Antimicrobial peptides as mediators of epithelial host defense

Pediatric Research

Volumn 45, Issue 6, 1999, Pages 785-794

  Huttner, Kenneth M ^a   Bevins, Charles L ^b  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b LERNER RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DEFENSIN; PEPTIDE;

AMINO ACID SEQUENCE; ANTIMICROBIAL ACTIVITY; EPITHELIUM CELL; GENE EXPRESSION; HOST RESISTANCE; LEUKOCYTE; MUCOSAL IMMUNITY; NEWBORN SEPSIS; ONTOGENY; PANETH CELL; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; REVIEW;

EID: 0033044786     PISSN: 00313998     EISSN: None     Source Type: Journal    
DOI: 10.1203/00006450-199906000-00001     Document Type: Review

References (192)

1. Janeway Jr CA, Travers P 1997 Immunobiology: The Immune System in Health and Disease, 3rd Ed. Current Biology, Ltd, London, pp 1.5-1.11
2. Fearon DT 1997 Seeking wisdom in innate immunity. Nature 388:323-324
3. Janeway CA 1989 Approaching the asymptote? Evolution and revolution in immunology. Cold Spring Harbor Symp Quant Biol 54:1-13
4. Fearon DT, Locksley RM 1996 The instructive role of innate immunity in the acquired immune response. Science 272:50-54
5. Mosser 1994 Receptors on phagocytic cells involved in microbial recognition. Immunol Ser 60:99-114
6. Ulevitch RJ, Tobias PS 1994 Recognition of endotoxin by cells leading to transmembrane signaling. Curr Opin Immunol 6:125-130
7. Epstein J, Eichbaum Q, Sheriff S, Ezekowitz RA 1996 The collectins in innate immunity. Curr Opin Immunol 8:29-35
8. Pearson AM 1996 Scavenger receptors in innate immunity. Curr Opin Immunol 8:20-28
9. Medzhitov R, Janeway Jr CA 1997 Innate immunity: the virtues of a nonclonal system of recognition. Cell 91:295-298
10. Janeway CA 1992 The immune system evolved to discriminate infectious nonself from noninfectious self. Immunol Today 13:11-16
11. Wright SD, Unkeless JC 1993 Fatal attraction: recognition and killing mechanisms in innate immunity. Curr Opin Immunol 5:57-58
12. Janeway CA, Bottomly K 1994 Signals and signs for lymphocyte responses. Cell 76:275-285
13. Medzhitov R, Janeway Jr CA 1996 On the semantics of immune recognition. Res Immunol 147:208-214
14. Medzhitov R, Janeway Jr CA 1997 Innate immunity: impact on the adaptive immune response. Curr Opin Immunol 9:4-9
15. Boman HG 1995 Peptide antibiotics and their role in innate immunity. Annu Rev Immunol 13:61-92
16. Hancock REW, Falla T, Brown M 1995 Cationic bactericidal peptides. Adv Microb Physiol 37:135-175
17. Martin E, Ganz T, Lehrer RI 1995 Defensins and other endogenous peptide antibiotics of vertebrates. J Leuk Biol 58:128-136
18. Nissen-Meyer J, Nes IF 1997 Ribosomally synthesized antimicrobial peptides: their function, structure, biogenesis, and mechanism of action. Arch Microbiol 167:67-77
19. Lehrer RI, Bevins CL, Ganz T 1998 Defensins and other antimicrobial peptides. In: Ogra PL, Mestecky J, Lamm ME, Strober WM, Bienstock J (eds) Mucosal Immunology. Academic Press, New York, pp 89-99
20. Boman HG 1998 Gene-encoded peptide antibiotics and the concept of innate immunity: an update review. Scand J Immunol 48:15-25
21. Diamond G, Bevins CL 1998 beta-Defensins: endogenous antibiotics of the innate host defense response. Clin Immunol Immunopathol 88:221-225
22. Lehrer RI, Lichtenstein AK, Ganz T 1993 Defensins: antimicrobial and cytotoxic peptides of mammalian cells. Annu Rev Immunol 11:105-128
23. Lehrer RI, Barton A, Daher KA, Harwig SS, Ganz T, Selsted ME 1989 Interaction of human defensins with Escherichia coli. Mechanism of bactericidal activity. J Clin Invest 84:553-561
