Molecular cloning, expression, purification and crystallographic analysis of PRRSV 3CL protease

Acta Crystallographica Section F: Structural Biology and Crystallization Communications

Volumn 63, Issue 8, 2007, Pages 720-722

  Tian, Xinsheng ^a,b   Feng, Youjun ^a,b   Zhao, Tiezhu ^c   Peng, Hao ^a   Yan, Jinghua ^a   Qi, Jianxun ^b,d   Jiang, Fan ^d   Tian, Kegong ^c   Gao, Feng ^a,e  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c China Animal Disease Control Center   (China)

^d INSTITUTE OF PHYSICS   (China)

^e CHINA AGRICULTURAL UNIVERSITY   (China)

Author keywords

Chemical cross linking; Nsp4 (3C like serine protease, main proteinase); Porcine reproductive and respiratory syndrome; PRRSV

Indexed keywords

PEPTIDE HYDROLASE;

ARTERIVIRUS; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PHYSIOLOGY; X RAY CRYSTALLOGRAPHY;

CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; GENE EXPRESSION REGULATION, ENZYMOLOGIC; PEPTIDE HYDROLASES; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS;

ARTERIVIRUS; ESCHERICHIA COLI; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; RNA VIRUSES; SUS;

EID: 34547631777     PISSN: 17443091     EISSN: 17443091     Source Type: Journal    
DOI: 10.1107/S1744309107033234     Document Type: Article

References (15)

1. Aken, D. van, Benckhuijsen, W. E., Drijfhout, J. W., Wassenaar, A. L., Gorbalenya, A. E. & Snijder, E. J. (2006). Virus Res. 120, 97-106.
2. Anand, K., Palm, G. J., Mesters, J. R., Siddell, S. G., Ziebuhr, J. & Hilgenfeld, R. (2002). EMBO J. 21, 3213-3224.
3. Anand, K., Ziebuhr, J., Wadhwani, P., Mesters, J. R. & Hilgenfeld, R. (2003). Science, 300, 1763-1767.
4. Barrette-Ng, I. H., Ng, K. K., Mark, B. L., Van Aken, D., Cherney, M. M., Garen, C., Kolodenko, Y., Gorbalenya, A. E., Snijder, E. J. & James, M. N. (2002). J. Biol. Chem. 277, 39960-39966.
5. Birtley, J. R., Knox, S. R., Jaulent, A. M., Brick, P., Leatherbarrow, R. J. & Curry, S. (2005). J. Biol. Chem. 280, 11520-11527.
6. Boon, J. A. den, Snijder, E. J., Chirnside, E. D., de Vries, A. A., Horzinek, M. C. & Spaan, W. J. (1991). J. Virol. 65, 2910-2920.
7. Dea, S., Gagnon, C. A., Mardassi, H., Pirzadeh, B. & Rogan, D. (2000). Arch. Virol. 145, 659-688.
8. Ma, G., Feng, Y., Gao, F., Wang, J., Liu, C. & Li, Y. (2005). Biochem. Biophys. Res. Commun. 337, 1301-1307.
9. Matthews, B. W. (1968). J. Mol. Biol. 33, 491-497.
10. Navaza, J. (1994). Acta Cryst. A50, 157-163.
11. Otwinowski, Z. & Minor, W. (1997). Methods Enzymol. 276, 307-326.
12. Paton, D. J., Brown, I. H., Edwards, S. & Wensvoort, G. (1991). Vet. Rec. 128, 617.
13. Snijder, E. J., Wassenaar, A. L., van Dinten, L. C., Spaan, W. J. & Gorbalenya, A. E. (1996). J. Biol. Chem. 271, 4864-4871.
14. Tian, K. et al. (2007). PLoS ONE, 2, e526.
15. Yang, H., Yang, M., Ding, Y., Liu, Y., Lou, Z., Zhou, Z., Sun, L., Mo, L., Ye, S., Pang, H., Gao, G. F., Anand, K., Bartlam, M., Hilgenfeld, R. & Rao, Z. (2003). Proc. Natl Acad. Sci. USA, 100, 13190-13195.