pHYPER, a shRNA vector for high-efficiency RNA interference in embryonic stem cells

BioTechniques

Volumn 42, Issue 6, 2007, Pages 738-743

  Berlivet, Soizik ^a   Guiraud, Virginie ^a   Houlard, Martin ^a   Gérard, Matthieu ^a,b  

^a SERVICE DE CHIMIE BIOORGANIQUE ET DE MARQUAGE   (France)

^b CEA SACLAY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CELLS; ENZYMES; GENE EXPRESSION; OPTIMIZATION; SYNTHESIS (CHEMICAL);

EPIFLUORESCENCE; PLASMID VECTORS; TRANSIENT TRANSFECTION;

RNA;

DNA DIRECTED RNA POLYMERASE III; GREEN FLUORESCENT PROTEIN; PLASMID VECTOR; SHORT HAIRPIN RNA;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; EMBRYO; EMBRYONIC STEM CELL; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENE FUNCTION; GENETIC TRANSFECTION; GENOME; MOUSE; NONHUMAN; PLASMID; PROMOTER REGION; RNA INTERFERENCE; RNA SYNTHESIS;

ANIMALS; CELL LINE; CROSSES, GENETIC; EMBRYONIC STEM CELLS; GENETIC VECTORS; MICE; MICE, INBRED C3H; MICE, TRANSGENIC; RNA INTERFERENCE; RNA, SMALL INTERFERING;

MAMMALIA;

EID: 34447128161     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000112454     Document Type: Article

References (19)

1. Elbashir, S.M., J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl. 2001. Duplexes of 21-nucleotide RNAs mediate RVA interference in cultured mammalian cells. Nature 411:494-498.
2. Brummelkamp, T.R., R. Bernards, and R. Agami. 2002. A system for stable expression of short interfering RNAs in mammalian cells. Science 296:550-553.
3. Paddison, P.J., A.A. Caudy, E. Bernstein, G.J. Hannon, and D.S. Conklin. 2002. Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells. Genes Dev. 16:948-958.
4. Paul, C.P., P.D. Good, I. Winer, and D.R. Engelke. 2002. Effective expression of small interfering RNA in human cells. Nat. Biotechnol. 20:505-508.
5. Sui, G., C. Soohoo, B. Affar el, F. Gay, Y. Shi, and W.C. Forrester. 2002. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci USA. 99:5515-5520.
6. Miyagishi, M. and K. Taira. 2002. U6 promoter-driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells. Nat. Biotechnol. 20:497-500.
7. Lee, N.S., T. Dohjima, G. Bauer, H. Li, M.J. Li, A. Ehsani, P. Salvaterra, and J. Rossi. 2002. Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells. Nat. Biotechnol. 20:500-505.
8. Valencia-Sanchez, M.A., J. Liu, G.J. Hannon, and R. Parker. 2006. Control of translation and mRNA degradation by miRNAs and siRNAs. Genes Dev. 20:515-524.
9. Schwarz, D.S., G. Hutvagner, T. Du, Z. Xu, N. Aronin, and P.D. Zamore. 2003. Asymmetry in the assembly of the RNAi enzyme complex. Cell 115:199-208.
10. Khvorova, A., A. Reynolds, and S.D. Jayasena. 2003. Functional siRNAs and miRNAs exhibit strand bias. Cell 115:209-216.
11. Silva, J.M., M.Z. Li, K. Chang, W. Ge, M.C. Golding, R.J. Rickles, D. Siolas, G. Hu, et al. 2005. Second-generation shRNA libraries covering the mouse and human genomes. Nat. Genet. 37:1281-1288.
12. Kunath, T., G. Gish, H. Lickert, N. Jones, T. Pawson, and J. Rossant. 2003. Transgenic RNA interference in ES cell-derived embryos recapitulates a genetic null phenotype. Nat. Biotechnol. 21:559-561.
13. Fritsch, L., L.A. Martinez, R. Sekhri, I. Naguibneva, M. Gerard, M. Vandromme, L. Schaeffer, and A. Harel-Bellan. 2004. Conditional gene knock-down by CRE-dependent short interfering RNAs. EMBO Rep. 5:178-182.
14. Houlard, M., S. Berlivet, A.V. Probst, J.P. Quivy, P. Hery, G. Almouzni, and M. Gerard. 2006. CAF-1 is essential for heterochromatin organization in pluripotent embryonic cells. PLoS Genet 2:e181.
15. Abbondanzo, S.J., I. Gadi, and C.L. Stewart. 1993. Derivation of embryonic stem cell lines. Methods Enzymol. 225:803-823.
16. Niwa, H., K. Yamamura, and J. Miyazaki. 1991. Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-199.
17. Tessarollo, L. 2001. Manipulating mouse embryonic stem cells. Methods Mol. Biol. 158:47-63.
18. Gerard, M., D. Duboule, and J. Zakany. 1993. Structure and activity of regulatory elements involved in the activation of the Hoxd-11 gene during late gastrulation. EMBO J. 12:3539-3550.
19. Myslinski, E., J.C. Ame, A. Krol, and P. Carbon. 2001. An unusually compact external promoter for RNA polymerase III transcription of the human H1RNA gene. Nucleic Acids Res. 29:2502-2509.