24. Kagan BL, Selsted ME, Ganz T, Lehrer RI 1990 Antimicrobial defensin peptides form voltage-dependent ion-permeable channels in planar lipid bilayer membranes. Proc Natl Acad Sci USA 87:210-214
25. Wimley WC, Selsted ME, White SH 1994 Interactions between human defensins and lipid bilayers: evidence for formation of multimeric pores. Protein Sci 3:1362-1373
26. Ludtke SJ, He K, Heller WT, Harroun TA, Yang L, Huang HW 1996 Membrane pores induced by magainin. Biochemistry 43:13723-13728
27. Silvestro L, Gupta K, Weiser JN, Axelsen PH 1997 The concentration-dependent membrane activity of cecropin A. Biochemistry 36:11452-11460
28. Hristova K, Selsted ME, White SH 1996 Interactions of monomeric rabbit neutrophil defensins with bilayers: comparison with dimeric human defensin HNP-2. Biochemistry 35:11888-11894
29. Lohner K, Latal A, Lehrer RI, Ganz T 1997 Differential scanning microcalorimetry indicates that human defensin, HNP-2, interacts specifically with biomembrane mimetic systems. Biochemistry 36:1525-1531
30. Hristova K, Selsted ME, White SH 1997 Critical role of lipid composition in membrane permeabilization by rabbit neutrophil defensins. J Biol Chem 272:24224-24233
31. Boman HG, Agerberth B, Boman A 1993 Mechanisms of action on Escherichia coli of cecropin P1 and PR-39, two antibacterial peptides from pig intestine. Infect Immun 61:2978-2984
32. Falla TJ, Karunartne N, Hancock REW 1996 Mode of action of the antimicrobial peptide indolicidin. J Biol Chem 271:19298-19303
33. Sparkes RS, Kronenberg M, Heinzmann C, Daher KA, Klisak I, Ganz T, Mohandas T 1989 Assignment of defensin gene(s) to human chromosome 8p23. Genomics 5:240-244
34. Ouellette AJ, Pravtcheva D, Ruddle FH, James M 1989 Localization of the cryptdin locus on mouse chromosome 8. Genomics 5:233-239
35. Gudmundsson GH, Magnusson KP, Chowdhary BP, Johansson M, Andersson L, Boman HG 1995 Structure of the gene for porcine peptide antibiotic PR-39, a cathelin gene family member: comparative mapping of the locus for the human peptide antibiotic FALL-39. Proc Natl Acad Sci USA 92:7085-7089
36. Bevins CL, Jones DE, Dutra A, Schaffzin J, Muenke MM 1996 Human enteric defensin genes: chromosomal map position and a model of possible evolutionary relationships. Genomics 31:95-106
37. Iannuzzi L, Gallagher DS, Di Meo GP, Diamond G, Bevins CL, Womack JE 1996 High resolution FISH mapping of beta-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in cattle standard karyotypes. Cytogenet Cell Genet 75:10-13
38. Liu L, Zhao C, Heng HH, Ganz T 1997 The human beta-defensin-1 and alpha-defensins are encoded by adjacent genes: two peptide families with differing disulfide topology share a common ancestry. Genomics 43:316-320
39. Huttner KM, Lambeth MR, Burkin HR, Burkin DJ, Broad TE 1998 Localization and genomic organization of sheep antimicrobial peptide genes. Gene 206:85-91
40. Harder J, Siebert R, Zhang Y, Matthiesen P, Christophers E, Schlegelberger B, Schroder JM 1997 Mapping of the gene encoding human beta-defensin-2 (DEFB2) to chromosome region 8p22-p 23:1. Genomics 46: 472-475
41. Morrison GM, Davidson DJ, Kilanowski FM, Borthwick DW, Crook K, Maxwell AI, Govan JRW, Dorin JR 1998 Mouse beta defensin-1 is a functional homolog of human beta defensin-1. Mamm Genome 9:453-457
42. Diamond G, Jones DE, Bevins CL 1993 Airway epithelial cells are the site of expression of a mammalian antimicrobial peptide gene. Proc Natl Acad Sci USA 90:4596-4600
43. Scocchi M, Wang S, Gennaro R, Zanetti M 1998 Cloning and analysis of a transcript derived from two contiguous genes of the cathelicidin family. Biochim Biophys Acta 1398:393-396
44. Michaeklson D, Rayner J, Couto M, Ganz T 1992 Cationic defensins arise from charge neutralized propeptides: a mechanism for avoiding leukocyte autotoxicity? J Leuk Biol 51:634-639
