Characterization of spectral FRET imaging microscopy for monitoring nuclear protein interactions

Journal of Microscopy

Volumn 228, Issue 2, 2007, Pages 139-152

  Chen, Ye ^a   Mauldin, Joshua P ^b   Day, Richard N ^b   Periasamy, Ammasi ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

^b UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

C EBP ; Confocal; FRET; Green fluorescent proteins; Protein dimerization; Spectral bleedthrough; Spectral imaging

Indexed keywords

DIMERIZATION; ENERGY TRANSFER; TRANSCRIPTION; TRANSCRIPTION FACTORS;

BLEED-THROUGH; C/EBPΑ; CONFOCAL; GREEN FLUORESCENT PROTEIN; IMAGING MICROSCOPY; LIVING CELL; PROTEIN DIMERIZATION; SPECTRA'S; SPECTRAL BLEEDTHROUGH; SPECTRAL IMAGING;

FORSTER RESONANCE ENERGY TRANSFER;

AMINO ACID; GREEN FLUORESCENT PROTEIN; TRANSCRIPTION FACTOR;

ALGORITHM; ARTICLE; CELL NUCLEUS; COMPUTER PROGRAM; DIMERIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGE ANALYSIS; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION;

EID: 34548272472     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2007.01838.x     Document Type: Article

References (49)

