Engineering and rapid selection of a low-affinity glucose/galactose-binding protein for a glucose biosensor

Protein Science

Volumn 16, Issue 11, 2007, Pages 2350-2359

  Amiss, Terry J ^a   Sherman, Douglas B ^a   Nycz, Colleen M ^a   Andaluz, Sandra A ^a   Pitner, J Bruce ^a  

^a BD Technologies   (United States)

Author keywords

Focused library; GGBP; Glucose galactose binding protein; Low affinity; Screening; Structure based site saturation mutagenesis

Indexed keywords

ARGININE; GALAPTIN; GLUCOSE;

ARTICLE; BINDING AFFINITY; BIOSENSOR; CONFORMATIONAL TRANSITION; DIABETES MELLITUS; DISSOCIATION CONSTANT; ENTROPY; GLUCOSE TRANSPORT; HYDROGEN BOND; PRIORITY JOURNAL; PROTEIN BINDING; SCREENING;

BINDING SITES; BIOSENSING TECHNIQUES; CALCIUM-BINDING PROTEINS; CLONING, MOLECULAR; ESCHERICHIA COLI; FLUORESCENT DYES; GENE LIBRARY; GLUCOSE; HUMANS; HYDROGEN BONDING; MOLECULAR CONFORMATION; MONOSACCHARIDE TRANSPORT PROTEINS; MUTAGENESIS; MUTATION; PERIPLASMIC BINDING PROTEINS; PROTEIN BINDING; PROTEIN ENGINEERING;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 35648996014     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.073119507     Document Type: Article

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