45. Ganz T, Liu L, Valore E, Oren A 1993 Posttranslational processing and targeting of transgenic human defensin in murine granulocyte, macrophage, fibroblast, pituitary adenoma cell lines. Blood 82:641-650
46. Liu L, Ganz T 1995 The pro region of human neutrophil defensin contains a motif that is essential for normal subcellular sorting. Blood 85:1095-1103
47. Valore EV, Martin E, Harwig SS, Ganz T 1996 Intramolecular inhibition of human defensin HNP-1 by its propiece. J Clin Invest 97:1624-1629
48. Eisenhauer PB, Lehrer RI 1992 Mouse neutrophils lack defensins. Infect Immun 60:3446-3447
49. Levy O 1996 Antibiotic proteins of polymorphonuclear leukocytes. Eur J Haematol 56:263-277
50. Ganz T, Weiss J 1997 Antimicrobial peptides of phagocytes and epithelia. Semin Hematol 34:343-354
51. Bevins CL 1994 Antimicrobial peptides as agents of mucosal immunity. In: Marsh J, Goode JA, Boman H (eds) Antimicrobial Peptides (Ciba Foundation Symposium 186). John Wiley and Sons, Chichester, UK, pp 250-269
52. Ouellette AJ 1997 Paneth cells and innate immunity in the crypt microenvironment. Gastroenterology 113:1779-1784
53. Diamond G, Russell JP, Bevins CL 1996 Inducible expression of an antibiotic peptide gene in lipopolysaccharide-challenged tracheal epithelial cells. Proc Natl Acad Sci USA 93:5156-5160
54. Harder J, Bartels J, Christophers E, Schroder JM 1997 A peptide antibiotic from human skin. Nature 387:861
55. Gallo RL, Ono M, Povsic T, Page C, Eriksson E, Klagsbrun M, Bernfield M 1994 Syndecans, cell surface heparin sulfate proteoglycans, are induced by proline-rich antimicrobial peptide from wounds. Proc Natl Acad Sci USA 91:11035-11039
56. Shi J, Ganz T 1998 The role of protegrins and other elastase-activated polypeptides in the bactericidal properties of porcine inflammatory fluids. Infect Immun 66:3611-3617
57. Soong LB, Ganz T, Ellison A, Caughey GH 1997 Purification and characterization of defensins from cystic fibrosis sputum. Inflamm Res 46:98-102
58. Selsted ME, Harwig SS 1989 Determination of the disulfide array in the human defensin HNP-2. A covalently cyclized peptide. J Biol Chem 264:4003-4007
59. Tang YQ, Selsted ME 1993 Characterization of the disulfide motif in BNBD-12, an antimicrobial beta-defensin peptide from bovine neutrophils. J Biol Chem 268:6649-6653
60. Huttner KM, Kozak CA, Bevins CL 1997 The mouse genome encodes a single homolog of the antimicrobial peptide human beta-defensin 1. FEBS Lett 413:45-49
61. Zimmermann GR, Legault P, Selsted ME, Pardi A 1995 Solution structure of bovine neutrophil beta-defensin-12: the peptide fold of the beta-defensins is identical to that of the classical defensins. Biochemistry 34:13663-13671
62. Pardi A, Hare DR, Selsted ME, Morrison RD, Bassolino DA, Bach II AC 1988 Solution structures of the rabbit neutrophil defensin NP-5. J Mol Biol 201:625-636
63. Hill CP, Yee J, Selsted ME, Eisenberg D 1991 Crystal structure of defensin HNP-3, an amphiphilic dimer: mechanisms of membrane permeabilization. Science 251:1481-1485
64. Rice WG, Ganz T, Kinkade JMJ, Selsted ME, Lehrer RI, Parmley RT 1987 Defensin-rich dense granules of human neutrophils. Blood 70:757-765
65. Gabay JE, Scott RW, Campanelli D, Griffith J, Wilde C, Marra MN, Seeger M, Nathan CF 1989 Antibiotic proteins of human polymorphonuclear leukocytes. Proc Natl Acad Sci USA 86:5610-5614
66. Harwig SSL, Park ASK, Lehrer RI 1992 Characterization of defensin precursors in mature human neutrophils. Blood 79:1532-1537
67. Selsted ME, Miller SI, Henschen AH, Ouellette AJ 1992 Enteric defensins: antibiotic peptide components of intestine host defense. J Cell Biol 118:929-936
