Efficient insertion mutagenesis strategy for bacterial genomes involving electroporation of in vitro-assembled DNA transposition complexes of bacteriophage Mu

Applied and Environmental Microbiology

Volumn 68, Issue 2, 2002, Pages 705-712

  Lamberg, Arja ^a   Nieminen, Sari ^a   Qiao, Mingqiang ^a   Savilahti, Harri ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ESCHERICHIA COLI; GENES; PROTEINS;

INCUBATION;

MUTAGENESIS;

ANTIBIOTIC AGENT; BACTERIAL DNA; CHLORAMPHENICOL; KANAMYCIN; PLASMID DNA;

BACTERIUM;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIOPHAGE; BACTERIUM CULTURE; CONTROLLED STUDY; DNA TRANSPOSITION; ESCHERICHIA COLI; GENE INSERTION; GENOME; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PECTOBACTERIUM CAROTOVORUM; SALMONELLA ENTERICA; TRANSPOSON; UNINDEXED SEQUENCE; YERSINIA ENTEROCOLITICA;

BACTERIOPHAGE MU; BASE SEQUENCE; DNA TRANSPOSABLE ELEMENTS; ELECTROPORATION; GENOME, BACTERIAL; GRAM-NEGATIVE BACTERIA; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL;

BACTERIA (MICROORGANISMS); ENTEROBACTERIA PHAGE MU; ERWINIA; ESCHERICHIA COLI; INSERTION SEQUENCES; NEGIBACTERIA; PECTOBACTERIUM CAROTOVORUM; PROKARYOTA; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR TYPHIMURIUM; TRANSPOSONS; UNIDENTIFIED BACTERIOPHAGE; YERSINIA; YERSINIA ENTEROCOLITICA;

EID: 0036156745     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.68.2.705-712.2002     Document Type: Article

References (44)

1. Transposition mediated by RAG1 and RAG2 and its implications for the evolution of the immune system
2. Systematic identification of essential genes by in vitro mariner mutagenesis
3. Mu insertion duplicates a 5 base pair sequence at host inserted site
4. Transposable elements as tools for molecular analysis in bacteria
5. A simple in vitro Tn 7-based transposition system with low target site selectivity for genome and gene analysis
6. A complementation analysis of the restriction and modification of DNA in Escherichia coli
7. DNA transposition: Jumping gene machine, some assembly required
8. Tn 1O/IS 10 transposase purification, activation, and in vitro reaction
9. In vitro transposition system for efficient generation of random mutants of Campylobacterjejuni
10. Unity in transposition reactions
11. Transposition of Mu DNA: Joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu
12. Efficient integration of artificial transposons into plasmid targets in vitro: A useful tool for DNA mapping, sequencing and genetic analysis
13. Tn 5 in vitro transposition
14. Insertional transposon mutagenesis by electroporation of released Tn 5 transposition complexes
15. Differential plasmid rescue from transgenic mouse DNAs into Escherichia coli methylation-restriction mutants
16. In vitro transposition of Tn 552: A tool for DNA sequencing and mutagenesis
17. In vitro Tn 7 mutagenesis of Haemophilus influenzae Rd and characterization of the role of atpA in transformation
18. An efficient and accurate integration of mini-Mu transposons in vitro: A general methodology for functional genetic analysis and molecular biology applications
19. An efficient DNA sequencing strategy based on the bacteriophage Mu in vitro DNA transposition reaction
20. Pentapeptide scanning mutagenesis: Encouraging old proteins to execute unusual tricks
21. DNA transposition by the RAG1 and RAG2 proteins: A possible source of oncogenic translocations
22. Transposome insertional mutagenesis and direct sequencing of microbial genomes
23. Nucleotide sequences of the attachment sites of bacteriophage Mu
24. Eukaryotic transposable elements as tools to study gene structure and function
25. Binding of an Escherichia coli double-stranded DNA virus PRDI to a receptor coded by an IncP-type plasmid
26. Functional characterization of the human immunodeficiency virus type 1 genome by genetic footprinting
27. Bacteriophage lambda cloning system for the construction of directional cDNA libraries
28. Transpositional recombination: Mechanistic insights from studies of Mu and other elements
29. Mu transpositional recombination: Donor DNA cleavage and strand transfer in trans by the Mu transposase
30. The phage Mu transpososome core: DNA requirements for assembly and function
31. High-resolution functional mapping of a cloned gene by genetic footprinting
32. Lack of correlation between the presence of plasmids and fimbriae in Yersinia enterocolitica and Yersinia pseudotuberculosis
33. Genetic footprinting: A genomic strategy for determining a gene's function given its sequence
34. Use of T7 RNA polymerase to direct expression of cloned genes
35. Functional genomics of Neisseria meningitidis pathogenesis
36. Transpososomes: Stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA
37. Mutational analysis of the Pseudomonas syringae pv. tomato hrpA gene encoding Hrp pilus subunit
38. cis and trans factors affecting Mosl mariner evolution and transposition in vitro, and its potential for functional genomics
39. Construction of gene-targeting vectors: A rapid Mu in vitro DNA transposition-based strategy generating null, potentially hypomorphic, and conditional alleles
40. A large nucleoprotein assembly at the ends of the viral DNA mediates retroviral DNA integration
41. Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa