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Volumn 67, Issue 8, 2007, Pages 987-998

L-type calcium channels mediate acetylcholine receptor aggregation on cultured muscle

Author keywords

Acetylcholine receptor; Aggregation; Agrin; Calcium; Cluster; Dysgenic mouse; Intracellular signaling; L type calcium channel; Muscle specific kinase; MuSK; Tyrosine phosphorylation

Indexed keywords

AGRIN; CALCIUM; CALCIUM CHANNEL BLOCKING AGENT; CALCIUM CHANNEL L TYPE; CHOLINERGIC RECEPTOR;

EID: 34548319238     PISSN: 19328451     EISSN: 1932846X     Source Type: Journal    
DOI: 10.1002/dneu.20397     Document Type: Article
Times cited : (7)

References (52)
  • 1
    • 0028172855 scopus 로고
    • Long-term synapse loss induced by focal blockade of postsynaptic receptors
    • Balice-Gordon RJ, Lichtman JW. 1994. Long-term synapse loss induced by focal blockade of postsynaptic receptors. Nature 372:519-524.
    • (1994) Nature , vol.372 , pp. 519-524
    • Balice-Gordon, R.J.1    Lichtman, J.W.2
  • 2
    • 0023910817 scopus 로고
    • Calcium currents in embryonic and neonatal mammalian skeletal muscle
    • Beam KG, Knudson CM. 1988a. Calcium currents in embryonic and neonatal mammalian skeletal muscle. J Gen Physiol 91:781-798.
    • (1988) J Gen Physiol , vol.91 , pp. 781-798
    • Beam, K.G.1    Knudson, C.M.2
  • 3
    • 0023913953 scopus 로고
    • Effect of postnatal development on calcium currents and slow charge movement in mammalian skeletal muscle
    • Beam KG, Knudson CM. 1988b. Effect of postnatal development on calcium currents and slow charge movement in mammalian skeletal muscle. J Gen Physiol 91:799-815.
    • (1988) J Gen Physiol , vol.91 , pp. 799-815
    • Beam, K.G.1    Knudson, C.M.2
  • 4
    • 0035795420 scopus 로고    scopus 로고
    • Agrin-induced phosphorylation of the acetylcholine receptor regulates cytoskeletal anchoring and clustering
    • Borges LS, Ferns M. 2001. Agrin-induced phosphorylation of the acetylcholine receptor regulates cytoskeletal anchoring and clustering. J Cell Biol 153:1-12.
    • (2001) J Cell Biol , vol.153 , pp. 1-12
    • Borges, L.S.1    Ferns, M.2
  • 5
    • 0036135033 scopus 로고    scopus 로고
    • Dual role for calcium in agrin signaling and acetylcholine receptor clustering
    • Borges LS, Lee Y, Ferns M. 2002. Dual role for calcium in agrin signaling and acetylcholine receptor clustering. J Neurobiol 50:69-79.
    • (2002) J Neurobiol , vol.50 , pp. 69-79
    • Borges, L.S.1    Lee, Y.2    Ferns, M.3
  • 6
    • 0042532329 scopus 로고    scopus 로고
    • Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition
    • Buffelli M, Burgess RW, Feng G, Lobe CG, Lichtman JW, Sanes JR. 2003. Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition. Nature 424:430-434.
    • (2003) Nature , vol.424 , pp. 430-434
    • Buffelli, M.1    Burgess, R.W.2    Feng, G.3    Lobe, C.G.4    Lichtman, J.W.5    Sanes, J.R.6
  • 7
    • 0036891517 scopus 로고    scopus 로고
    • Building the vertebrate neuromuscular synapse
    • Burden SJ. 2002. Building the vertebrate neuromuscular synapse. J Neurobiol 53:501-511.
    • (2002) J Neurobiol , vol.53 , pp. 501-511
    • Burden, S.J.1
  • 8
    • 0032538841 scopus 로고    scopus 로고
    • A functional role for specific spliced variants of the α7β1 integrin in acetylcholine receptor clustering
    • Burkin DJ, Gu M, Hodges BL, Campanelli JT, Kaufman SJ. 1998. A functional role for specific spliced variants of the α7β1 integrin in acetylcholine receptor clustering. J Cell Biol 143:1067-1075.
    • (1998) J Cell Biol , vol.143 , pp. 1067-1075
    • Burkin, D.J.1    Gu, M.2    Hodges, B.L.3    Campanelli, J.T.4    Kaufman, S.J.5
  • 9
    • 0033824947 scopus 로고    scopus 로고
    • Laminin and α7β1 integrin regulate agrin-induced clustering of acetylcholine receptors
    • Burkin DJ, Kim JE, Gu M, Kaufman SJ. 2000. Laminin and α7β1 integrin regulate agrin-induced clustering of acetylcholine receptors. J Cell Sci 113 (Part 16):2877-2886.
    • (2000) J Cell Sci , vol.113 , Issue.PART 16 , pp. 2877-2886
    • Burkin, D.J.1    Kim, J.E.2    Gu, M.3    Kaufman, S.J.4
  • 10
    • 0027462375 scopus 로고
    • Calcium influx and protein phosphorylation mediate the metabolic stabilization of synaptic acetylcholine receptors in muscle
    • Caroni P, Rotzler S, Britt JC, Brenner HR. 1993. Calcium influx and protein phosphorylation mediate the metabolic stabilization of synaptic acetylcholine receptors in muscle. J Neurosci 13:1315-1325.
    • (1993) J Neurosci , vol.13 , pp. 1315-1325
    • Caroni, P.1    Rotzler, S.2    Britt, J.C.3    Brenner, H.R.4
  • 11
    • 0027064887 scopus 로고
    • A single nucleotide deletion in the skeletal muscle-specific calcium channel transcript of muscular dysgenesis (mdg) mice
    • Chaudhari N. 1992. A single nucleotide deletion in the skeletal muscle-specific calcium channel transcript of muscular dysgenesis (mdg) mice. J Biol Chem 267:25636-25639.
    • (1992) J Biol Chem , vol.267 , pp. 25636-25639
    • Chaudhari, N.1
  • 13
    • 0023735183 scopus 로고
    • Verapamil, neuromuscular transmission and the nicotinic receptor
    • Edeson RO, Madsen BW, Milne RK, Le Dain AC. 1988. Verapamil, neuromuscular transmission and the nicotinic receptor. Eur J Pharmacol 151:301-306.
    • (1988) Eur J Pharmacol , vol.151 , pp. 301-306
    • Edeson, R.O.1    Madsen, B.W.2    Milne, R.K.3    Le Dain, A.C.4
  • 14
    • 0029928633 scopus 로고    scopus 로고
    • Agrin-induced acetylcholine receptor clustering in mammalian muscle requires tyrosine phosphorylation
    • Ferns M, Deiner M, Hall Z. 1996. Agrin-induced acetylcholine receptor clustering in mammalian muscle requires tyrosine phosphorylation. J Cell Biol 132:937-944.
    • (1996) J Cell Biol , vol.132 , pp. 937-944
    • Ferns, M.1    Deiner, M.2    Hall, Z.3
  • 15
    • 0038392756 scopus 로고    scopus 로고
    • Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction
    • Finn AJ, Feng G, Pendergast AM. 2003. Postsynaptic requirement for Abl kinases in assembly of the neuromuscular junction. Nat Neurosci 6:717-723.
    • (2003) Nat Neurosci , vol.6 , pp. 717-723
    • Finn, A.J.1    Feng, G.2    Pendergast, A.M.3
  • 16
    • 0030789733 scopus 로고    scopus 로고
    • Association of muscle-specific kinase MuSK with the acetylcholine receptor in mammalian muscle
    • Fuhrer C, Sugiyama JE, Taylor RG, Hall ZW. 1997. Association of muscle-specific kinase MuSK with the acetylcholine receptor in mammalian muscle. EMBO J 16:4951-4960.
    • (1997) EMBO J , vol.16 , pp. 4951-4960
    • Fuhrer, C.1    Sugiyama, J.E.2    Taylor, R.G.3    Hall, Z.W.4
  • 18
    • 0030806280 scopus 로고    scopus 로고
    • Kinase domain of the muscle-specific receptor tyrosine kinase (MuSK) is sufficient for phosphorylation but not clustering of acetylcholine receptors: Required role for the MuSK ectodomain?
