Expression of hepatitis B surface antigen major subtypes in Pichia pastoris and purification for in vitro diagnosis

Protein Expression and Purification

Volumn 56, Issue 2, 2007, Pages 177-188

  Ottone, Sophie ^a   Nguyen, Xuan ^a   Bazin, Julien ^a   Bérard, Cédric ^a   Jimenez, Sandrine ^a   Letourneur, Odile ^a  

^a BIOMÉRIEUX   (France)

Author keywords

Anti HBs antibody; Diagnosis; Hepatitis B surface antigen; Hepatitis B virus; Immunoassay; Pichia pastoris

Indexed keywords

HEPATITIS B SURFACE ANTIGEN; MONOCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ARTICLE; BIOREACTOR; BIOSYNTHESIS; CELL CULTURE; FERMENTATION; GENE VECTOR; GENETICS; HEPATITIS B; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PICHIA; PROTEIN ENGINEERING;

ANTIBODIES, MONOCLONAL; BIOREACTORS; CELLS, CULTURED; FERMENTATION; GENETIC VECTORS; HEPATITIS B; HEPATITIS B SURFACE ANTIGENS; PICHIA; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

HEPATITIS B VIRUS; PICHIA PASTORIS;

EID: 35548972030     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.07.008     Document Type: Article

References (38)

