Characteristic gene expression induced by polyurethane foam/spheroid culture of hepatoma cell line, Hep G2 as a promising cell source for bioartificial liver

Hepato-Gastroenterology

Volumn 54, Issue 75, 2007, Pages 814-820

  Shimada, Mitsuo ^a   Yamashita, Yo Ichi ^b   Tanaka, Shinji ^b   Shirabe, Ken ^b   Nakazawa, Kohji ^c   Ijima, Hiroyuki ^c   Sakiyama, Ryoichi ^c   Fukuda, Junji ^c   Funatsu, Kazumori ^c   Sugimachi, Keizo ^b  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c KYUSHU UNIVERSITY   (Japan)

Author keywords

Albumin; Ammonia; cDNA microarray; Differentiation

Indexed keywords

ALBUMIN; AMMONIA; ANGIOTENSIN; COMPLEMENTARY DNA; GLUCOCORTICOID; GLUTATHIONE TRANSFERASE; GROWTH FACTOR; POLYURETHAN FOAM;

ARTICLE; BIOARTIFICIAL LIVER; CANCER CELL CULTURE; CELL FUNCTION; CELL STRAIN HEPG2; CELL STRUCTURE; CONTROLLED STUDY; CULTURE MEDIUM; DNA MICROARRAY; DOWN REGULATION; GENE CONTROL; GENE EXPRESSION; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER CELL; LIVER FUNCTION TEST; MICROARRAY ANALYSIS; MONOLAYER CULTURE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SPHEROID CELL; STRESS; UPREGULATION;

AMMONIA; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; GENE EXPRESSION; GENE EXPRESSION PROFILING; HEPATOCYTES; HUMANS; LIVER NEOPLASMS; LIVER, ARTIFICIAL; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYURETHANES; SPHEROIDS, CELLULAR; TUMOR CELLS, CULTURED;

EID: 34250198579     PISSN: 01726390     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Gerlach JC, Ziemer R, Neuhaus P: Fulminant liver failure: relevance of extracorporeal hybrid liver support systems. Int J Artif Organs 1996; 19:7-13.
2. Rozga J, Demetriou AA: Artificial liver: evolution and perspectives. ASAIO J 1995; 41:831-837.
3. Watanabe FD, Mullon CJ, Hewitt WR, Arkadopoulos N, Kahaku E, Eguchi S, Khalili T, Arnaut W, Shackleton CR, Rozga J, Solomon B, Demetriou AA: Clinical experience with a bioartificial liver in the treatment of severe liver failure: a phase I clinical study. Ann Surg 1997; 225:484-494.
4. Gerlach, JC: Development of a hybrid liver support system: a review. Int J Artif Organs 1996; 19:645-654.
5. Sussman NL, Chong MG, Koussayer T, He DE, Shang TA, Whisennand HH, Kelly JH: Reversal of fulminant hepatic failure using an extracorporeal liver assist device. Hepatology 1992; 16:60-65.
6. Koide N, Sakaguchi K, Koide Y, et al: Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other non-adherent environments. Exp Cell Res 1990; 186:227-235.
7. Kawaguchi M, Koide N, Sakaguchi K, Asano F, Mori M, Tsuji T: Bilirubin conjugation by multicellular spheroid of adult rat hepatocytes in primary culture. Jpn J Artif Organs 1989; 18:269-272.
8. Matsushita T, Ketayama M, Kamihata K, Funatsu K: Anchorage-dependent mammalian cell culture using polyurethane foam as a new sbstratum for cell attachment. Appl Microbiol Biotechnol 1990; 33:287-290.
9. Matsushita T, Ijima H, Koide N, Funatsu K: High albumin production by multicellular spheroid of adult rat hepatocytes formed in the pores of polyurethane foam. Appl Microbiol Biotechnol 1991; 36:324-326.
10. Nakazawa K, Matsushita T, Funatsu K: Prolonged lidocaine metabolizing activity of primary hepatocytes with spheroid culture using polyurethane foam as a culture sbstratum. Cytotechnology 1997; 24:235-242.
11. Nakazawa K, Mizumoto H, Kaneko M, Ijima H, Gion T, Shimada M, Shirabe K, Takenaka K, Sugimachi K, Funatsu K: Formation of porcine hepatocyte spherical multicellular aggregates (spheroid) and analysis of drug metabolic functions. Cytotechnology 1999; 31:61-68.
12. Ijima H, Nakazawa K, Mizumoto H, Matsushita T, Funatsu K: Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane formed as a cell culture substratum and its application to a hybrid artificial liver. J Biomat Sci Polymer Edn 1998; 9:765-778.
13. Ijima H, Matsushita T, Nakazawa K, Fujii Y, Funatsu K: Hepatocyte spheroids in polyurethane forms: functional analysis and application for a hybrid artificial liver. Tissue Eng 1998; 4:213.
14. Gion T, Shimada M, Shirabe K, Nakazawa K, Ijima H, Matsushita T, Funatsu K, Sugimachi K: Evaluation of a hybrid artificial liver using a polyurethane foam packed-bed culture system in dogs. J Surg Res 1999; 82:131-136.
15. Kaneko M, Fukuda J, Ijima H, Nakazawa K, Gion T, Shimada M, Shirabe K, Takenaka K, Sugimachi K, Funatsu K: Development of hybrid artificial liver support system using spheroid culture and application to warm ischemic liver failure in dog and pig as a preclinical test. Materials Sci Engineer 1998; 6:245-248.
16. Patience C, Takeuchi Y, Weiss RA: Infection of human cells by an endogenous retrovirus of pigs. Nature Med 1999; 5:282-286.
17. van der Laan LJ, Lockey C, Griffeth BC, Frasier FS, Wilson CA, Onions DE, Hering BJ, Long Z, Otto E, Torbett BE, Salomon DR: Infection by porcine endogenous retrovirus after islet xenotransplantation in SCID mice. Nature 2000; 407:90-94.
18. Ellis AJ, Hughes RD, Wendon JA, Dunne J, Langley PG, Kelly JH, Giaslason GT, Sussman NL, Williams R: Pilot-controlled trial of the extracorporeal liver assist device in acute liver failure. Hepatology 1996; 24:1446-1451.
19. Yamashita Y, Shimada M, Tsujita E, Tanaka S, Ijima H, Nakazawa K, Sakiyama R, Fukuda J, Ueda T, Funatsu K, Sugimachi K: Polyurethane foam/spheroid culture system using human hepatoblastoma cell line (Hep G2) as a possible new hybrid artificial liver. Cell Transplant 2001; 10:717-722.
20. Alwine JC, Kemp DJ, Stark GR: Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA 1977; 74:5350-5354.
21. Liang P, Pardee AB: Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 1992; 257:967-997.
22. Velculescu VE, Zhang L, Vogelstein B, Kinzier KW: Serial analysis of gene expression. Science 1995; 270:484-487.
23. Lennon GG, Lehrach H: Hybridization analyses of arrayed cDNA libraries. Trend Genet 1991; 7:314-317.
24. Duggan DJ, Bittner M, Chen Y, Meltzer P, Trent JM: Expression profiling using cDNA microarrays. Nat Genet 1999; 21:10-14.
25. Schena M, Shalon D, Davis RW, Brown PO: Quantitative monitoring of gene expression patterns with a complementary DNA microarray (Comments). Science 1995; 270:467-470.
26. DeRisi J, Penland L, Brown PO, Bittner ML, Meltzer PS, Ray M, Chen Y, et al: Use of a cDNA microarray to analyse gene expression patterns in human cancer. Nat Genet 1996; 14:457-460.
27. Brown PO, Botstein D: Exploring the new world of the genome with DNA microarrays. Nat Genet 1999; 21:33-37.
28. Kawai H, Kaneko S, Honda M, Shirota Y, Kobayashi K: alpha-fetoprotein-producing hepatoma cell lines share common expression profiles of genes in various categories demonstrated by cDNA microarray analysis. Hepatology 2001; 33:676-691.
29. Khaoustov VI, Risin D, Pellis NR, Yoffe B: Microarray analysis of genes differentially expressed in Hep G2 cells cultured in simulated microgravity: preliminary report. In Vitro Cell Dev Biol 2001; 37:84-88.
30. Yamashita Y, Shimada M, Tsujita E, Shirabe K, Ijima H, Nakazawa K, Sakiyama R, Fukuda J, Funatsu K, Sugimachi K: High metabolic function of primary human and porcine hepatocytes in a polyurethane foam/spheroid culture system in plasma from patients with fulminant hepatic failure. Cell Transplant 2002; 11:379-384.
31. van Wezel AL: Growth of cell-strains and primary cells on micro-carriers in homogeneous culture. Nature 1967; 216:64-65.
32. Wu FJ, Friend JR, Remmel RP, Cerra FB, Hu WS: Enhanced cytochrome P450 IA1 activity of self-assembled rat hepatocyte spheroids. Cell Transplant 1999; 8(3):233-246.
33. Hamamoto R, Yamada K, Kamihira M, Iijima S: Differentiation and proliferation of primary rat hepatocytes cultured as spheroids. J Biochem 1998; 124(5):972-979.
34. Hou DX, Arimura M, Fukuda M, Oka T, Fujii M: Expression of cell adhesion molecule and albumin genes in primary culture of rat hepatocytes. Cell Biol Int 2001; 25(3):239-244.
35. Khalil M, Shariat-Panahi A, Tootle R, Ryder T, McCloskey P, Roberts E, Hodgson H, Selden C: Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function. J Hepatol 2001; 34(1):68-77.
36. Yamashita Y, Shimada M, Harimoto N, Rikimaru T, Shirabe K, Tanaka S, Sugimachi K: Histone deacetylase inhibitor trichostatin a induces cell-cycle arrest/apoptosis and hepatocyte differentiation in human hepatoma cells. Int J Cancer 2003; 103:572-576.