    • Glass DJ, Apel ED, Shah S, Bowen DC, DeChiara TM, Stitt TN, Sanes JR, et al. 1997. Kinase domain of the muscle-specific receptor tyrosine kinase (MuSK) is sufficient for phosphorylation but not clustering of acetylcholine receptors: Required role for the MuSK ectodomain? Proc Natl Acad Sci USA 94:8848-8853.
    • (1997) Proc Natl Acad Sci USA , vol.94 , pp. 8848-8853
    • Glass, D.J.1    Apel, E.D.2    Shah, S.3    Bowen, D.C.4    DeChiara, T.M.5    Stitt, T.N.6    Sanes, J.R.7
  • 20
    • 25844510799 scopus 로고    scopus 로고
    • Alternative signaling pathways: When, where and why?
    • Gong Y, Zhang Z. 2005. Alternative signaling pathways: when, where and why? FEBS Lett 579:5265-5274.
    • (2005) FEBS Lett , vol.579 , pp. 5265-5274
    • Gong, Y.1    Zhang, Z.2
  • 21
    • 0032864614 scopus 로고    scopus 로고
    • Agrin-independent activation of the agrin signal transduction pathway
    • Grow WA, Ferns M, Gordon H. 1999a. Agrin-independent activation of the agrin signal transduction pathway. J Neurobiol 40:356-365.
    • (1999) J Neurobiol , vol.40 , pp. 356-365
    • Grow, W.A.1    Ferns, M.2    Gordon, H.3
  • 22
    • 0041382568 scopus 로고    scopus 로고
    • A mechanism for acetylcholine receptor clustering distinct from agrin signaling
    • Grow WA, Ferns M, Gordon H. 1999b. A mechanism for acetylcholine receptor clustering distinct from agrin signaling. Dev Neurosci 21:436-443.
    • (1999) Dev Neurosci , vol.21 , pp. 436-443
    • Grow, W.A.1    Ferns, M.2    Gordon, H.3
  • 23
    • 0032513058 scopus 로고    scopus 로고
    • Dimerization of the muscle-specific kinase induces tyrosine phosphorylation of acetylcholine receptors and their aggregation on the surface of myotubes
    • Hopf C, Hoch W. 1998. Dimerization of the muscle-specific kinase induces tyrosine phosphorylation of acetylcholine receptors and their aggregation on the surface of myotubes. J Biol Chem 273:6467-6473.
    • (1998) J Biol Chem , vol.273 , pp. 6467-6473
    • Hopf, C.1    Hoch, W.2
  • 24
    • 0028075105 scopus 로고
    • Depolarization-transcription signals in skeletal muscle use calcium flux through L channels, but bypass the sarcoplasmic reticulum
    • Huang CF, Flucher BE, Schmidt MM, Stroud SK, Schmidt J. 1994. Depolarization-transcription signals in skeletal muscle use calcium flux through L channels, but bypass the sarcoplasmic reticulum. Neuron 13:167-177.
    • (1994) Neuron , vol.13 , pp. 167-177
    • Huang, C.F.1    Flucher, B.E.2    Schmidt, M.M.3    Stroud, S.K.4    Schmidt, J.5
  • 25
    • 0034530293 scopus 로고    scopus 로고
    • Nitric oxide is a downstream mediator of agrin-induced acetylcholine receptor aggregation
    • Jones MA, Werle MJ. 2000. Nitric oxide is a downstream mediator of agrin-induced acetylcholine receptor aggregation. Mol Cell Neurosci 16:649-660.
    • (2000) Mol Cell Neurosci , vol.16 , pp. 649-660
    • Jones, M.A.1    Werle, M.J.2
  • 26
    • 0032537056 scopus 로고    scopus 로고
    • Glycine-receptor activation is required for receptor clustering in spinal neurons
    • Kirsch J, Betz H. 1998. Glycine-receptor activation is required for receptor clustering in spinal neurons. Nature 392:717-720.
