Construction of gene-targeting vectors: A rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles

Transgenic Research

Volumn 10, Issue 1, 2001, Pages 69-80

  Vilen, H ^a   Eerikainen S ^a   Tornberg, J ^a   Airaksinen, M S ^a   Savilahti, H ^a  

^a Viikki Biocenter   (Finland)

Author keywords

Conditional and hypomorphic alleles; DNA transposition; Gene targeting; Kcc2 gene; Phage Mu; Transgenic mice

Indexed keywords

GENE FUNCTION; GENE SEQUENCE; GENE TARGETING; GENETIC RECOMBINATION; HOMOLOGOUS RECOMBINATION; PHAGE VECTOR; PLASMID VECTOR; PROCESS DESIGN; TRANSGENIC MOUSE;

ALLELES; ANIMALS; BACTERIOPHAGE MU; BLOTTING, WESTERN; CARRIER PROTEINS; CELLS, CULTURED; DNA NUCLEOTIDYLTRANSFERASES; DNA PRIMERS; DNA TRANSPOSABLE ELEMENTS; ELECTROPORATION; GENE EXPRESSION REGULATION; GENE TARGETING; GENETIC VECTORS; MICE; MICE, INBRED C57BL; MICE, TRANSGENIC; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; RESTRICTION MAPPING; SYMPORTERS;

ANIMALIA; ENTEROBACTERIA PHAGE MU; MAMMALIA; MUS MUSCULUS;

EID: 0035120076     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008959231644     Document Type: Article

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