Global analysis of protein-protein interactions reveals multiple CYP2E1-reductase complexes

Biochemistry

Volumn 46, Issue 35, 2007, Pages 10192-10201

  Jamakhandi, Arvind P ^a   Kuzmic, Petr ^b   Sanders, Daniel E ^a   Miller, Grover P ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b BioKin Ltd   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYST ACTIVITY; COMPLEXATION; CONCENTRATION (PROCESS); DIMERS; TITRATION;

BINARY FUNCTIONAL COMPLEX; BINDING MODELS; HOMODIMERS; TITRATION CURVES;

ENZYME KINETICS;

CYTOCHROME P450 2E1; CYTOCHROME P450 REDUCTASE;

ARTICLE; CATALYSIS; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); ENZYME ACTIVITY; ENZYME MECHANISM; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

AMINO ACID SUBSTITUTION; ANIMALS; COMPUTER SIMULATION; CYTOCHROME P-450 CYP2B1; CYTOCHROME P-450 CYP2E1; GLUTAMINE; LYSINE; MATHEMATICAL COMPUTING; MICROSOMES, LIVER; MODELS, BIOLOGICAL; MODELS, CHEMICAL; MULTIENZYME COMPLEXES; MUTATION; NADPH-FERRIHEMOPROTEIN REDUCTASE; NITROPHENOLS; OXIDATION-REDUCTION; PROTEIN BINDING; RABBITS; RECOMBINANT PROTEINS; THERMODYNAMICS; TITRIMETRY;

EID: 34548528384     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi7003476     Document Type: Article

References (40)