68. Porter E, Liu L, Oren A, Anton P, Ganz T 1997 Localization of human intestinal defensin 5 in Paneth cell granules. Infect Immun 65:2389-2395
69. Ganz T, Selsted ME, Szklarek D, Harwig SS, Daher K, Bainton DF, Lehrer RI 1985 Defensins. Natural peptide antibiotics of human neutrophils. J Clin Invest 76:1427-1435
70. Wilde CG, Griffith JE, Marra MN, Snable JL, Scott RW 1989 Purification and characterization of human neutrophil peptide 4, a novel member of the defensin family. J Biol Chem 264:11200-11203
71. Jones DE, Bevins CL 1992 Paneth cells of the human small intestine express an antimicrobial peptide gene. J Biol Chem 267:23216-23225
72. Porter EM, Poles MA, Lee JS, Naitoh J, Bevins CL, Ganz T 1998 Isolation of human intestinal defensins from ileal neobladder urine. FEBS Lett 434:272-276
73. Jones DE, Bevins CL 1993 Defensin-6 mRNA in human Paneth cells: implications for antimicrobial peptides in host defense of the human bowel. FEBS Lett 315:187-192
74. Mallow EB, Harris A, Salzman N, Russell JP, DeBerardinis JR, Ruchelli E, Bevins CL 1996 Human enteric defensins: gene structure and developmental expression. J Biol Chem 271:4038-4045
75. Svinarich DM, Wolf NA, Gomez R, Gonik B, Romero R 1997 Detection of human defensin 5 in reproductive tissues. Am J Obstet Gynecol 176:470-475
76. Svinarich DM, Gomez R, Romero R 1997 Detection of human defensins in the placenta. Am J Reprod Immunol 38:252-255
77. Quayle AJ, Porter EM, Nussbaum AA, Wang YM, Brabec C, Yip KP, Mok SC 1998 Gene expression, immunolocalization, and secretion of human defensin-5 in human female reproductive tract. Am J Pathol 152:1247-1258
78. Valore EV, Ganz T 1992 Posttranslational processing of defensins in immature human myeloid cells. Blood 79:1538-1544
79. Zhang XL, Selsted ME, Pardi A 1992 NMR studies of defensin antimicrobial peptides. 1. Resonance assignment and secondary structure determination of rabbit NP-2 and human HNP-1. Biochemistry 31:11348-11356
80. Lencer WI, Cheung G, Strohmeier GR, Currie MG, Ouellette AJ, Selsted ME, Madara JL 1997 Induction of epithelial chloride secretion by channel-forming cryptins 2 and 3. Proc Natl Acad Sci USA 94:8585-8589
81. Daher KA, Selsted ME, Lehrer RI 1986 Direct inactivation of viruses by human granulocyte defensins. J Virol 60:1068-1074
82. Lehrer RI, Szzklarek D, Ganz T, Selsted ME 1985 Correlation of binding of rabbit granulocyte peptides to Candida albicans: correlation with candacidal activity. Infect Immun 49:207-211
83. Lehrer RI, Szklarek D, Ganz T, Selsted ME 1986 Synergistic activity of rabbit granulocyte peptides against Candida albicans. Infect Immun 52:902-904
84. Lehrer RI, Ganz T, Szklarek D, Selsted ME 1988 Modulation of the in vitro candidacidal activity of human neutrophil defensins by target cell metabolism and divalent cations. J Clin Invest 81:1829-1835
85. Aley SB, Zimmerman M, Hetsko M, Selsted ME, Gillin FD 1994 Killing of Giardia lambia by cryptdins and cationic neutrophil peptides. Infect Immun 62:5397-5403
86. Borenstein LA, Selsted ME, Lehrer RI, Miller JN 1991 Antimicrobial activity of rabbit leukocyte defensins against Treponema pallidum subsp. pallidum. Infect Immun 59:1359-1367
87. Borenstein LA, Selsted ME, Lehrer RI, Miller JN 1991 Contribution of rabbit leukocyte defensins to the host response in experimental syphilis. Infect Immun 59:1368-1377
88. Selsted ME, Tang YQ, Morris WL, McGuire PA, Novotny MJ, Smith W, Henschen AH, Cullor JS 1993 Purification, primary structures, and antibacterial activities of beta-defensins, a new family of antimicrobial peptides from bovine neutrophils. J Biol Chem 268:6641-6648
