Optimization of the Gβ1 Domain by Computational Design and by in Vitro Evolution: Structural and Energetic Basis of Stabilization

Journal of Molecular Biology

Volumn 373, Issue 3, 2007, Pages 775-784

  Wunderlich, Michael ^a   Max, Klaas E A ^b   Roske, Yvette ^b   Mueller, Uwe ^c   Heinemann, Udo ^b,d   Schmid, Franz X ^a  

^a UNIVERSITY OF BAYREUTH   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c HAHN MEITNER INSTITUT   (Germany)

^d FREIE UNIVERSITÄT BERLIN   (Germany)

Author keywords

computational protein design; in vitro selection; phage display; protein stability; streptococcal protein G

Indexed keywords

BACTERIAL PROTEIN; PROTEIN G BETA 1; UNCLASSIFIED DRUG; BACTERIAL ANTIGEN; IGG FC BINDING PROTEIN, STREPTOCOCCUS; IGG FC-BINDING PROTEIN, STREPTOCOCCUS;

AMINO TERMINAL SEQUENCE; ARTICLE; BETA SHEET; CARBOXY TERMINAL SEQUENCE; CRYSTAL STRUCTURE; ENERGY TRANSFER; MUTATION; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN STABILITY; PROTEIN STRUCTURE; PROTEIN VARIANT; THERMODYNAMICS; BIOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; COMPUTER SIMULATION; GENETICS; METABOLISM; MOLECULAR EVOLUTION; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; COMPUTATIONAL BIOLOGY; COMPUTER SIMULATION; CRYSTALLOGRAPHY, X-RAY; EVOLUTION, MOLECULAR; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; THERMODYNAMICS;

EID: 34748819310     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2007.08.004     Document Type: Article

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