A novel multiple affinity purification tag and its use in identification of proteins associated with a cyclin-CDK complex

Nucleic Acids Research

Volumn 29, Issue 4, 2021, Pages

  Honey, Sangeet ^a   Schneider, Brandt L ^a   Schieltz, David M ^b   Yates, John R ^b   Futcher, Bruce ^a  

^a Howard Hughes Medical Institute Cold Spring Harbor Laboratory Cold Spring Harbor   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN DEPENDENT KINASE; CYCLIN DEPENDENT KINASE 1; CYCLINE; MEMBRANE PROTEIN; PROTEIN CKS1; PROTEIN SIC1; UNCLASSIFIED DRUG; ADENOSINE TRIPHOSPHATASE; CALMODULIN; CALMODULIN BINDING PROTEIN; CELL CYCLE PROTEIN; CKS1 PROTEIN, S CEREVISIAE; CLB2 PROTEIN, S CEREVISIAE; CYCLIN B; FUNGAL PROTEIN; HEAT SHOCK PROTEIN 70; HEMAGGLUTININ; HISTIDINE; HYBRID PROTEIN; MONOCLONAL ANTIBODY; NICKEL; SACCHAROMYCES CEREVISIAE PROTEIN; SIC1 PROTEIN, S CEREVISIAE; SSA1 PROTEIN, S CEREVISIAE;

AFFINITY CHROMATOGRAPHY; ANTIBODY LABELING; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; HOMOZYGOSITY; IMMUNOPRECIPITATION; MASS SPECTROMETRY; MOLECULAR CLONING; MULTIPLE AFFINITY PURIFICATION TAG; NONHUMAN; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; STOP CODON; TANDEM AFFINITY PURIFICATION; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; CHEMISTRY; GENE EXPRESSION REGULATION; GENETICS; IMMUNOLOGY; ISOLATION AND PURIFICATION; MACROMOLECULE; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; SERODIAGNOSIS; WESTERN BLOTTING;

ADENOSINE TRIPHOSPHATASES; AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; BLOTTING, WESTERN; CALMODULIN; CALMODULIN-BINDING PROTEINS; CDC28 PROTEIN KINASE, S CEREVISIAE; CELL CYCLE PROTEINS; CHROMATOGRAPHY, AFFINITY; CYCLIN B; FUNGAL PROTEINS; HEMAGGLUTININS; HISTIDINE; HSP70 HEAT-SHOCK PROTEINS; MACROMOLECULAR SUBSTANCES; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTAGENESIS, INSERTIONAL; NICKEL; PRECIPITIN TESTS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

MLCS; MLOWN;

EID: 0035866279     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/29.4.e24     Document Type: Article

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