1. Kuzmic, P. (2006) A generalized matrix formalism for steady state enzyme kinetics: Application to 17beta-HSD, Mol. Cell. Endocrinol. 248, 172-181.
2. Porter, T. D., and Coon, M. J. (1991) Cytochrome P-450: multiplicity of isoforms, substrates, and catalytic and regulatory mechanisms, J. Biol. Chem. 266, 13469-13472.
3. Hlavica, P., Schulze, J., and Lewis, D. F. (2003) Functional interaction of cytochrome P450 with its redox partners: a critical assessment and update of the topology of predicted contact regions, Inorg. Biochem. 96, 279-297.
4. Estabrook, R., Franklin, M. R., Cohen, B., Shigamatzu, A., and Hildebrandt, A. G. (1971) Biochemical and genetic factors influencing drug metabolism. Influence of hepatic microsomal mixed function oxidation reactions on cellular metabolic control, Metabolism 20, 187-199.
5. Watanabe, J., Asaka, Y., Fujimoto, S., and Kanamura, S. (1993) Densities of NADPH-ferrihemoprotein reductase and cytochrome P-450 molecules in the endoplasmic reticulum membrane of rat hepatocytes, J. Histochem. Cytochem. 42, 43-49.
6. Gut, J., Richter, C., Cherry, R. J., Winterhalter, K. H., and Kawato, S. (1983) Rotation of cytochrome P-450: complex formation of cytochrome P-450 with NADPH-cytochrome P-450 reductase in liposomes demonstrated by combining protein rotation with antibody-induced cross-linking, J. Biol. Chem. 258, 8588-8594.
7. Schwarz, D., Pirrwitz, J., and Ruckpaul, K. (1982) Rotational diffusion of cytochrome P-450 in the microsomal membrane-evidence for a clusterlike organization from saturation transfer electron paramagnetic resonance spectroscopy, Arch. Biochem. Biophys. 216, 322-328.
8. 5, and NADPH-cytochrome P-450 reductase of hepatic microsomes, Biochem. Biophys. Res. Commun. 134, 519-526.
9. 5, cytochrome c, and cytochrome P-450 interactions with NADPH-cytochrome P-450 reductase in phospholipid vesicles, Biochemistry 27, 5869-5876.
10. Yang, C. S., and Strickhart, F. S. (1975) Interactions between solubilized cytochrome P-450 and hepatic microsomes, J. Biol. Chem. 250, 7968-7972.
11. Yang, C. S., Strickhart, F. S., and Kicha, L. P. (1978) Interaction between NADPH-cytochrome P-450 reductase and hepatic microsomes, Biochim. Biophys. Acta 509, 326-337.
12. Miwa, G. T., West, S. B., Huang, M. T., and Lu, A. Y. (1979) Studies on the association of cytochrome P-450 and NADPH-cytochrome c reductase during catalysis in a reconstituted hydroxylating system, J. Biol. Chem. 254, 5695-5700.
13. Guengerich, F. P., Kim, D. H., and Iwasaki, M. (1991) Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspects, Chem. Res. Toxicol. 4, 168-79.
14. Ronis, M., Lindros, K. O., and Ingelman-Sundberg, M. (1996) The CYP2E1 subfamily, in Cytochromes P450-metabolic and toxicological aspects (Ioannides, C., Ed.) pp 211-239, CRC Press, Boca Raton, FL.
15. 5 in the oxidation of 7-ethoxycoumarin, chlorzoxazone, aniline, and N-nitrosodimethylamine by recombinant cytochrome P450 2E1 and by human liver microsomes, Biochem. Pharmacol. 52, 301-309.
16. Lewis, D. F., Bird, M. G., and Parke, D. V. (1997) Molecular modelling of CYP2E1 enzymes from rat, mouse and man: an explanation for species differences in butadiene metabolism and potential carcinogenicity, and rationalization of CYP2E substrate specificity, Toxicology 118, 93-113.
17. Bonina, T. A., Gilep, A. A., Estabrook, R. W., and Usanov, S. A. (2005) Engineering of proteolytically stable NADPH-cytochrome P450 reductase, Biochemistry (Moscow) 70, 357-365.
18. Collom, S. L., Jamakhandi, A. P., Tackett, A. J., Radominska-Pandya, A., and Miller, G. P. (2007) CYP2E1 active site residues in substrate recognition sequence 5 identified by photoaffinity labeling and homology modeling, Arch. Biochem. Biophys. 459, 59-69.
19. Backes, W. L., Batie, C. J., and Cawley, G. F. (1998) Interactions among P450 enzymes when combined in reconstituted systems: formation of a 2B4-1A2 complex with a high affinity for NADPH-cytochrome P450 reductase, Biochemistry 37, 12852-12859.
20. Hazai, E., Bikadi, Z., Simonyi, M., and Kupfer, D. (2005) Association of cytochrome P450 enzymes is a determining factor in their catalytic activity, J. Comput.-Aided Mol. Des. 19, 271-85.
21. Cheng, D., Kelley, R. W., Cawley, G. F., and Backes, W. L. (2004) High-level expression of recombinant rabbit cytochrome P450 2E1 in Escherichia coli C41 and its purification, Protein Expression Purif. 33, 66-71.
22. 5 and cytochrome P450 reductase, J. Biol. Chem. 273, 17036-17049.
23. Kuzmic, P. (1996) Program DYNAFIT for the analysis of enzyme kinetic data: Application to HIV proteinase, Anal. Biochem. 237, 260-273.
24. I, T.-P., and Nancollas, G. H. (1972) EQUIL - A general computational method for the calculation of solution equilibria, Anal. Chem. 44, 1940-1950.
25. Price, K. V., Storm, R. M., and Lampinen, J. A. (2005) Differential Evolution - A Practical Approach to Global Optimization, Springer-Verlag, Berlin and Heidelberg.
26. Burnham, K. B., and Anderson, D. R. (2002) Model Selection and Multimodel Inference: A Practical Information-Theoretic Approach, 2nd ed., Springer-Verlag, New York.
27. Job, P. (1928) Recherches sur la formation de complexes mineraux en solution, et sur leur stabilité, Ann. Chim. (Paris) 9, 113-203.
28. Denbigh, K. (1964) The Principles of Chemical Equilibrium, Cambridge University Press, Cambridge.
29. Bates, D. M., and Watts, D. G. (1988) Nonlinear Regression Analysis and its Applications, Wiley, New York.
30. Kaminsky, L. S., Lee, J. J., and Guengerich, F. P. (1985) Interactions of cytochromes P-450 and NADPH-cytochrome P-450 reductase, in Abstracts, Fourth International Symposium on Comparative Biochemistry pp 43, Beerse, Belgium.
31. Kelley, R. W., Reed, J. R., and Backes, W. L. (2005) Effects of ionic strength on the functional interactions between CYP2B4 and CYP1A2, Biochemistry 44, 2632-2641.
32. Huang, C. Y. (1982) Determination of binding stoichiometry by the continuous variation method: the Job plot, Methods Enzymol. 87, 509-525.
33. Miwa, G. T., and Lu, A. Y. H. (1981) Studies on the stimulation of cytochrome P-450-dependent monooxygenase activity by dilauroylphosphatidylcholine, Arch. Biochem. Biophys. 211, 454-458.
34. Miwa, G. T., and Lu, A. Y. H. (1984) The association of cytochrome P-450 and NADPH-cytochrome P-450 reductase in phospholipid membranes, Arch. Biochem. Biophys. 234, 161-166.
35. Sevrukova, I. F., Kanaeva, I. P., Koen, Y. M., Samenkova, N. F., Bachmanova, G. I., and Archakov, A. I. (1994) Catalytic activity of cytochrome P4501A2 in reconstituted system with Emulgen 913, Arch. Biochem. Biophys. 311, 133-143.
36. Dean, W. L., and Gray, R. D. (1982) Relationship between state of aggregation and catalytic activity for cytochrome P-450LM2 and NADPH-cytochrome P-450 reductase, J. Biol. Chem. 257, 14679-14685.
37. Backes, W. L., and Kelley, R. W. (2003) Organization of multiple cytochrome P450s with NADPH-cytochrome P450 reductase in membranes, Pharmacol. Ther. 98, 221-233.
38. Hazai, E., and Kupfer, D. (2005) Interactions between Cyp2C9 and Cyp2C19 in reconstituted binary systems influence their catalytic activity: Possible rationale for the inability of Cyp2C19 to catalyze methoxychlor demethylation in human liver microsomes, Drug Metab. Dispos. 33, 157-164.
39. Davydov, D. R., Petushkova, N. A., Bobrovnikova, E. V., Knyushko, T. V., and Dansette, P. (2001) Association of cytochromes P450 1A2 and 2B4: are the interactions between different P450 species involved in the control of the monooxygenase activity and coupling?, Adv. Exp. Med. Biol. 500, 335-338.
40. Beechem, J. M. (1992) Global analysis of biochemical and biophysical data, Methods Enzymol. 210, 37-54.