Yellow fever virus NS3 protease: Peptide-inhibition studies

Journal of General Virology

Volumn 88, Issue 8, 2007, Pages 2223-2227

  Löhr, Kristina ^a   Knox, John E ^a   Phong, Wai Yee ^a   Ma, Ngai Ling ^a   Yin, Zheng ^a   Sampath, Aruna ^a   Patel, Sejal J ^a   Wang, Wei Ling ^a   Chan, Wai Ling ^a   Ranga Rao, K R ^a   Wang, Gang ^a   Vasudevan, Subhash G ^a   Keller, Thomas H ^a   Lim, Siew Pheng ^a  

^a NOVARTIS INSTITUTE FOR TROPICAL DISEASES   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

NONSTRUCTURAL PROTEIN 2B; NONSTRUCTURAL PROTEIN 3; PROTEIN INHIBITOR; PROTEINASE; PROTEINASE INHIBITOR; TETRAPEPTIDE; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CATALYSIS; CONTROLLED STUDY; DENGUE VIRUS; DRUG DESIGN; ENZYME ACTIVITY; ENZYME DEGRADATION; ENZYME INHIBITION; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; HYDROLYSIS KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION; VIRUS RECOMBINANT; WEST NILE FLAVIVIRUS; YELLOW FEVER FLAVIVIRUS;

AMINO ACID SEQUENCE; ANTIVIRAL AGENTS; BINDING SITES; ENZYME INHIBITORS; KINETICS; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; RECOMBINANT PROTEINS; RNA HELICASES; SEQUENCE ALIGNMENT; SERINE ENDOPEPTIDASES; SUBSTRATE SPECIFICITY; VIRAL NONSTRUCTURAL PROTEINS; YELLOW FEVER VIRUS;

DENGUE VIRUS; ESCHERICHIA COLI; FLAVIVIRUS; WEST NILE VIRUS; YELLOW FEVER VIRUS;

EID: 34547578902     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/vir.0.82735-0     Document Type: Article

References (27)