89. Diamond G, Zasloff M, Eck H, Brasseur M, Maloy WL, Bevins CL 1991 Tracheal antimicrobial peptide, a novel cysteine-rich peptide from mammalian tracheal mucosa: peptide isolation and cloning of a cDNA. Proc Natl Acad Sci USA 88:3952-3956
90. Zhao C, Wang I, Lehrer RI 1996 Widespread expression of beta-defensin hBD-1 in human secretory glands and epithelial cells. FEBS Lett 396:319-322
91. MeCray Jr PB, Bentley L 1997 Human airway epithelia express a beta-defensin. Am J Respir Cell Mol Biol 16:343-349
92. Stolzenberg ED, Anderson GM, Ackermann MR, Whitlock RH, Zasloff M 1997 Epithelial antibiotic induced in states of disease. Proc Nail Acad Sci USA 94:8686-8690
93. Schonwetter BS, Stolzenberg ED, Zasloff MA 1995 Epithelial antibiotics induced at sites of inflammation. Science 267:1645-1648
94. Zhang G, Wu H, Shi J, Ganz T, Ross CR, Blecha F 1998 Molecular cloning and tissue expression of porcine beta-defensin-1. FEBS Lett 424:37-40
95. Bensch KW, Raida M, Magert HJ, Schulz-Knappe P, Forssmann WG 1995 hBD-1: a novel β-defensin from human plasma. FEBS Lett 368:331-335
96. Valore EV, Park CH, Quayle AJ, Wiles KR, McCray Jr PB, Ganz T 1998 Human β-defensin-1: an antimicrobial peptide of urogenital tissues. J Clin Invest 101:1633-1642
97. Tarver AP, Clark DP, Diamond G, Cohen KM, Erdjument-Bromage H, Jones DE, Sweeney R, Wines M, Hwang S, Tempst P, Bevins CL 1998 Enteric β-defensin: molecular cloning and characterization of a gene with inducible intestinal epithelial expression associated with Cryptospiridium parvum expression. Infect Immun 66:1045-1056
98. Huttner KM, Brezinski-Caliguri DJ, Mahoney MM, Diamond G 1998 Antimicrobial peptide expression is developmentally regulated in the ovine gastrointestinal tract. J Nutr 128:297S-299S
99. Harwig SSL, Swiderek KM, Kokryakov VN, Tan L, Lee TD, Panyutich EA, Aleshina GM, Shamova OV, Lehrer RI 1994 Gallinacins: cysteine-rich antimicrobial peptides of chicken leukocytes. FEBS Lett 342:281-285
100. Lawyer C, Pai S, Watabe M, Bakir H, Eagleton L, Watabe K 1996 Effects of synthetic form of tracheal antimicrobial peptide on respiratory pathogens. J Antimicrob Chemother 37:599-604
101. Bals R, Goldman MJ, Wilson JM 1998 Mouse beta-defensin 1 is a salt-sensitive antimicrobial peptide present in epithelia of the lung and urogenital tract. Infect Immun 66:1225-1232
102. Russell JP, Diamond G, Tarver A, Bevins CL 1996 Coordinate induction of two antibiotic genes in tracheal epithelial cells exposed to the inflammatory mediators lipopolysaccharide and tumor necrosis factor-α. Infect Immun 64:1565-1568
103. Krisanaprakornkit S, Weinberg A, Perez CN, Dale BA 1998 Expression of the peptide antibiotic human beta-defensin 1 in cultured gingival epithelial cells and gingival tissue. Infect Immun 66:4222-4228
104. Schnapp D, Harris A 1998 Antibacterial peptides in bronchoalveolar lavage fluid. Am J Respir Cell Mol Biol 19:352-356
105. Bals R, Wang X, Wu Z, Freeman T, Bafna V, Zasloff M, Wilson JM 1998 Human β-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung. J Clin Invest 102:874-880
106. Hiratsuka T, Nakazato M, Date Y, Ashitani J, Minematsu T, Chino N, Matsukura S 1998 Identification of human beta-defensin-2 in respiratory tract and plasma and its increase in bacterial pneumonia. Biochem Biophys Res Commun 249:943-947
107. Singh PK, Jia HP, Wiles K, Hesselberth J, Liu L, Conway BA, Greenberg EP, Valore EV, Welsh MJ, Ganz T, Tack BF, McCray Jr PB 1998 Production of beta-defensins by human airway epithelia. Proc Natl Acad Sci USA 95:14961-14966
