Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot

Nucleic Acids Research

Volumn 35, Issue 8, 2007, Pages

  Pall, Gurman Singh ^a   Codony Servat, Carles ^a   Byrne, Jane ^a   Ritchie, Leigh ^a   Hamilton, Andrew ^a,b  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

^b Stirling Royal Infirmary   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CYANAMIDE; MICRORNA; PHOSPHATE; RNA; SMALL INTERFERING RNA; 1 ETHYL 3 (3 (DIETHYLAMINO)PROPYL)CARBODIIMIDE; 1-ETHYL-3-(3-(DIETHYLAMINO)PROPYL)CARBODIIMIDE; CROSS LINKING REAGENT; NYLON; PLANT RNA; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; CELL CULTURE; CELL LINE; CONTROLLED STUDY; CROSS LINKING; ELECTROPHORESIS; FEMALE; GENE EXPRESSION; HELA CELL; HUMAN; HUMAN CELL; MALE; MAMMAL CELL; MOUSE; NONHUMAN; NORTHERN BLOTTING; PLANT GROWTH; PRIORITY JOURNAL; RNA ANALYSIS; RNA EXTRACTION; RNA HYBRIDIZATION; SENSITIVITY ANALYSIS; ULTRAVIOLET RADIATION; VALIDATION PROCESS; ANIMAL; CHEMISTRY; EVALUATION; METHODOLOGY; OLIGONUCLEOTIDE PROBE; RAT; TEMPERATURE;

MAMMALIA;

ANIMALS; BLOTTING, NORTHERN; CARBODIIMIDES; CELL LINE; CROSS-LINKING REAGENTS; HUMANS; MALE; MICE; MICRORNAS; NYLONS; OLIGONUCLEOTIDE PROBES; RATS; RNA, PLANT; RNA, SMALL INTERFERING; TEMPERATURE; ULTRAVIOLET RAYS;

EID: 34250374820     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkm112     Document Type: Article

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