Optimization of short hairpin RNA for lentiviral-mediated RNAi against WAS

Biochemical and Biophysical Research Communications

Volumn 362, Issue 2, 2007, Pages 498-503

  Jeanson Leh, Laurence ^a   Blondeau, Johanna ^a   Galy, Anne ^a  

^a INSERM   (France)

Author keywords

Lentiviral vectors; RNAi; shRNA; Wiskott Aldrich syndrome

Indexed keywords

NEURAL WISKOTT ALDRICH SYNDROME PROTEIN; SHORT HAIRPIN RNA; VIRUS VECTOR;

ARTICLE; CD34 SELECTION; CONTROLLED STUDY; GENE EXPRESSION; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; LENTIVIRINAE; PRIORITY JOURNAL; REPORTER GENE; RNA INTERFERENCE; RNA SEQUENCE;

2',5'-OLIGOADENYLATE SYNTHETASE; BASE SEQUENCE; BLOTTING, WESTERN; CELL LINE; CELL LINE, TUMOR; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HCT116 CELLS; HUMANS; LENTIVIRUS; NUCLEIC ACID CONFORMATION; RECOMBINANT FUSION PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANSCRIPTION FACTOR TFIID; TRANSFECTION; WISKOTT-ALDRICH SYNDROME PROTEIN;

EID: 34548299760     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2007.08.013     Document Type: Article

References (22)

1. Kim D.H., and Rossi J.J. Strategies for silencing human disease using RNA interference. Nat. Rev. Genet. 8 (2007) 173-184
2. Amarzguioui M., Rossi J.J., and Kim D. Approaches for chemically synthesized siRNA and vector-mediated RNAi. FEBS Lett. 579 (2005) 5974-5981
3. Bartel D.P. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116 (2004) 281-297
4. Grimm D., Streetz K.L., Jopling C.L., Storm T.A., Pandey K., Davis C.R., Marion P., Salazar F., and Kay M.A. Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 441 (2006) 537-541
5. Yi R., Doehle B.P., Qin Y., Macara I.G., and Cullen B.R. Overexpression of exportin 5 enhances RNA interference mediated by short hairpin RNAs and microRNAs. Rna 11 (2005) 220-226
6. Olivier A., Jeanson-Leh L., Bouma G., Compagno D., Blondeau J., Seye K., Charrier S., Burns S., Thrasher A.J., Danos O., Vainchenker W., and Galy A. A partial down-regulation of WASP is sufficient to inhibit podosome formation in dendritic cells. Mol. Ther. (2005)
7. Gregory R.I., Chendrimada T.P., Cooch N., and Shiekhattar R. Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. Cell 123 (2005) 631-640
8. Kim D.H., Behlke M.A., Rose S.D., Chang M.S., Choi S., and Rossi J.J. Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy. Nat. Biotechnol. 23 (2005) 222-226
9. Siolas D., Lerner C., Burchard J., Ge W., Linsley P.S., Paddison P.J., Hannon G.J., and Cleary M.A. Synthetic shRNAs as potent RNAi triggers. Nat. Biotechnol. 23 (2005) 227-231
10. Boden D., Pusch O., Silbermann R., Lee F., Tucker L., and Ramratnam B. Enhanced gene silencing of HIV-1 specific siRNA using microRNA designed hairpins. Nucleic Acids Res. 32 (2004) 1154-1158
11. Brummelkamp T.R., Bernards R., and Agami R. A system for stable expression of short interfering RNAs in mammalian cells. Science 296 (2002) 550-553
12. Fish R.J., and Kruithof E.K. Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. BMC Mol. Biol. 5 (2004) 9
13. Bridge A.J., Pebernard S., Ducraux A., Nicoulaz A.L., and Iggo R. Induction of an interferon response by RNAi vectors in mammalian cells. Nat. Genet. 34 (2003) 263-264
14. Sun Y., and Leaman D.W. Involvement of Noxa in cellular apoptotic responses to interferon, double-stranded RNA, and virus infection. J. Biol. Chem. 280 (2005) 15561-15568
15. Jacque J.M., Triques K., and Stevenson M. Modulation of HIV-1 replication by RNA interference. Nature 418 (2002) 435-438
16. Paddison P.J., Caudy A.A., Bernstein E., Hannon G.J., and Conklin D.S. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16 (2002) 948-958
17. Yu J.Y., DeRuiter S.L., and Turner D.L. RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells. Proc. Natl. Acad. Sci. USA 99 (2002) 6047-6052
18. Gonzalez S., Castanotto D., Li H., Olivares S., Jensen M.C., Forman S.J., Rossi J.J., and Cooper L.J. Amplification of RNAi-targeting HLA mRNAs. Mol. Ther. 11 (2005) 811-818
19. Chang K., Elledge S.J., and Hannon G.J. Lessons from Nature: microRNA-based shRNA libraries. Nat. Methods 3 (2006) 707-714
20. Dickins R.A., Hemann M.T., Zilfou J.T., Simpson D.R., Ibarra I., Hannon G.J., and Lowe S.W. Probing tumor phenotypes using stable and regulated synthetic microRNA precursors. Nat. Genet. 37 (2005) 1289-1295
21. Stegmeier F., Hu G., Rickles R.J., Hannon G.J., and Elledge S.J. A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells. Proc. Natl. Acad. Sci. USA 102 (2005) 13212-13217
22. -/- mice: functional inactivation of p53 in developing T cells. Blood 104 (2004) 3886-3893