108. Zanetti M, Gennaro R, Romeo D 1995 Catheticidins: a novel protein family with a common proregion and a variable C-terminal antimicrobial domain. FEBS Lett 374:1-5
109. Zanetti M, Del Sal G, Storici P, Schneider C, Romeo D 1993 The cDNA of the neutrophil antibiotic BAC5 predicts a pro-sequence homologous to a cysteine proteinase inhibitor that is common to other neutrophil antibiotics. J Biol Chem 268:522-526
110. Levy O, Weiss J, Zarember K, Ooi CE, Eisbach P 1993 Antibacterial 15-kDa protein isoforms (p15s) are members of a novel family of leukocyte proteins. J Biol Chem 268:6058
111. Mahoney MM, Lee AY, Brezinski-Caliguri DJ, Huttner KM 1995 Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide. FEBS Lett 377:519-522
112. Zanetti M, Litteri L, Gennaro R, Horstmann H, Romeo D 1990 Bactenecins, defense polypeptides of bovine neutrophils, are generated from precursor molecules stored in the large granules. J Cell Biol 111:1363-1371
113. Storici P, Tossi A, Lenarcic B, Romeo D 1996 Purification and structural characterization of bovine cathelicidins, precursors of antimicrobial peptides. Eur J Biochem 238:769-776
114. Scocchi M, Skerlavaj B, Romeo D, Gennaro R 1992 Proteolytic cleavage by neutrophil elastase converts inactive storage preforms to antibacterial bactenecins. Eur J Biochem 209:589-595
115. Panyutich A, Shi J, Boutz PL, Zhao C, Ganz T 1997 Porcine polymorphonuclear leukocytes generate extracellular microbicidal activity by elastase-mediated activation of secreted proprotegrins. Infect Immun 65:978-985
116. Storici P, Del Sal G, Schneider C, Zanetti M 1992 cDNA sequence analysis of an antibiotic dodecapeptide from neutrophils. FEBS Lett 314:187-190
117. Del Sal G, Storici P, Schneider C, Romeo D, Zanetti M 1992 cDNA cloning of the neutrophil bactericidal peptide indolicidin. Biochem Biophys Res Commun 187:467-472
118. Storici P, Zanetti M 1993 A cDNA derived from pig bone marrow cells predicts a sequence identical to the intestinal antibacterial peptide PR-39. Biochem Biophys Res Commun 196:1058-1065
119. Storici P, Zanetti M 1993 A novel cDNA sequence encoding a pig leukocyte antimicrobial peptide with a cathelin-like pro-sequence. Biochem Biophys Res Commun 196:1363-1368
120. Zhao C, Liu L, Lehrer RI 1994 Identification of a new member of the protegrin family by cDNA cloning. FEBS Lett 346:285-288
121. Zanetti M, Storici P, Tossi A, Scocchi M, Gennaro R 1994 Molecular cloning and chemical synthesis of a novel antibacterial peptide derived from pig myeloid cells. J Biol Chem 269:7855-7858
122. Scocchi M, Romeo D, Zanetti M 1994 Molecular cloning of Bac7, a proline- and arginine-rich antimicrobial peptide from bovine neutrophils. FEBS Lett 352:197-200
123. Storici P, Scocchi M, Tossi A, Gennaro R, Zanetti M 1994 Chemical synthesis and biological activity of a novel antibacterial peptide deduced from a pig myeloid cDNA. FEBS Lett 337:303-307
124. Zhao C, Ganz T, Lehrer RI 1995 The structure of porcine protegrin genes. FEBS Lett 368:197-202
125. Bagella L, Scocchi M, Zanetti M 1995 cDNA sequences of three sheep myeloid cathelicidins. FEBS Lett 376:225-228
126. Tossi A, Scocchi M, Zanetti M, Storici P, Gennaro R 1995 PMAP-37, a novel antibacterial peptide from pig myeloid cells. cDNA cloning, chemical synthesis and activity. Eur J Biochem 228:941-946
127. Zhuo C, Ganz T, Lehrer RI 1995 Structures of genes for two cathelin-associated antimicrobial peptides: prophenin-2 and PR-39. FEBS Lett 376:130-134
128. Skerlavaj B, Gennaro R, Bagella L, Merluzzi L, Risso A, Zanctti M 1996 Biological characterization of two novel cathelicidin-derived peptides and identification of structural requirements for their antimicrobial and cell lytic activities. J Biol Chem 271:28375-28381
