FRET microscopy demonstrates molecular association of non-specific lipid transfer protein (nsL-TP) with fatty acid oxidation enzymes in peroxisomes

EMBO Journal

Volumn 17, Issue 24, 1998, Pages 7179-7189

  Wouters, Fred S ^a,b,c   Bastiaens, Philippe I H ^b,c   Wirtz, Karel W A ^a   Jovin, Thomas M ^b  

^a UTRECHT UNIVERSITY   (Netherlands)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

^c IMPERIAL CANCER RESEARCH FUND   (United Kingdom)

Author keywords

3 ketoacyl CoA thiolase; Acyl CoA oxidase; Fluorescence resonance energy transfer; PMP70; Sterol carrier protein 2

Indexed keywords

ACETYL COENZYME A ACYLTRANSFERASE; ACYL COENZYME A OXIDASE; ANTIBODY; CATALASE; CYSTEINE; LEUCINE; LIPID TRANSFER PROTEIN;

ANIMAL CELL; ARTICLE; BLEACHING; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENERGY TRANSFER; FATTY ACID OXIDATION; FIBROBLAST CULTURE; FLUORESCENCE MICROSCOPY; IMMUNOFLUORESCENCE; MICROINJECTION; MOUSE; NONHUMAN; PEROXISOME; PRIORITY JOURNAL;

3T3 CELLS; ACETYL-COA C-ACYLTRANSFERASE; ACYL-COA OXIDASE; ANIMALS; ATP-BINDING CASSETTE TRANSPORTERS; BIOLOGICAL TRANSPORT; CARRIER PROTEINS; CATALASE; CELL COMPARTMENTATION; CHROMATOGRAPHY, AFFINITY; ENERGY TRANSFER; FATTY ACIDS; IMAGE PROCESSING, COMPUTER-ASSISTED; MEMBRANE PROTEINS; MICE; MICE, INBRED BALB C; MICROBODIES; MICROSCOPY; MULTIENZYME COMPLEXES; OXIDOREDUCTASES; PLANT PROTEINS; PROTEIN BINDING;

ANIMALIA;

EID: 0032535138     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.24.7179     Document Type: Article

References (18)

1. Antonenkov,V.D., Van Veldhoven,P.P., Waelkens,E. and Mannerts,G.P. (1997) Substrate specificities of 3-oxoacyl-CoA thiolase A and sterol carrier protein 2/3-oxoacyl-CoA thiolase purified from normal rat liver peroxisomes. Sterol carrier protein 2/3-oxoacyl-CoA thiolase is involved in the metabolism of 2-methyl-branched fatty acids and bile acid intermediates. J. Biol. Chem., 272, 26023-26031.
2. Baker,M.E., Billheimer,J.T. and Strauss,J.F.,III (1991) Similarity between the amino-terminal portion of mammalian 58-kD sterol carrier protein (SCPx) and Escherichia coli acetyl-CoA acyltransferase: evidence for a gene fusion in SCPx. DNA Cell Biol., 10, 695-698.
3. Bastiaens,P.I.H. and Jovin,T.M. (1996) Microspectroscopic imaging tracks the intracellular processing of a signal transduction protein: fluorescent-labeled protein kinase C β I. Proc. Natl Acad. Sci. USA, 93, 8407-8412.
4. Bastiaens,P.I.H. and Jovin,T.M. (1998) Fluorescence resonance energy transfer microscopy. In Celis,J.E. (ed.), Cell Biology: A Laboratory Handbook. 2nd edn, Vol. 3. Academic Press, New York, NY, pp. 136-146.
5. Bastiaens,P.I.H., DeBeus,A., Lacker,M., Somerharju,P., Vauhkonen,M. and Eisinger,J. (1990) Resonance energy transfer from a cylindrical distribution of donors to a plane of acceptors. Location of apo-B100 protein on the human low-density lipoprotein particle. Biophys. J., 58, 665-675.
6. Bastiaens,P.I.H., Majoul,I.V., Verveer,P.J., Soeling,H.D. and Jovin,T.M. (1996) Imaging the intracellular trafficking and state of the AB5 quaternary structure of cholera toxin. EMBO J., 15, 4246-4253.
7. Billheimer,J.T. and Reinhart,M.P. (1990) Intracellular trafficking of sterols. Subcell. Biochem., 16, 301-331.
8. Billheimer,J.T., Strehl,L.L., Strauss,J.F., III and Davis,L.G. (1990) Characterization of a cDNA encoding rat sterol carrier protein 2. DNA Cell Biol., 9, 159-165.
9. Bloj,B. and Zilversmit,D.B. (1977) Rat liver proteins capable of transferring phosphatidylethanolamine. Purification and transfer activity for other phospholipids and cholesterol. J. Biol. Chem., 252, 1613-1619.
10. Bloj,B. and Zilversmit,D.B. (1981) Accelerated transfer of neutral glycosphingolipids and ganglioside GM1 by a purified lipid transfer protein. J. Biol Chem., 256, 5988-5991.
11. Bordier,C. (1981) Phase separation of integral membrane proteins in Triton X-I14 solution. J. Biol. Chem., 256, 1604-1607.
12. Ceolotto,C., Flekl,W., Schorsch,F.J., Tahotna,D., Hapala,I., Hrastnik,C., Paltauf,F. and Daum,G. (1996) Characterization of a non-specific lipid transfer protein associated with the peroxisomal membrane of the yeast, Saccharomyces cerevisiae. Biochim. Biophys. Acta, 1285, 71-78.
13. Chanderbhan,R., Noland,B.J., Scallen,T.J. and Vahouny,G.V. (1982) Sterol carrier protein-2. Delivery of cholesterol from adrenal lipid droplets to mitochondria for pregnenolone synthesis. J. Biol. Chem., 257, 8928-8934.
14. Crain,R.C. and Zilversmit,D.B. (1980) Two nonspecific phospholipid exchange proteins from beef liver. I. Purification and characterization. Biochemistry; 19, 1433-1439.
15. Frolov,A., Cho,T.H., Billheimer,J.T. and Schroeder,F. (1996) Sterol carrier protein-2, a new fatty acyl coenzyme A-binding protein. J. Biol. Chem., 271, 31878-31884.
16. Fujiki,Y., Tsuneoka,M. and Tashiro,Y. (1989) Biosynthesis of nonspecific lipid transfer protein (sterol carrier protein 2) on free polyribosomes as a larger precursor in rat liver. J. Biochem., 106, 1126-1131.
17. GadelIa,T.W.J.,Jr and Jovin,T.M. (1995) Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A. stereochemical model for tyrosine kinase receptor activation. J. Cell Biol., 129, 1543-1558.
18. Gadella,T.W.J.,Jr and Jovin,T.M. (1997) Fast algorithms for the analysis of single and double exponential decay curves with a background term. Application to time-resolved imaging microscopy. Bioimaging, 5, 5-39.