1. Amberg, S. M., Nestorowicz, A., McCourt, D. W. & Rice, C. M. (1994). NS2B-3 proteinase-mediated processing in the yellow fever virus structural region: in vitro and in vivo studies. J Virol 68, 3794-3802.
2. Barrett, A. D. & Higgs, S. (2007). Yellow fever: a disease that has yet to be conquered. Annu Rev Entomol 52, 209-229.
3. Bazan, J. F. & Retterick. R. J. (1989). Detection of a trypsin-like serine protease domain in flaviviruses and pestiviruses. Virology 171, 637-639.
4. Bessaud, M., Pastorino, B. A. M., Peyrefitte, C. N., Rolland, D., Grandadam, M. & Tolou, H. J. (2006). Functional characterization of the N2B/NS3 protease complex from seven viruses belonging to different groups inside the genus Flavivirus. Virus Res 120, 79-90.
5. Chambers, T. J., Hahn, C. S., Galler, R. & Rice, C. M. (1990a). Flavivirus genome organization, expression, and replication. Annu Rev Microbiol 44, 649-688.
6. Chambers, T. J., Weir, R. C., Grakoui, A., McCourt, D. W., Bazan, J. F., Fletterick, R. J. & Rice, C. M. (1990b). Evidence that the N-terminal domain of nonstructural protein NS3 from yellow fever virus is a serine protease responsible for site-specific cleavages in the viral polyprotein. Proc Natl Acad Sci U S A 87, 8898-8902.
7. Chambers, T. J,, Grakoul, A. & Rice, C. M. (1991). Processing of the yellow fever virus nonstructural polyprotein: a catalytically active NS3 proteinase domain and NS2B are required for cleavages at dibasic sites. J Virol 65, 6042-6050.
8. Chambers, T. J., Nestorowicz, A., Amberg, S. M. & Rice, C. M. (1993). Mutagenesis of the yellow fever virus NS2B protein: effects on proteolytic processing, NS2B-NS3 complex formation, and viral replication. J Virol 67, 6797-6807.
9. Chambers, T. J., Nestorowicz, A. & Rice, C. M. (1995). Mutagenesis of the yellow fever virus NS2B/3 cleavage site: determinants of cleavage site specificity and effects on polyprotein processing and viral replication. J Virol 69, 1600-1605.
10. Chambers, T. J., Droll, A. D., Tang, Y., Liang, Y., Ganesh, V. K., Murthy, K. H. M. & Nickells, M. (2005). Yellow fever virus NS2B-NS3 protease: characterization of charged-to-alanine mutant and revertant viruses and analysis of polyprotein-cleavage activities. J Gen Virol 86, 1403-1413.
11. Chappell, K. J., Stoermer, M. J., Fairlie, D. P. & Young, P. R. (2006). West Nile virus NS3 protease: insights into substrate binding and processing through combined modelling, protease mutagenesis and kinetic studies. J Biol Chem 281, 38448-38458.
12. Copeland, R. A. (2000). Enzymes: a Practical Introduction to Structure, Mechanism and Data Analysis, 2nd edn. New York: Wiley.
13. Droll, D. A., Murthy, H. M. K. & Chambers, T. J. (2000). Yellow fever virus NS2B-NS3 protease: charged-to-alanine mutagenesis and deletion analysis define regions important for protease complex formation and function. Virology 275, 335-347.
14. Erbel, P., Schiering, N., D'Arcy, A., Renatus, M., Kroemer, M., Lim, S. P., Yin, Z., Keller, T. H., Vasudevan, S. G. & Hommel, U. (2006). Structural basis for the activation of flaviviral NS3 proteases from dengue and West Nile virus. Nat Struct Mol Biol 13, 372-373.
15. Falgout, B., Pethel, M., Zhang, Y. M. & Lai, C. J. (1991). Both nonstructural proteins NS2B and NS3 are required for the proteolytic processing of dengue virus nonstructural proteins. J Virol 65, 2467-2475.
16. Falgout, B., Miller, R. H. & Lai, C. J. (1993). Deletion analysis of dengue virus type 4 nonstructural protein NS2B: identification of a domain required for NS2B-NS3 protease activity. J Virol 67, 2034-2042.
17. Ganesh, V. K., Muller, N., Judge, K., Luan, C. H., Padmanabhan, R. & Murthy, K. H. (2005). Identification and characterization of nonsubstrate based inhibitors of the essential dengue and West Nile virus proteases. Bioorg Med Chem 13, 257-264.
18. Gorbalenya, A. E., Donchenko, A. P., Koonin, E. V. & Blinov, V. M. (1989). N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases. Nucleic Acids Res 17, 3889-3897.
19. Knox, J. E., Ma, N. L., Yin, Z., Patel, S. J., Wang, W. L., Chan, W. L., Range Rao, K. R., Wang, G., Ngew, X. & other authors (2006). Peptide inhibitors of West Nile NS3 protease: SAR study of tetrapeptide aldehyde inhibitors. J Med Chem 49, 6585-6590.
20. Leung, D., Schroder, K., White, H., Fang, N. X., Stoermer, M. J., Abbenante, G., Martin, J. L., Young, P. R. & Fairlie, D. P. (2001). Activity of recombinant dengue 2 virus NS3 protease in the presence of a truncated NS2B co-factor, small peptide substrates, and inhibitors. J Biol Chem 276, 45762-45771.
21. Li, J., Lim, S. P., Beer, D., Patel, V., Wen, D., Tumanut, C., Tully, D. C., Williams, J. A., Jiricek, J. & other authors (2005). Functional profiling of recombinant NS3 proteases from all four serotypes of dengue virus using tetra- and octa-peptide substrate libraries. J Biol Chem 280, 28766-28774.
22. Nall, T. A., Chappell, K. J., Stoermer, M. J., Fang, N. X., Tyndall, J. D., Young, P. R. & Fairlie, D. P. (2004). Enzymatic characterization and homology model of a catalytically active recombinant West Nile virus NS3 protease. J Biol Chem 279, 48535-48542.
23. Niyomrattanakit, P., Yahorava, S., Mutule, I., Mutulis, F., Petrovska, R., Prusis, P., Katzenmeier, G. & Wikberg, J. E. (2006). Probing the substirate specificity of the dengue virus type 2 NS3 serine protease by using internally quenched fluorescent peptides. Biochem J 397, 203-211.
24. Rice, C. M., Lenches, E. M., Eddy, S. R., Shin, S. J., Sheets, R. L. & Strauss, J. H. (1985). Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229, 726-733.
25. Yin, Z., Patel, S. J., Wang, W.-L., Wang, G., Chan, W. L., Ranga Rao, K. R., Alam, J., Jeyaraj, D. A., Ngew, X. & other authors (2006a). Peptide inhibitors of dengue virus NS3 protease. 1. Warhead. Bioorg Med Chem Lett 16, 36-39.
26. Yin, Z., Patel, S. J., Wang, W.-L., Chan, W. L., Ranga Rao, K. R., Wang, G., Ngew, X., Patel, V., Beer, D. & other authors (2006b). Peptide inhibitors of dengue virus NS3 protease. 2. SAR study of tetrapeptide aldehyde inhibitors. Bioorg Med Chem Lett 16, 40-43.
27. Yusof, R., Clum, S., Wetzel, M., Murthy, H. M. & Padmanabhan, R. (2000). Purified NS2B/NS3 serine protease of dengue virus type 2 exhibits cofactor NS2B dependence for cleavage of substrates with dibasic amino acids in vitro. J Biol Chem 275, 9963-9969.