Triptolide suppresses interleukin-1β-induced human β-defensin-2 mRNA expression through inhibition of transcriptional activation of NF-κB in A549 cells

International Journal of Molecular Medicine

Volumn 19, Issue 5, 2007, Pages 757-763

  Jang, Byeong Churl ^a   Lim, Ki Jo ^a   Choi, In Hak ^b   Suh, Min Ho ^a   Park, Jong Gu ^a   Mun, Kyo Chul ^a   Bae, Jae Hoon ^a   Shin, Dong Hoon ^a   Suh, Seong Il ^a  

^a Keimyung University   (South Korea)

^b Inje University   (South Korea)

Author keywords

A549 cells; Human defensin 2; Il 1 ; MKP 1; NF B; Triptolide

Indexed keywords

BETA DEFENSIN; CELL CYCLE PROTEIN; DITERPENE; DUAL SPECIFICITY PHOSPHATASE 1; EPOXIDE; IMMEDIATE EARLY PROTEIN; IMMUNOSUPPRESSIVE AGENT; INTERLEUKIN 1BETA; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE P38; PHENANTHRENE DERIVATIVE; PHOSPHATIDYLINOSITOL 3 KINASE; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN TYROSINE PHOSPHATASE; STRESS ACTIVATED PROTEIN KINASE; TRANSCRIPTION FACTOR RELA; TRIPTOLIDE; UNCLASSIFIED DRUG;

ARTICLE; DOWN REGULATION; DRUG EFFECT; ENZYME ACTIVATION; GENETIC TRANSCRIPTION; GENETICS; HUMAN; METABOLISM; TRANSACTIVATION; TUMOR CELL LINE;

1-PHOSPHATIDYLINOSITOL 3-KINASE; BETA-DEFENSINS; CELL CYCLE PROTEINS; CELL LINE, TUMOR; DITERPENES; DOWN-REGULATION; ENZYME ACTIVATION; EPOXY COMPOUNDS; HUMANS; IMMEDIATE-EARLY PROTEINS; IMMUNOSUPPRESSIVE AGENTS; INTERLEUKIN-1BETA; JNK MITOGEN-ACTIVATED PROTEIN KINASES; P38 MITOGEN-ACTIVATED PROTEIN KINASES; PHENANTHRENES; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN-TYROSINE-PHOSPHATASE; RNA, MESSENGER; TRANS-ACTIVATION (GENETICS); TRANSCRIPTION FACTOR RELA; TRANSCRIPTION, GENETIC;

EID: 34248544967     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.19.5.757     Document Type: Article

References (36)