129. Scocchi M, Wang S, Zanetti M 1997 Structural organization of the bovine cathelicidin gene family and identification of a novel member. FEBS Lett 417:311-315
130. Agerberth B, Gunne H, Odeberg J, Kogner P, Boman HG, Gudmundsson GH 1995 FALL-39, a putative human peptide antibiotic, is cysteine-tree and expressed in bone marrow and testis. Proc Natl Acad Sci USA 92:195-199
131. Gallo RL, Kim KJ, Berntield M, Kozak CA, Zanetti M, Merluzzi L, Gennaro R 1997 Identification of CRAMP, a cathelin-related antimicrobial peptide expressed in the embryonic and adult mouse. J Biol Chem 272:13088-13093
132. Gudmundsson G, Agerberth B, Odeberg J, Bergman T, Olsson B, Salcedo R 1996 The human gene FALL39 and processing of the cathelin precursor to the antibacterial peptide LL-37 in granulocytes. Eur J Biochem 238:325-332
133. Bals R, Wang X, Zasloff M, Wilson JM 1998 The peptide antibiotic LL-37/hCAP-18 is expressed in epithelia of the human lung where it has broad antimicrobial activity at the airway surface. Proc Natl Acad Sci USA 95:9541-9546
134. Frohm M, Agerbenh B, Ahangari G, Stahle-Backdahl M, Liden S, Wigzell H, Gudmundsson GH 1997 The expression of the gene coding for the antibacterial peptide LL-37 is induced in human keratinoeytes during inflammatory disorders. J Biol Chem 272:15258-15263
135. Yeaman MR, Tang YQ, Shen AJ, Bayer AS, Selsled ME 1997 Purification and in vitro activities of rabbit platelet microbicidal proteins. Infect Immun 65:1023-1031
136. Xu T, Levitz SM, Diamond RD, Oppenheim FG 1991 Anticandidial activity of major human salivary histatins. Infect Immun 59:2549-2554
137. Schlievert P, Johnson W, Galask RP 1976 Isolation of a low-molecular-weight antibacterial system from human amniotic fluid. Infect Immun 14:1156-1166
138. Brogden KA, De Lucca AJ, Bland J, Elliott S 1996 Isolation of an ovine pulmonary surfactant-associated anionic peptide bactericidal for Pasteurella haemolytica. Proc Natl Acad Sci USA 93:412-416
139. Brogden KA, Ackermann M, Huttner KM 1997 Small, anionic, and charge-neutralising propeptide fragments of zymogens are antimicrobial. Antimicrob Agents Cnemother 41:1615-1617
140. Ganz T, Rayner JR, Valore EV, Tumolo A, Talmadge K, Fuller F 1989 The structure of the rabbit macrophage defensin genes and their organ-specific expression. J Immunol 143:1358-1365
141. Ouellette AJ, Cordell B 1988 Accumulation of abundant messenger ribonucleic acids during postnatal development of mouse small intestine. Gastroenterology 94:114-121
142. Ouellette AJ, Greco RM, James M, Frederick D, Naftilan J, Fallon JT 1989 Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium. J Cell Biol 108:1687-1695
143. Darmoul D, Brown D, Selsted ME, Ouellette AJ 1997 Cryptdin gene expression in developing mouse small intestine. Am J Physiol 272:G197-G206
144. Levy O, Ooi CE, Weiss J, Lehrer RI, Elsbach P 1994 Individual and synergistic effects of rabbit granulocyte proteins on Escherichia coli. J Clin Invest 94:672-682
145. Westerhoff HV, Zasloff M, Rosner JL, Hendler RW, DeWaal A, VazGomez A, Jongsma APM, Riethorst A, Juretic D 1995 Functional synergism of the magainins PGLa and magainin-2 in E. coli, tumor cells, and liposomes. Eur J Biochem 228:257-264
146. Panyutich AV, Hiemstra PS, van Wetering S, Ganz T 1995 Human neutrophil defensin and serpins form complexes and inactivate each other. Am J Respir Cell Mol Biol 12:351-357
147. Territo MC, Ganz T, Selsled ME, Lehrer R 1989 Monoeyte-chemotactic activity of defensins from human neutrophils. J Clin Invest 84:2017-2020
148. Huang HJ, Ross CR, Bleeha F 1997 Chemoattractant properties of PR-39, a neutrophil antibacterial peptide. J Leukoc Biol 61:624-629
