Uranium induces apoptosis and is genotoxic to normal rat kidney (NRK-52E) proximal cells

Toxicological Sciences

Volumn 98, Issue 2, 2007, Pages 479-487

  Thiébault, Céline ^a   Carrière, Marie ^a   Milgram, Sarah ^a   Simon, Angélique ^a   Avoscan, Laure ^a   Gouget, Barbara ^a  

^a CNRS   (France)

Author keywords

Apoptosis; Comet assay; DNA damage; Genotoxicity; Renal cells; Uranium

Indexed keywords

CASPASE 10; CASPASE 8; CASPASE 9; DOUBLE STRANDED DNA; URANIUM;

ANIMAL CELL; APOPTOSIS; ARTICLE; CHROMOSOME BREAKAGE; CHROMOSOME LOSS; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; DNA STRAND BREAKAGE; GENOTOXICITY; IMMUNOHISTOCHEMISTRY; KIDNEY FAILURE; MEMBRANE POTENTIAL; MICRONUCLEUS TEST; MITOCHONDRIAL MEMBRANE; NONHUMAN; RAT; TOXICITY TESTING;

ANIMALS; APOPTOSIS; CASPASE 3; CASPASE 9; CELL LINE; CELL SURVIVAL; COMET ASSAY; DNA DAMAGE; KIDNEY TUBULES, PROXIMAL; MEMBRANE POTENTIAL, MITOCHONDRIAL; MICRONUCLEUS TESTS; MUTAGENS; RATS; REACTIVE OXYGEN SPECIES; URANIUM;

MAMMALIA; RATTUS;

EID: 34547830253     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfm130     Document Type: Article

References (27)

1. Aten, J. A., Stap, J., Krawczyk, P. M., van Oven, C. H., Hoebe, R. A., Essers, J., and Kanaar, R. (2004). Dynamics of DNA double-strand breaks revealed by clustering of damaged chromosome domains. Science 303, 92-95.
2. E) proximal cell cytotoxicity. Chem. Res. Toxicol. 17, 446-452.
3. Collins, A. R. (2004). The comet assay for DNA damage and repair. Principles, applications and limitations. Mol. Biotechnol. 26 249-261.
4. Fenech, M. (2000). The in vitro micromicleus technique. Mutat. Res. 455, 81-95.
5. Kalinich, J. F., Ramakrishnan, N., Villa, V., and McClain, D. E. (2002). Depleted uranium-uranyl chloride induces apoptosis in mouse J774 macrophages. Toxicology 179, 105-114.
6. Knobel, Y., Glei, M., Weise, A., Osswald, K., Schaferhenrich, A., Richter, K. K., Claussen, U., and Pool-Zobel, B. L. (2006). Uranyl nitrilotriacetate, a stabilized salt of uranium, is genotoxic in nontransformed human colon cells and in the human colon adenoma cell line LT97. Toxicol. Sci. 93, 286-297.
7. Kurttio, P., Harmoinen, A., Saha, H., Salonen, L., Karpas, Z., Komulainen, H., and Auvinen, A. (2006). Kidney toxicity of ingested uranium from drinking water. Am. J. Kidney Dis. 47, 972-982.
8. Leggett, R. W. (1989). The behavior and chemical toxicity of U in the kidney: A reassessment. Health Phys. 57, 365-383.
9. Leggett, R. W., and Pellmar, T. C. (2003). The biokinetics of uranium migrating from embedded DU fragments. J. Environ. Radioact. 64 205-225.
10. Lin, R. H., Wu, L. J., Lee, C. H., and Lin-Shiau, S. Y. (1993). Cytogenetic toxicity of uranyl nitrate in Chinese hamster ovary cells. Mutat. Res. 319, 197-203.
11. Miller, A. C., Brooks, K., Stewart, M., Anderson, B., Shi, L., McClain, D., and Page, N. (2003) Genornic instability in human osteoblast cells after exposure to depleted uranium: Delayed lethality and micronuclei formation. J. Environ. Radioact. 64, 247-259.
12. Miller, A. C., Stewart, M., Brooks, K., Shi, L., and Page, N. (2002). Depleted uranium-catalyzed oxidative DNA damage: Absence of significant alpha particle decay. J. Inorg. Biochem. 91, 246-252.
13. Mirto, H., Barrouillet, M. P., Henge-Napoli, M. H., Ansoborlo, E., Fournier, M., and Cambar, J. (1999). Influence of uranium(VI) speciation for the evaluation of in vitro uranium cytotoxicity on LLC-PKI cells. Hum. Exp. Toxicol. 18, 180-187.
14. Monleau, M., De, Meo, M., Paquet, F., Chazel, V., Dumenil, G., and Donnadieu-Claraz, M. (2006). Genotoxic and inflammatory effects of depleted uranium particles inhaled by rats. Toxicol. Sci. 89 287-295.
15. Mosmann, T. (1983). Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods 65, 55-63.
16. Oh, S.H., Lim, S.C., (2006). A rapid and transient ROS generation by cadmium triggers apoptosis via caspase-dependent pathway in HepG2 cells and this is inhibited through N-acetylcysteine-mediated catalase upregulation. Toxicol Appl Pharmacol. 212, 212-223.
17. Prat, O., Berenguer, F., Malard, V., Tavan, E., Sage, N., Steinmetz, G., and Quemeneur, E. (2005). Transcriptomic and proteomic responses of human renal HEK293 cells to uranium toxicity. Proteomics 5, 297-306.
18. Pulido, M. D., and Parrish, A. R. (2003). Metal-induced apoptosis: Mechanisms. Mutat. Res. 533, 227-241.
19. Singh, N. P., McCoy, M. T., Tice, R. R., and Schneider, E. L. (1988). A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175, 184-191.
20. Stearns, D. M., Yazzie, M., Bradley, A. S., Coryell, V. H., Shelley, J. T., Ashby, A., Asplund, C. S., and Lantz, R. C. (2005). Uranyl acetate induces hprt mutations and uranium-DNA adducts in Chinese hamster ovary EM9 cells. Mutagenesis 20, 417-423.
21. Surralles, J., Antoccia, A., Creus, A., Degrassi, F., Peris, F., Tanzarella, C., Xamena, N., and Marcos, R. (1994). The effect of cytochalasin-B concentration on the frequency of micronuclei induced by four standard mutagens. Results from two laboratories. Mutagenesis 9, 347-353.
22. Taulan, M., Paquet, F., Maubert, C., Delissen, O., Demaille, J., and Romey, M. C. (2004). Renal toxicogenomic response to chronic uranyl nitrate insult in mice. Environ. Health Perspect. 112, 1628-1635.
23. Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J. C., and Sasaki, Y. F. (2000). Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35, 206-221.
24. Twomey, C., and McCarthy, J. V. (2005). Pathways of apoptosis and importance in development. J. Cell. Mol. Med. 9, 345-359.
25. Wan, B., Fleming, J. T., Schultz, T. W., and Sayler, G. S. (2006). In vitro immune toxicity of depleted uranium: Effects on murine macrophages, CD4+ T cells, and gene expression profiles. Environ. Health Perspect. 114, 85-91.
26. Yazzie, M., Gamble, S. L., Civitello, E. R., and Stearns, D. M. (2003). Uranyl acetate causes DNA single strand breaks in vitro in the presence of ascorbate (vitamin C). Chem. Res. Toxicol. 16, 524-530.
27. Zamora, M. L., Tracy, B. L., Zielinski, J. M., Meyerhof, D. P., and Moss, M. A. (1998). Chronic ingestion of uranium in drinking water: A study of kidney bioeffects in humans. Toxicol. Sci. 43, 68-77.