DNA fragmentation based combinatorial approaches to soluble protein expression. Part II: Library expression, screening and scale-up

Drug Discovery Today

Volumn 12, Issue 21-22, 2007, Pages 939-947

  Savva, Renos ^a,d   Prodromou, Chrisostomos ^b,d   Driscoll, Paul C ^c,d  

^a UNIVERSITY OF LONDON   (United Kingdom)

^b INSTITUTE OF CANCER RESEARCH   (United Kingdom)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

^d Domainex Ltd   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PEPTIDE FRAGMENT; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN; PROTEIN P85; PROTEIN SIR3; RNA POLYMERASE; TELOMERASE REVERSE TRANSCRIPTASE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; BIOTINYLATION; CARBOXY TERMINAL SEQUENCE; CENTRIFUGATION; CIRCULAR DICHROISM; COMBINATORIAL LIBRARY; DNA LIBRARY; DNA TEMPLATE; DRUG RESEARCH; ELECTROSPRAY MASS SPECTROMETRY; ENZYME SUBUNIT; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; GENE DELETION; GENE EXPRESSION; GENETIC CODE; HIGH THROUGHPUT SCREENING; HYDROPHILICITY; IMMUNOBLOTTING; INFLUENZA VIRUS; LIGHT SCATTERING; MOLECULAR DYNAMICS; MUTAGENESIS; NUCLEAR MAGNETIC RESONANCE; POLYMERASE CHAIN REACTION; PROTEIN AGGREGATION; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PROCESSING; PROTEIN SECONDARY STRUCTURE; REVIEW; SCALE UP; SCREENING; SEDIMENTATION; SOLUBILITY; SOLUBILIZATION; ULTRACENTRIFUGATION;

1-PHOSPHATIDYLINOSITOL 3-KINASE; COMBINATORIAL CHEMISTRY TECHNIQUES; DNA FRAGMENTATION; GENE LIBRARY; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEINS; SILENT INFORMATION REGULATOR PROTEINS, SACCHAROMYCES CEREVISIAE; SOLUBILITY; TELOMERASE; VIRAL PROTEINS;

EID: 35748961085     PISSN: 13596446     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.drudis.2007.08.016     Document Type: Review

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