1. Lavanchy D. Worldwide epidemiology of HBV infection, disease burden, and vaccine prevention. J. Clin. Virol. 34 Suppl. 1 (2005) S1-S3
2. Huang C.F., Lin S.S., Ho Y.C., Chen F.L., and Yang C.C. The immune response induced by hepatitis B virus principal antigens. Cell. Mol. Immunol. 3 (2006) 97-106
3. Dane D.S., Cameron C.H., and Briggs M. Virus-like particles in serum of patients with Australia-antigen-associated hepatitis. Lancet 1 (1970) 695-698
4. Chisari F.V., and Ferrari C. Hepatitis B virus immunopathogenesis. Annu. Rev. Immunol. 13 (1995) 29-60
5. Magnius L.O., and Norder H. Subtypes, genotypes and molecular epidemiology of the hepatitis B virus as reflected by sequence variability of the S-gene. Intervirology 38 (1995) 24-34
6. Norder H., Courouce A.M., Coursaget P., Echevarria J.M., Lee S.D., Mushahwar I.K., Robertson B.H., Locarnini S., and Magnius L.O. Genetic diversity of hepatitis B virus strains derived worldwide: genotypes, subgenotypes, and HBsAg subtypes. Intervirology 47 (2004) 289-309
7. Norder H., Hammas B., Lofdahl S., Courouce A.M., and Magnius L.O. Comparison of the amino acid sequences of nine different serotypes of hepatitis B surface antigen and genomic classification of the corresponding hepatitis B virus strains. J. Gen. Virol. 73 Pt 5 (1992) 1201-1208
8. Yu A.S., Cheung R.C., and Keeffe E.B. Hepatitis B vaccines. Clin Liver Dis 8 (2004) 283-300
9. Michel M.L., Pontisso P., Sobczak E., Malpiece Y., Streeck R.E., and Tiollais P. Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin. Proc. Natl. Acad. Sci. USA 81 (1984) 7708-7712
10. Valenzuela P., Medina A., Rutter W.J., Ammerer G., and Hall B.D. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast. Nature 298 (1982) 347-350
11. Heijtink R.A., Bergen P., Melber K., Janowicz Z.A., and Osterhaus A.D. Hepatitis B surface antigen (HBsAg) derived from yeast cells (Hansenula polymorpha) used to establish an influence of antigenic subtype (adw2, adr, ayw3) in measuring the immune response after vaccination. Vaccine 20 (2002) 2191-2196
12. Gerlich W.H., Deepen R., Heermann K.H., Krone B., Lu X.Y., Seifer M., and Thomssen R. Protective potential of hepatitis B virus antigens other than the S gene protein. Vaccine 8 Suppl. (1990) S63-S68
13. Neurath A.R., Kent S.B., Strick N., Taylor P., and Stevens C.E. Hepatitis B virus contains pre-S gene-encoded domains. Nature 315 (1985) 154-156
14. Hellwig S., Emde F., Raven N.P., Henke M., van der Logt P., and Fischer R. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 74 (2001) 344-352
15. Paulij W.P., de Wit P.L., Sunnen C.M., van Roosmalen M.H., Petersen-van Ettekoven A., Cooreman M.P., and Heijtink R.A. Localization of a unique hepatitis B virus epitope sheds new light on the structure of hepatitis B virus surface antigen. J. Gen. Virol. 80 Pt 8 (1999) 2121-2126
16. Kidd-Ljunggren K., Courouce A.M., Oberg M., and Kidd A.H. Genetic conservation within subtypes in the hepatitis B virus pre-S2 region. J. Gen. Virol. 75 Pt 6 (1994) 1485-1490
17. Sreekrishna K., Brankamp R.G., Kropp K.E., Blankenship D.T., Tsay J.T., Smith P.L., Wierschke J.D., Subramaniam A., and Birkenberger L.A. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene 190 (1997) 55-62
18. Cereghino G.P., Cereghino J.L., Ilgen C., and Cregg J.M. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13 (2002) 329-332
19. McGrew J.T., Leiske D., Dell B., Klinke R., Krasts D., Wee S.F., Abbott N., Armitage R., and Harrington K. Expression of trimeric CD40 ligand in Pichia pastoris: use of a rapid method to detect high-level expressing transformants. Gene 187 (1997) 193-200
20. Tleugabulova D., Falcon V., and Penton E. Physico-chemical characterization of recombinant hepatitis B surface antigen by a multidimensional approach. J. Chromatogr. A 845 (1999) 171-179
21. Hardy E., Martinez E., Diago D., Diaz R., Gonzalez D., and Herrera L. Large-scale production of recombinant hepatitis B surface antigen from Pichia pastoris. J. Biotechnol. 77 (2000) 157-167
22. Wampler D.E., Lehman E.D., Boger J., McAleer W.J., and Scolnick E.M. Multiple chemical forms of hepatitis B surface antigen produced in yeast. Proc. Natl. Acad. Sci. USA 82 (1985) 6830-6834
23. Langley K.E., Egan K.M., Barendt J.M., Parker C.G., and Bitter G.A. Characterization of purified hepatitis B surface antigen containing pre-S(2) epitopes expressed in Saccharomyces cerevisiae. Gene 67 (1988) 229-245
24. Holzer G.W., Mayrhofer J., Leitner J., Blum M., Webersinke G., Heuritsch S., and Falkner F.G. Overexpression of hepatitis B virus surface antigens including the preS1 region in a serum-free Chinese hamster ovary cell line. Protein Expr. Purif. 29 (2003) 58-69
25. Yamada T., Iwabuki H., Kanno T., Tanaka H., Kawai T., Fukuda H., Kondo A., Seno M., Tanizawa K., and Kuroda S. Physicochemical and immunological characterization of hepatitis B virus envelope particles exclusively consisting of the entire L (pre-S1 + pre-S2 + S) protein. Vaccine 19 (2001) 3154-3163
26. de Roubin M.R., Bastien L., Shen S.H., and Groleau D. Fermentation study for the production of hepatitis B virus pre-S2 antigen by the methylotrophic yeast Hansenula polymorpha. J. Ind. Microbiol. 8 (1991) 147-156
27. Cregg J.M., Tschopp J.F., Stillman C., Siegel R., Akong M., Craig W.S., Buckholz K.R., Madden K.R., Kellaris P.A., Davis G.R., Smiley B.L., Cruze J., Torregrossa R., Velicebeti G., and Thill G.P. High-level expression and efficient assembly of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris. Bio/technologie 5 (1987) 479-485
28. Vassileva A., Chugh D.A., Swaminathan S., and Khanna N. Expression of hepatitis B surface antigen in the methylotrophic yeast Pichia pastoris using the GAP promoter. J. Biotechnol. 88 (2001) 21-35
29. Hamsa P.V., and Chattoo B.B. Cloning and growth-regulated expression of the gene encoding the hepatitis B virus middle surface antigen in Yarrowia lipolytica. Gene 143 (1994) 165-170
30. G.P. Thill, Expression of hepatitis B preS2 protein in methylotrophic yeasts, Eur. Pat. Appli. 89107457.7, 1989.
31. Kobayashi M., Asano T., Utsunomiya M., Itoh Y., Fujisawa Y., Nishimura O., Kato K., and Kakinuma A. Recombinant hepatitis B virus surface antigen carrying the pre-S2 region derived from yeast: purification and characterization. J. Biotechnol. 8 (1988) 1-22
32. Agraz A., Duarte C.A., Costa L., Perez L., Paez R., Pujol V., and Fontirrochi G. Immunoaffinity purification of recombinant hepatitis B surface antigen from yeast using a monoclonal antibody. J. Chromatogr. A 672 (1994) 25-33
33. Valdes R., Reyes B., Alvarez T., Garcia J., Montero J.A., Figueroa A., Gomez L., Padilla S., Geada D., Abrahantes M.C., Dorta L., Fernandez D., Mendoza O., Ramirez N., Rodriguez M., Pujol M., Borroto C., and Brito J. Hepatitis B surface antigen immunopurification using a plant-derived specific antibody produced in large scale. Biochem. Biophys. Res. Commun. 310 (2003) 742-747
34. Ibarra N., Caballero A., Gonzalez E., and Valdes R. Comparison of different elution conditions for the immunopurification of recombinant hepatitis B surface antigen. J. Chromatogr. B Biomed. Sci. Appl. 735 (1999) 271-277
35. da Silva e Mouta Jr. S., Otavio A.V.C., Ennes I., de Andrade G.S., da Silva F.M., Domingos D.S.E., Giovanni D.S.S., and Terezinha Baroni D.M.M. Simple immunoaffinity method to purify recombinant hepatitis B surface antigen secreted by transfected mammalian cells. J. Chromatogr. B. Analyt. Technol. Biomed. Life. Sci. 787 (2003) 303-311
36. Imamura T., Araki M., Miyanohara A., Nakao J., Yonemura H., Ohtomo N., and Matsubara K. Expression of hepatitis B virus middle and large surface antigen genes in Saccharomyces cerevisiae. J. Virol. 61 (1987) 3543-3549
37. Itoh Y., and Fujisawa Y. Synthesis in yeast of hepatitis B virus surface antigen modified P31 particles by gene modification. Biochem. Biophys. Res. Commun. 141 (1986) 942-948
38. Schmitt S., Glebe D., Tolle T.K., Lochnit G., Linder D., Geyer R., and Gerlich W.H. Structure of pre-S2 N- and O-linked glycans in surface proteins from different genotypes of hepatitis B virus. J. Gen. Virol. 85 (2004) 2045-2053