    • (1998) Nature , vol.392 , pp. 717-720
    • Kirsch, J.1    Betz, H.2
  • 27
    • 0024498049 scopus 로고
    • Specific absence of the α 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis
    • Knudson CM, Chaudhari N, Sharp AH, Powell JA, Beam KG, Campbell KP. 1989. Specific absence of the α 1 subunit of the dihydropyridine receptor in mice with muscular dysgenesis. J Biol Chem 264:1345-1348.
    • (1989) J Biol Chem , vol.264 , pp. 1345-1348
    • Knudson, C.M.1    Chaudhari, N.2    Sharp, A.H.3    Powell, J.A.4    Beam, K.G.5    Campbell, K.P.6
  • 28
    • 0001634869 scopus 로고    scopus 로고
    • Calreticulin couples calcium release and calcium influx in integrin-mediated calcium signaling
    • Kwon MS, Park CS, Choi K, Ahnn J, Kim JI, Eom SH, Kaufman SJ, et al. 2000. Calreticulin couples calcium release and calcium influx in integrin-mediated calcium signaling. Mol Biol Cell 11:1433-1443.
    • (2000) Mol Biol Cell , vol.11 , pp. 1433-1443
    • Kwon, M.S.1    Park, C.S.2    Choi, K.3    Ahnn, J.4    Kim, J.I.5    Eom, S.H.6    Kaufman, S.J.7
  • 29
    • 0035953645 scopus 로고    scopus 로고
    • Distinct roles of nerve and muscle in postsynaptic differentiation of the neuromuscular synapse
    • Lin W, Burgess RW, Dominguez B, Pfaff SL, Sanes JR, Lee KF. 2001. Distinct roles of nerve and muscle in postsynaptic differentiation of the neuromuscular synapse. Nature 410:1057-1064.
    • (2001) Nature , vol.410 , pp. 1057-1064
    • Lin, W.1    Burgess, R.W.2    Dominguez, B.3    Pfaff, S.L.4    Sanes, J.R.5    Lee, K.F.6
  • 31
    • 0037182593 scopus 로고    scopus 로고
    • Laminin-1 redistributes postsynaptic proteins and requires rapsyn, tyrosine phosphorylation, and Src and Fyn to stably cluster acetylcholine receptors
    • Marangi PA, Wieland ST, Fuhrer C. 2002. Laminin-1 redistributes postsynaptic proteins and requires rapsyn, tyrosine phosphorylation, and Src and Fyn to stably cluster acetylcholine receptors. J Cell Biol 157:883-895.
    • (2002) J Cell Biol , vol.157 , pp. 883-895
    • Marangi, P.A.1    Wieland, S.T.2    Fuhrer, C.3
  • 32
    • 0031972621 scopus 로고    scopus 로고
    • Intracellular calcium regulates agrin-induced acetylcholine receptor clustering
    • Megeath LJ, Fallon JR. 1998. Intracellular calcium regulates agrin-induced acetylcholine receptor clustering. J Neurosci 18:672-678.
    • (1998) J Neurosci , vol.18 , pp. 672-678
    • Megeath, L.J.1    Fallon, J.R.2
  • 33
    • 0242569304 scopus 로고    scopus 로고
    • Calcium-dependent maintenance of agrin-induced postsynaptic specializations
    • Megeath LJ, Kirber MT, Hopf C, Hoch W, Fallon JR. 2003. Calcium-dependent maintenance of agrin-induced postsynaptic specializations. Neuroscience 122:659-668.
    • (2003) Neuroscience , vol.122 , pp. 659-668
    • Megeath, L.J.1    Kirber, M.T.2    Hopf, C.3    Hoch, W.4    Fallon, J.R.5
  • 34
    • 0034004905 scopus 로고    scopus 로고
    • Critical dependence of cAMP response element-binding protein phosphorylation on L-type calcium channels supports a selective response to EPSPs in preference to action potentials
    • Mermelstein PG, Bito H, Deisseroth K, Tsien RW. 2000. Critical dependence of cAMP response element-binding protein phosphorylation on L-type calcium channels supports a selective response to EPSPs in preference to action potentials. J Neurosci 20:266-273.
