Identification and characterization of 3-substituted pyrazolyl esters as alternate substrates for cathepsin B: The confounding effects of DTT and cysteine in biological assays

Bioorganic and Medicinal Chemistry Letters

Volumn 17, Issue 17, 2007, Pages 4761-4766

  Myers, Michael C ^a   Napper, Andrew D ^a   Motlekar, Nuzhat ^a   Shah, Parag P ^a   Chiu, Chun Hao ^a   Beavers, Mary Pat ^a   Diamond, Scott L ^a   Huryn, Donna M ^a   Smith III, Amos B ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Alternate substrate; Cathepsin B; Cysteine; DTT; HTS; MLSMR; Pyrazole esters

Indexed keywords

CATHEPSIN B; CYSTEINE; DITHIOTHREITOL; ESTER DERIVATIVE; PYRAZOLE; PYRAZOLYL ESTER; UNCLASSIFIED DRUG;

ARTICLE; BIOASSAY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME STRUCTURE; HIGH THROUGHPUT SCREENING;

BIOLOGICAL ASSAY; CARBON; CATHEPSIN B; CHEMISTRY, PHARMACEUTICAL; CYSTEINE; DITHIOTHREITOL; DOSE-RESPONSE RELATIONSHIP, DRUG; DRUG DESIGN; ESTERS; HUMANS; INHIBITORY CONCENTRATION 50; MODELS, CHEMICAL; PROTEIN BINDING; REDUCING AGENTS; TIME FACTORS;

EID: 34547506020     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2007.06.091     Document Type: Article

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