1. Bastiaens, P.I. & Jovin, T.M. (1996) Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C beta I. Proc. Natl. Acad. Sci. USA 93, 8407 8412.
2. Berney, C. & Danuser, G. (2003) FRET or no FRET: a quantitative comparison. Biophys. J. 84, 3992 4010.
3. Centonze, V.E., Sun, M., Masuda, A., Gerritsen, H. & Herman, B. (2003) Fluorescence resonance energy transfer imaging microscopy. Meth. Enzymol. 360, 542 560.
4. Chen, Y., Mills, J.D. & Periasamy, A. (2003) Protein interactions in cells and tissues using FLIM and FRET. Differentiation 71, 528 541.
5. Chen, Y. & Periasamy, A. (2004) Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization. Microsc. Res. Tech. 63, 72 80.
6. Chen, Y., Elangovan, M. & Periasamy, A. (2005) FRET data analysis - the algorithm. Molecular Imaging: FRET Microscopy and Spectroscopy (ed. by A. Periasamy and R.N. Day pp. 126 145. Academic-Elsevier Press, New York.
7. Chen, Y. & Periasamy, A. (2006) Intensity range based quantitative FRET data analysis to localize the protein molecules in living cell nucleus. J. Fluoresc. 16, 95 104.
8. Chudakov, D.M., Lukyanov, S. & Lukyanov, K.A. (2005) Fluorescent proteins as a toolkit for in vivo imaging. Trends Biotechnol. 23, 605 613.
9. Clegg, R. 1992. Fluorescence resonance energy transfer and nucleic acids. Meth. Enzymol. 211, 353 388.
10. Clegg, R.M., Holub, O. & Gohlke, C. (2003) Fluorescence lifetime-resolved imaging: measuring lifetimes in an image. Meth. Enzymol. 360, 509 542.
11. Craig, J.M., Earnshaw, W.C. & Vagnarelli, P. (1999) Mammalian centromeres: DNA sequence, protein composition, and role in cell cycle progression. Exp. Cell Res. 246, 249 262.
12. Day, R.N., Periasamy, A. & Schaufele, F. (2001) Fluorescence resonance energy transfer microscopy of localized protein interactions in the living cell nucleus. Methods 25, 4 18.
13. Day, R.N., Voss, T.C., Enwright, III., J.F., Booker, C.F., Periasamy, A. & Schaufels, F. (2003) Imaging the localized protein interactions between Pit-1 and the CCAAT/enhancer binding protein alpha (C/EBPa) in the living pituitary cell nucleus. Mol. Endo. 17, 333 345.
14. Day, R.N. & Schaufele, F. (2005) Molecular interactions in living cells. Mol. Endocrinol. 19, 1675 1686.
15. Demarco, I.A., Voss, T.C., Booker, C.F. & Day, R.N. (2006) Dynamic interactions between Pit-1 and C/EBPalpha in the pituitary cell nucleus. Mol. Cell Biol. 26, 8087 8098.
16. Dickinson, M.E., Simbuerger, E., Zimmermann, B., Waters, C.W. & Fraser, S.E. (2003) Multiphoton excitation spectra in biological samples. J. Biomed. Opt. 8, 329 338.
17. Dong, C.Y., French, T., So, P.T., Buehler, C., Berland, K.M. & Gratton, E. (2003) Fluorescence-lifetime imaging techniques for microscopy. Methods Cell Biol. 72, 431 464.
18. Elangovan, M., Wallrabe, H., Chen, Y., Day, R.N., Barroso, M. & Periasamy, A. (2003) Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy. Methods 29, 58 73.
19. Enwright, J.F., III., Kawecki-Crook, M.A., Voss, T.C., Schaufele, F. & Day, R.N. (2003) A PIT-1 homeodomain mutant blocks the intranuclear recruitment of the CCAAT/enhancer binding protein alpha required for prolactin gene transcription. Mol. Endocrinol. 17, 209 222.
20. Gordon, G.W., Berry, G., Liang, X.H., Levine, B. & Herman, B. (1998) Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys. J. 74, 2702 2713.
21. Jares-Erijman, E.A. & Jovin, T.M. (2003) FRET imaging. Nat. Biotechnol. 21, 1387 1395.
22. Johnson, P.F. (2005) Molecular stop signs: regulation of cell-cycle arrest by C/EBP transcription factors. J. Cell Sci. 118, 2545 2555.
23. Koushik, S.V., Chen, H., Thaler, C., Puhl, III., H.L. & Vogel, S.S. (2006) Cerulean, Venus, and Venusy67c FRET reference standards. Biophys. J. 91, L99 L101.
24. Lakowicz, J.R. (1999) Principles of Fluorescence Spectroscopy, 2nd edn. Plenum, New York.
25. Lew, D., Brady, H., Klausing, K., Yaginuma, K., Theill, L.E., Stauber, C., Karin, M. & Mellon, P.L. (1992) GHF-1-promoter-targeted immortalization of a somatotropic progenitor cell results in dwarfism in transgenic mice. Genes Dev. 7, 683 693.
26. Nagai, T., Ibata, K., Park, E.S., Kubota, M., Mikoshiba, K. & Miyawaki, A. (2002) A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat. Biotechnol. 20, 87 90.
27. Neher, R.A. & Neher, E. (2004a) Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. J. Microsc. 213, 46 62.
28. Neher, R.A. & Neher, E. (2004b) Applying spectral fingerprinting to the analysis of FRET images. Microsc. Res. Tech. 64, 185 195.
29. Park, Y.C., Burkitt, V., Villa, A.R., Tong, L. & Wu, H. (1999) Structural basis for self-association and receptor recognition of human TRAF2. Nature 398, 533 538.
30. Periasamy, A. & Day, R.N. (2005) Molecular Imaging: FRET Microscopy and Spectroscopy. Academic-Elsevier Press, New York.
31. Perrod, S. & Gasser, S.M. (2003) Long-range silencing and position effects at telomeres and centromeres: parallels and differences. Cell Mol. Life Sci. 60, 2303 2318.
32. Rizzo, M.A., Springer, G.H., Granada, B. & Piston, D.W. (2004) An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22, 445 449.
33. Schaufele, F., Enwright, J.F. III., Wang, X., Teoh, C., Srihari, R., Erickson, R., MacDougald, O.A. & Day, R.N. (2001) CCAAT/enhancer binding protein alpha assembles essential cooperating factors in common subnuclear domains. Mol. Endocrinol. 15, 1665 1676.
34. Shaner, N.C., Steinbach, P.A. & Tsien, R.Y. (2005) A guide to choosing fluorescent proteins. Nat. Methods 2, 905 909.
35. Suhling, K., Siegel, J., Phillips, D., French, P.M., Leveque-Fort, S., Webb, S.E. & Davis, D.M. (2002) Imaging the environment of green fluorescent protein. Biophys. J. 83, 3589 3595.
36. Tang, Q.Q. & Lane, M.D. (1999) Activation and centromeric localization of CCAAT/enhancer-binding proteins during the mitotic clonal expansion of adipocyte differentiation. Genes Dev. 13, 2231 2241.
37. Thaler, C., Koushik, S.V., Blank, P.S. & Vogel, S.S. (2005) Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer. Biophys. J. 89, 2736 2749.
38. Tramier, M., Kemnitz, K., Durieux, C. & Coppey-Moisan, M. (2004) Picosecond time-resolved microspectrofluorometry in live cells exemplified by complex fluorescence dynamics of popular probes ethidium and cyan fluorescent protein. J. Microsc. 213, 110 118.
39. Vig, B.K. & Willcourt, M. (1998) Decondensation of pericentric heterochromatin alters the sequence of centromere separation in mouse cells. Chromosoma 107, 417 423.
40. Vinson, C.R., Sigler, P.B. & McKnight, S.L. (1989) Scissors-grip model for DNA recognition by a family of leucine zipper proteins. Science 246, 911 916.
41. Vogel, S.S., Thaler, C. & Koushik, S.V. (2006) Fanciful FRET. Sci. STKE, re2 http://www.stke.org/).
42. Wallrabe, H., Elangovan, M., Burchard, A., Periasamy, A. & Barroso, M. (2003) Confocal FRET microscopy to measure clustering of ligand-receptor complexes in endocytic membranes. Biophys. J. 85, 559 571.
43. Wallrabe, H. & Periasamy, A. (2005) FRET-FLIM microscopy and spectroscopy in the biomedical sciences. Curr. Opin. Biotech. 16, 19 27.
44. Wallrabe, H., Chen, Y., Periasamy, A. & Barroso, M. (2006) Issues in confocal microscopy for quantitative FRET analysis. Microsc. Res. Tech. 69, 196 206.
45. Wouters, F.S., Bastiaens, P.I., Wirtz, K.W. & Jovin, T.M. (1998) FRET Microscopy demonstrates molecular association of non-specific lipid transfer protein (nsL-TP) with fatty acid oxidation enzymes in peroxisomes. EMBO J. 17, 7179 7189.
46. Xia, Z. & XLiu, T.M. (2001) Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes. Biophys. J. 81, 2395 2402.
47. Zacharias, D.A., Violin, J.D., Newton, A.C. & Tsien, R.Y. (2002) Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296, 913 916.
48. Zal, T. & Gascoigne, N.R. (2004) Photobleaching-corrected FRET efficiency imaging of live cells. Biophys. J. 86, 3923 3939.
49. Zimmermann, T., Rietdorf, J., Girod, A., Georget, V. & Pepperkok, R. (2002) Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair. FEBS Lett. 531, 245 249.