    • (2000) J Neurosci , vol.20 , pp. 266-273
    • Mermelstein, P.G.1    Bito, H.2    Deisseroth, K.3    Tsien, R.W.4
  • 35
    • 0019257264 scopus 로고
    • Receptor stability and channel conversion in the subsynaptic membrane of the developing mammalian neuromuscular junction
    • Michler A, Sakmann B. 1980. Receptor stability and channel conversion in the subsynaptic membrane of the developing mammalian neuromuscular junction. Dev Biol 80:1-17.
    • (1980) Dev Biol , vol.80 , pp. 1-17
    • Michler, A.1    Sakmann, B.2
  • 36
    • 0035370294 scopus 로고    scopus 로고
    • Src-class kinases act within the agrin/MuSK pathway to regulate acetylcholine receptor phosphorylation, cytoskeletal anchoring, and clustering
    • Mohamed AS, Rivas-Plata KA, Kraas JR, Saleh SM, Swope SL. 2001. Src-class kinases act within the agrin/MuSK pathway to regulate acetylcholine receptor phosphorylation, cytoskeletal anchoring, and clustering. J Neurosci 21:3806-3818.
    • (2001) J Neurosci , vol.21 , pp. 3806-3818
    • Mohamed, A.S.1    Rivas-Plata, K.A.2    Kraas, J.R.3    Saleh, S.M.4    Swope, S.L.5
  • 37
    • 0032519864 scopus 로고    scopus 로고
    • Laminin and α-dystroglycan mediate acetylcholine receptor aggregation via a MuSK-independent pathway
    • Montanaro F, Gee SH, Jacobson C, Lindenbaum MH, Froehner SC, Carbonetto S. 1998. Laminin and α-dystroglycan mediate acetylcholine receptor aggregation via a MuSK-independent pathway. J Neurosci 18:1250-1260.
    • (1998) J Neurosci , vol.18 , pp. 1250-1260
    • Montanaro, F.1    Gee, S.H.2    Jacobson, C.3    Lindenbaum, M.H.4    Froehner, S.C.5    Carbonetto, S.6
  • 38
    • 0347124688 scopus 로고    scopus 로고
    • Targeting of recombinant agrin to axonal growth cones
    • Neuhuber B, Daniels MP. 2003. Targeting of recombinant agrin to axonal growth cones. Mol Cell Neurosci 24:1180-1196.
    • (2003) Mol Cell Neurosci , vol.24 , pp. 1180-1196
    • Neuhuber, B.1    Daniels, M.P.2
  • 39
    • 0020325288 scopus 로고
    • Extensive multiple innervation and abnormal synaptogenesis in muscular dysgenesis (mdg/mdg) in the mouse embryo
    • Pincon-Raymond M, Rieger F. 1982. Extensive multiple innervation and abnormal synaptogenesis in muscular dysgenesis (mdg/mdg) in the mouse embryo. Reprod Nutr Dev 22:217-226.
    • (1982) Reprod Nutr Dev , vol.22 , pp. 217-226
    • Pincon-Raymond, M.1    Rieger, F.2
  • 40
    • 0021340228 scopus 로고
    • Distribution and quantification of ACh receptors and innervation in diaphragm muscle of normal and mdg mouse embryos
    • Powell JA, Rieger F, Blondet B, Dreyfus P, Pincon-Raymond M. 1984. Distribution and quantification of ACh receptors and innervation in diaphragm muscle of normal and mdg mouse embryos. Dev Biol 101:168-180.
    • (1984) Dev Biol , vol.101 , pp. 168-180
    • Powell, J.A.1    Rieger, F.2    Blondet, B.3    Dreyfus, P.4    Pincon-Raymond, M.5
  • 41
    • 0028052235 scopus 로고
    • Comparison of innervation and agrin-induced tyrosine phosphorylation of the nicotinic acetylcholine receptor
    • Qu Z, Huganir RL. 1994. Comparison of innervation and agrin-induced tyrosine phosphorylation of the nicotinic acetylcholine receptor. J Neurosci 14:6834-6841.
    • (1994) J Neurosci , vol.14 , pp. 6834-6841
    • Qu, Z.1    Huganir, R.L.2
  • 42
    • 0026633248 scopus 로고
    • Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions
    • Reist NE, Werle MJ, McMahan UJ. 1992. Agrin released by motor neurons induces the aggregation of acetylcholine receptors at neuromuscular junctions. Neuron 8:865-868.
