Applying a targeted label-free approach using LC-MS AMT tags to evaluate changes in protein phosphorylation following phosphatase inhibition

Journal of Proteome Research

Volumn 6, Issue 11, 2007, Pages 4489-4497

  Yang, Feng ^a   Jaitly, Navdeep ^a   Jayachandran, Hemalatha ^b   Luo, Quanzhou ^a   Monroe, Matthew E ^a   Du, Xiuxia ^a   Gritsenko, Marina A ^a   Zhang, Rui ^a   Anderson, David J ^a   Purvine, Samuel O ^a   Adkins, Joshua N ^a   Moore, Ronald J ^a   Mottaz, Heather M ^a   Ding, Shi Jian ^a   Lipton, Mary S ^a   Camp II, David G ^a   Udseth, Harold R ^a   Smith, Richard D ^a   Rossie, Sandra ^b,c  

^a PACIFIC NORTHWEST NATIONAL LABORATORY   (United States)

^b Purdue University   (United States)

^c PURDUE UNIVERSITY   (United States)

Author keywords

20 m ID monolithic column; AMT tag pipeline; Comparative phosphoproteomics; Immobilized metal ion affinity chromatography (IMAC); Label free quantitation; Mass spectrometry; Phosphoprotein phosphatase (PPP) family; Targeted MS MS

Indexed keywords

A KINASE ANCHORING PROTEIN 8; CALYCULIN A; CATENIN; CYCLIC AMP DEPENDENT PROTEIN KINASE ANCHORING PROTEIN; CYTOCHROME P450C17; PHOSPHOPROTEIN PHOSPHATASE; STATHMIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN TARGETING; PROTEOMICS; UPREGULATION;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, ION EXCHANGE; CHROMATOGRAPHY, LIQUID; ENZYME INHIBITORS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HELA CELLS; HUMANS; MASS SPECTROMETRY; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; OXAZOLES; PEPTIDES; PHOSPHOPEPTIDES; PHOSPHORYLATION; PROTEOMICS;

EID: 36348975222     PISSN: 15353893     EISSN: None     Source Type: Journal    
DOI: 10.1021/pr070068e     Document Type: Article

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