Cloning, expression, purification, and characterisation of the dUTPase encoded by the integrated Bacillus subtilis temperate bacteriophage SPβ

Protein Expression and Purification

Volumn 42, Issue 1, 2005, Pages 92-99

  Persson, Rebecca ^a   McGeehan, John ^a,b   Wilson, Keith S ^a  

^a UNIVERSITY OF YORK   (United Kingdom)

^b UNIVERSITY OF PORTSMOUTH   (United Kingdom)

Author keywords

Bacillus subtilis; dUTPase; Protein expression; SP

Indexed keywords

DEOXYURIDINE PHOSPHATE; DIVALENT CATION; DUTP PYROPHOSPHATASE; INORGANIC PYROPHOSPHATASE; RECOMBINANT PROTEIN; VIRUS PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIOPHAGE; BIOSYNTHESIS; CHEMISTRY; CRYSTALLIZATION; ENZYMOLOGY; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOELECTRIC POINT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; VIROLOGY;

AMINO ACID SEQUENCE; BACILLUS SUBTILIS; BACTERIOPHAGES; CATIONS, DIVALENT; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CRYSTALLIZATION; DEOXYURACIL NUCLEOTIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GENETIC VECTORS; ISOELECTRIC POINT; KINETICS; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PYROPHOSPHATASES; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SEQUENCE HOMOLOGY, AMINO ACID; VIRAL PROTEINS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 20444365191     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.02.013     Document Type: Article

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