    • (1992) Neuron , vol.8 , pp. 865-868
    • Reist, N.E.1    Werle, M.J.2    McMahan, U.J.3
  • 44
    • 0022347334 scopus 로고
    • Nicotinic acetylcholine receptors in vertebrate muscle: Properties, distribution and neural control
    • Salpeter MM, Loring RH. 1985. Nicotinic acetylcholine receptors in vertebrate muscle: Properties, distribution and neural control. Prog Neurobiol 25:297-325.
    • (1985) Prog Neurobiol , vol.25 , pp. 297-325
    • Salpeter, M.M.1    Loring, R.H.2
  • 45
    • 0023749290 scopus 로고
    • Distribution of extrajunctional acetylcholine receptors on a vertebrate muscle: Evaluated by using a scanning electron microscope autoradiographic procedure
    • Salpeter MM, Marchaterre M, Harris R. 1988. Distribution of extrajunctional acetylcholine receptors on a vertebrate muscle: Evaluated by using a scanning electron microscope autoradiographic procedure. J Cell Biol 106:2087-2093.
    • (1988) J Cell Biol , vol.106 , pp. 2087-2093
    • Salpeter, M.M.1    Marchaterre, M.2    Harris, R.3
  • 46
    • 0037413907 scopus 로고    scopus 로고
    • Lithium inhibits a late step in agrin-induced AChR aggregation
    • Sharma SK, Wallace BG. 2003. Lithium inhibits a late step in agrin-induced AChR aggregation. J Neurobiol 54:346-357.
    • (2003) J Neurobiol , vol.54 , pp. 346-357
    • Sharma, S.K.1    Wallace, B.G.2
  • 47
    • 0030879133 scopus 로고    scopus 로고
    • Laminin-induced acetylcholine receptor clustering: An alternative pathway
    • Sugiyama JE, Glass DJ, Yancopoulos GD, Hall ZW. 1997. Laminin-induced acetylcholine receptor clustering: An alternative pathway. J Cell Biol 139:181-191.
    • (1997) J Cell Biol , vol.139 , pp. 181-191
    • Sugiyama, J.E.1    Glass, D.J.2    Yancopoulos, G.D.3    Hall, Z.W.4
  • 48
    • 0020627859 scopus 로고
    • Laminin induces acetylcholine receptor aggregation on cultured myotubes and enhances the receptor aggregation activity of a neuronal factor
    • Vogel Z, Christian CN, Vigny M, Bauer HC, Sonderegger P, Daniels MP. 1983. Laminin induces acetylcholine receptor aggregation on cultured myotubes and enhances the receptor aggregation activity of a neuronal factor. J Neurosci 3:1058-1068.
    • (1983) J Neurosci , vol.3 , pp. 1058-1068
    • Vogel, Z.1    Christian, C.N.2    Vigny, M.3    Bauer, H.C.4    Sonderegger, P.5    Daniels, M.P.6
  • 49
    • 0023729036 scopus 로고
    • ++ and phorbol ester
    • ++ and phorbol ester. J Cell Biol 107:267-278.
    • (1988) J Cell Biol , vol.107 , pp. 267-278
    • Wallace, B.G.1
  • 50
    • 0026684767 scopus 로고
    • Mechanism of agrin-induced acetylcholine receptor aggregation
    • Wallace BG. 1992. Mechanism of agrin-induced acetylcholine receptor aggregation. J Neurobiol 23:592-604.
    • (1992) J Neurobiol , vol.23 , pp. 592-604
    • Wallace, B.G.1
  • 51
    • 0025779156 scopus 로고
    • Agrin induces phosphorylation of the nicotinic acetylcholine receptor
    • Wallace BG, Qu Z, Huganir RL. 1991. Agrin induces phosphorylation of the nicotinic acetylcholine receptor. Neuron 6:869-878.
    • (1991) Neuron , vol.6 , pp. 869-878
    • Wallace, B.G.1    Qu, Z.2    Huganir, R.L.3
  • 52
    • 0017759258 scopus 로고
    • Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle
    • Yaffe D, Saxel O. 1977. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270:725-727.
    • (1977) Nature , vol.270 , pp. 725-727
    • Yaffe, D.1    Saxel, O.2


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