149. Chenov O, Michiel DF, Xu L, Wang JM, Tani K, Murphy WJ, Longo DL, Taub DD, Oppenheim JJ 1996 Identification of defensin-1, defensin-2, and CAP37/azurocidin as T-cell chemoattractant proteins released from interleukin-8-stimulated neutrophils. J Biol Chem 271:2935-2940
150. Murphy CJ, Foster BA, Mannis MJ, Selsted ME, Reid TW 1993 Defensins are mitogenic for epithelial cells and fibroblasts. J Cell Physiol 155:408-413
151. Zhu Q, Singh AV, Bateman A, Esch F, Solomon S 1987 The corticostatic (anti-ACTH) and cytotoxic activity of peptides isolated from fetal, adult, and tumor-bearing lung. J Ster Biochem 27:1017-1022
152. Singh A, Bateman A, Zhu QZ, Shimasaki S, Esch F, Solomon S 1988 Structure of a novel human granulocyte peptide with anti-ACTH activity. Bioch Biophys Res Commun 155:524-529
153. Higazi AAR, Ganz T, Kariko K, Cines DB 1996 Defensin modulates tissue-type plasminogen activator and plasminogen binding to fibrin and endothelial cells. J Biol Chem 271:17650-17655
154. Higazi AA, Lavi E, Bdeir K, Ulrich AM, Jamieson DG, Rader DJ, Usher DC, Kane W, Ganz T, Cines DB 1997 Defensin stimulates the binding of lipoprotein (a) to human vascular endothelial and smooth muscle cells. Blood 89:4290-4298
155. Zhao C, Liaw L, Lee IH, Lehrer RI 1997 cDNA cloning of Clavanins: antimicrobial peptides of tunicate hemocytes. FEBS Lett 410:490-492
156. Zhao C, Liaw L, Lee IH, Lehrer RI 1997 cDNA cloning of three cecropin-like antimicrobial peptides (Styelins) from the tunicate. Styela clava. FEBS Lett 412:144-148
157. Salzman NH, Polin RA, Harris MC, Ruchelli E, Hebra A, Zirin-Butler S, Jawad A, Martin Porter E, Bevins CL 1998 Enteric defensin expression in necrotizing enterocolitis. Pediatr Res 44:20-26
158. Groisman EA, Chiao E, Lipps CJ, Heffron F 1989 Salmonella typhimurium phoP virulence gene is a transcriptional regulator. Proc Natl Acad Sci USA 86:7077-7081
159. Miller SI, Pulkkinen WS, Selsted ME, Mekalanos JJ 1990 Characterization of defensin resistance phenotypes associated with mutations in the phoP virulence regulon of Salmonella typhinurium. Infect Immun 58:3706-3710
160. Gunn JS, Miller SI 1996 PhoP-PhoQ activates transcription of pmrAB, encoding a two-component regulatory system involved in Salmonella typhimurium antimicrobial peptide resistance. J Bacteriol 178:6857-6864
161. Porter E, van Dam E, Valore E, Ganz T 1997 Broad spectrum antimicrobial activity of human intestinal defensin 5. Infect Immun 65:2396-2401
162. Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA 1996 The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in drosophila adults. Cell 86:973-983
163. Hoffmann JA, Reichart JM 1997 Drosophila immunity. Trends Cell Biol 7:309-316
164. Epple P, Apel K, Bohlmann H 1997 Overexpression of an endogenous thionin enhances resistance of Arabidopsis against Fusarium oxysporum. Plant Cell 9:509-520
165. Davis PB, Drumm M, Konstan MW 1996 Cystic fibrosis. Am J Respir Crit Care Med 154:1229-1256
166. Smith JJ, Travis SM, Greenberg EP, Welsh MJ 1996 Cystic fibrosis airway epithelia fail to kill bacteria because of abnormal airway surface fluid. Cell 85:229-236
167. Goldman MJ, Anderson GM, Stolzenberg ED, Kari UP, Zasloff M, Wilson JM 1997 Human beta-defensin-1 is a salt-sensitive antibiotic in lung that is inactivated in cystic fibrosis. Cell 88:553-560
168. Palfree RG, Sadro LC, Solomon S 1993 The gene encoding the human corticostatin HP-4 precursor contains a recent 86-base duplication and is located on chromosome 8. Mol Endocrinol 7:199-205
169. Ganz T, Selsted ME, Lehrer RI 1990 Defensins. Eur J Haematol 44:1-8
170. