-gated ion channel interactions with cytoskeletal and signaling proteins. Annu. Rev. Physiol. 62: 755-778.
6. Migaud M, Charlesworth P, Dempster M, Webster L C, Watabe A M, Makhinson M, He Y, Ramsay M F, Morris R G, Morrison J H, et al. (1998) Enhanced long-term potentiation and impaired learning in mice with mutant postsynaptic density-95 protein. Nature 396: 433-439.
7. Tomita S, Nicoll R A, and Bredt D S (2001) PDZ protein interactions regulating glutamate receptor function and plasticity. J. Cell Biol. 153: F19-24.
8. Nielson K B, Atkin C L, and Winge D R (1985) Distinct metal-binding configurations in metallothionein. J. Biol. Chem. 260: 5342-5350.
9. Lopez E, Figueroa S, Oset-Gasque M J, and Gonzalez M P (2003) Apoptosis and necrosis: two distinct events induced by cadmium in cortical neurons in culture. Br. J. Pharmacol. 138: 901-911.
10. Ren X Y, Zhou Y, Zhang J P, Feng W H, and Jiao B H (2003) Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium. World J. Gastroenterol. 9: 1554-1558.
11. Vasconcelos M H, Tam S C, Hesketh J E, Reid M, and Beattie J H (2002) Metal- and tissue-dependent relationship between metallothionein mRNA and protein. Toxicol. Appl. Pharmacol. 182: 91-97.
12. Schmitz G, Minkel D T, Gingrich D, and Shaw C F III (1980) The binding of Gold(I) to metallothionein. J. Inorg. Biochem. 12: 293-306.
13. Mercogliano C P, and DeRosier D J (2006) Gold nanocluster formation using metallothionein: mass spectrometry and electron microscopy. J. Mo.l Biol. 355: 211-223.
14. Mao W P, Ye J L, Guan Z B, Zhao J M, Zhang C, Zhang N N, Jiang P, and Tian T (2007) Cadmium induces apoptosis in human embryonic kidney (HEK) 293 cells by caspase-dependent and -independent pathways acting on mitochondria. Toxicol. In Vitro 21: 343-354.
15. Kagi J H, and Schaffer A (1988) Biochemistry of metallothionein. Biochemistry 27: 8509-8515.
16. Yu X, Wojciechowski M, and Fenselau C (1993) Assessment of metals in reconstituted metallothioneins by electrospray mass spectrometry. Anal. Chem. 65: 1355-1359.
17. Kim E, and Sheng M (2004) PDZ domain proteins of synapses. Nat. Rev. Neurosci. 5: 771-781.
18. Boeckers T (2006) The postsynaptic density. Cell Tissue Res. 326: 409-422.
19. Craven S E, El-Husseini A E, and Bredt D S (1999) Synaptic targeting of the postsynaptic density protein PSD-95 mediated by lipid and protein motifs. Neuron 22: 497-509.
20. Gray N W, Weimer R M, Bureau I, and Svoboda K (2006) Rapid Redistribution of Synaptic PSD-95 in the Neocortex In vivo. PLoS Biol. 4 e370.
21. Aoki C, Miko I, Oviedo H, Mikeladze-Dvali T, Alexandre L, Sweeney N, and Bredt D S (2001) Electron microscopic immunocytochemical detection of PSD-95, PSD-93, SAP-102, and SAP-97 at postsynaptic, presynaptic, and nonsynaptic sites of adult and neonatal rat visual cortex. Synapse 40: 239-257.
22. Valtschanoff J G, and Weinberg R J (2001) Laminar organization of the NMDA receptor complex within the postsynaptic density. J. Neurosci. 21: 1211-1217.