Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS

FEBS Journal

Volumn 272, Issue 13, 2005, Pages 3376-3386

  Suginta, Wipa ^a   Vongsuwan, Archara ^a   Songsiriritthigul, Chomphunuch ^a,b   Svasti, Jisnuson ^c   Prinz, Heino ^d  

^a SURANAREE UNIVERSITY OF TECHNOLOGY   (Thailand)

^b National Synchrotron Research Center Thailand   (Thailand)

^c MAHIDOL UNIVERSITY   (Thailand)

^d MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

Author keywords

Chitinase A; Chitooligosaccharides; Quantitative HPLC MS; Transglycosylation; Vibrio carchariae

Indexed keywords

CHITIN DERIVATIVE; CHITINASE; CHITINASE A; CHITOSE OLIGOSACCHARIDE; DNA; OLIGOSACCHARIDE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; ELECTROSPRAY MASS SPECTROMETRY; ENZYME ACTIVE SITE; ENZYME ANALYSIS; ENZYME GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; KINETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; POINT MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SITE DIRECTED MUTAGENESIS; STEADY STATE; STEREOCHEMISTRY; SYNTHESIS; VIBRIO; VIBRIO HARVEYI;

BINDING SITES; CATALYSIS; CHITIN; CHITINASE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLYCOSYLATION; HYDROLYSIS; MASS SPECTROMETRY; MUTAGENESIS, SITE-DIRECTED; OLIGOSACCHARIDES; POINT MUTATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; VIBRIO;

VIBRIO HARVEYI;

EID: 22144480668     PISSN: 1742464X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2005.04753.x     Document Type: Article

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