1. Huttner KM and Bevins CL: Antimicrobial peptides as mediators of epithelial host defense. Pediatr Res 45: 785-794, 1999.
2. Yang D, Chertov O, Bykovskaia SN, Chen Q, Buffo MJ, Shogan J, Anderson M, Schroder JM, Wang JM, Howard OM and Oppenheim JJ: Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science 286: 525-528, 1999.
3. Territo MC, Ganz T, Selsted ME and Lehrer R: Monocyte-chemotactic activity of defensins from human neutrophils. J Clin Invest 84: 2017-2020, 1989. (Pubitemid 20016213)
4. Kaiser V and Diamond G: Expression of mammalian defensin genes. J Leukoc Biol 68: 779-784, 2000.
5. Garcia JR, Krause A, Schulz S, Rodriguez-Jimenez FJ, Kluver E, Adermann K, Forssmann U, Frimpong-Boateng A, Bals R and Forssmann WG: Human beta-defensin 4: a novel inducible peptide with a specific salt-sensitive spectrum of antimicrobial activity. FASEB J 15: 1819-1821, 2001.
6. Yamaguchi Y, Nagase T, Makita R, Fukuhara S, Tomita T, Tominaga T, Kurihara H and Ouchi Y: Identification of multiple novel epididymis-specific beta-defensin isoforms in humans and mice. J Immunol 169: 2516-2523, 2002.
7. Duits LA, Ravensbergen B, Rademaker M, Hiemstra PS and Nibbering PH: Expression of beta-defensin 1 and 2 mRNA by human monocytes, macrophages and dendritic cells. Immunology 106: 517-525, 2002.
8. Harder J, Bartels J, Christophers E and Schroder JM: Isolation and characterization of human beta -defensin-3, a novel human inducible peptide antibiotic. J Biol Chem 276: 5707-5713, 2001.
9. Hao HN, Zhao J, Lotoczky G, Grever WE and Lyman WD: Induction of human beta-defensin-2 expression in human astrocytes by lipopolysaccharide and cytokines. J Neurochem 77: 1027-1035, 2001.
10. Singh PK, Jia HP, Wiles K, Hesselberth J, Liu L, Conway BA, Greenberg EP, Valore EV, Welsh MJ, Ganz T, Tack BF and McCray PB Jr: Production of beta-defensins by human airway epithelia. Proc Natl Acad Sci USA 95: 14961-14966, 1998.
11. Bals R, Wang X, Wu Z, Freeman T, Bafna V, Zasloff M and Wilson JM: Human beta-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J Clin Invest 102: 874-880, 1998. (Pubitemid 28419232)
12. Hiratsuka T, Nakazato M, Date Y, Ashitani J, Minematsu T, Chino N and Matsukura S: Identification of human beta-defensin- 2 in respiratory tract and plasma and its increase in bacterial pneumonia. Biochem Biophys Res Commun 249: 943-947, 1998.
13. Ohara T, Morishita T, Suzuki H, Masaoka T, Nagata H and Hibi T: Pathophysiological role of human beta-defensins 2 in gastric mucosa. Int J Mol Med 14: 1023-1027, 2004.
14. Jang BC, Lim KJ, Paik JH, Kwon YK, Shin SW, Kim SC, Jung TY, Kwon TK, Cho JW, Baek WK, Kim SP, Suh MH and Suh SI: Up-regulation of human beta-defensin 2 by interleukin- 1beta in A549 cells: involvement of PI3K, PKC, p38 MAPK, JNK, and NF-kappaB. Biochem Biophys Res Commun 320: 1026-1033, 2004.
15. Tsutsumi-Ishii Y and Nagaoka I: NF-kappa B-mediated transcriptional regulation of human beta-defensin-2 gene following lipopolysaccharide stimulation. J Leukoc Biol 71: 154-162, 2002.
16. Moon SK, Lee HY, Li JD, Nagura M, Kang SH, Chun YM, Linthicum FH, Ganz T, Andalibi A and Lim DJ: Activation of a Src-dependent Raf-MEK1/2-ERK signaling pathway is required for IL-1alpha-induced upregulation of beta-defensin 2 in human middle ear epithelial cells. Biochim Biophys Acta 1590: 41-51, 2002. (Pubitemid 34626767)
17. Krisanaprakornkit S, Kimball JR and Dale BA: Regulation of human beta-defensin-2 in gingival epithelial cells: the involvement of mitogen-activated protein kinase pathways, but not the NFkappaB transcription factor family. J Immunol 168: 316-324, 2002.
18. McDermott AM, Redfern RL, Zhang B, Pei Y, Huang L and Proske RJ: Defensin expression by the cornea: multiple signalling pathways mediate IL-1beta stimulation of hBD-2 expression by human corneal epithelial cells. Invest Ophthalmol Vis Sci 44: 1859-1865, 2003.
19. Chen BJ: Triptolide, a novel immunosuppressive and antiinflammatory agent purified from a Chinese herb Tripterygium wilfordii Hook F. Leuk Lymphoma 42: 253-265, 2001.
20. Qiu D and Kao PN: Immunosuppressive and anti-inflammatory mechanisms of triptolide, the principal active diterpenoid from the Chinese medicinal herb Tripterygium wilfordii Hook. f. Drugs Res Dev 4: 1-18, 2003.
21. Qiu D, Zhao G, Aoki Y, Shi L, Uyei A, Nazarian S, Ng JC and Kao PN: Immunosuppressant PG490 (triptolide) inhibits T-cell interleukin-2 expression at the level of purine-box/nuclear factor of activated T-cells and NF-kappaB transcriptional activation. J Biol Chem 274: 13443-13450, 1999.
22. Zhao G, Vaszar LT, Qiu D, Shi L and Kao PN: Anti-inflammatory effects of triptolide in human bronchial epithelial cells. Am J Physiol Lung Cell Mol Physiol 279: L958-L966, 2000.
23. Wu Y, Cui J, Bao X, Chan S, Young DO, Liu D and Shen P: Triptolide attenuates oxidative stress, NF-kappaB activation and multiple cytokine gene expression in murine peritoneal macrophage. Int J Mol Med 17: 141-150, 2006.
24. Lin N, Sato T and Ito A: Triptolide, a novel diterpenoid triepoxide from Tripterygium wilfordii Hook. f., suppresses the production and gene expression of pro-matrix metalloproteinases 1 and 3 and augments those of tissue inhibitors of metalloproteinases 1 and 2 in human synovial fibroblasts. Arthritis Rheum 44: 2193-2200, 2001.
25. Zhou HF, Niu DB, Xue B, Li FQ, Liu XY, He QH, Wang XH and Wang XM: Triptolide inhibits TNF-alpha, IL-1 beta and NO production in primary microglial cultures. Neuroreport 14: 1091-1095, 2003.
26. Chen P, Li J, Barnes J, Kokkonen GC, Lee JC and Liu Y: Restraint of proinflammatory cytokine biosynthesis by mitogen-activated protein kinase phosphatase-1 in lipopolysaccharide-stimulated macrophages. J Immunol 169: 6408-6416, 2002.
27. Zhao Q, Shepherd EG, Manson ME, Nelin LD, Sorokin A and Liu Y: The role of mitogen-activated protein kinase phosphatase-1 in the response of alveolar macrophages to lipopolysaccharide: attenuation of proinflammatory cytokine biosynthesis via feedback control of p38. J Biol Chem 280: 8101-8108, 2005.
28. Shepherd EG, Zhao Q, Welty SE, Hansen TN, Smith CV and Liu Y: The function of mitogen-activated protein kinase phosphatase-1 in peptidoglycan-stimulated macrophages. J Biol Chem 279: 54023-54031, 2004.
29. Keyse SM: Protein phosphatases and the regulation of mitogen-activated protein kinase signalling. Curr Opin Cell Biol 12: 186-192, 2000.
30. Li J, Gorospe M, Hutter D, Barnes J, Keyse SM and Liu Y: Transcriptional induction of MKP-1 in response to stress is associated with histone H3 phosphorylation-acetylation. Mol Cell Biol 21: 8213-8224, 2001.
31. Matsushime H, Quelle DE, Shurtleff SA, Shibuya M, Sherr CJ and Kato JY: D-type cyclin-dependent kinase activity in mammalian cells. Mol Cell Biol 14: 2066-2076, 1994.
32. Slomiany BL and Slomiany A: Role of ERK and p38 mitogen-activated protein kinase cascades in gastric mucosal inflammatory responses to Helicobacter pylori lipopolysaccharide. IUBMB Life 51: 315-320, 2001.
33. Kim YH, Lee SH, Lee JY, Choi SW, Park JW and Kwon TK: Triptolide inhibits murine-inducible nitric oxide synthase expression by down-regulating lipopolysaccharide-induced activity of nuclear factor-kappa B and c-Jun NH2-terminal kinase. Eur J Pharmacol 494: 1-9, 2004.
34. Zhou R, Zheng SX, Tang W, He PL, Li XY, Yang YF, Li YC, Geng JG and Zuo JP: Inhibition of inducible nitric-oxide synthase expression by (5R)-5-hydroxytriptolide in interferon-gamma- and bacterial lipopolysaccharide- stimulated macrophages. J Pharmacol Exp Ther 316: 121-128, 2006.
35. Kao CY, Huang F, Chen Y, Thai P, Wachi S, Kim C, Tam L and Wu R: Up-regulation of CC chemokine ligand 20 expression in human airway epithelium by IL-17 through a JAK-independent but MEK/NF-kappaB-dependent signaling pathway. J Immunol 175: 6676-6685, 2005.
36. Miyata Y, Sato T and Ito A: Triptolide, a diterpenoid triepoxide, induces antitumor proliferation via activation of c-Jun NH2-terminal kinase 1 by decreasing phosphatidylinositol 3-kinase activity in human tumor cells. Biochem Biophys Res Commun 336: 1081-1086, 2005.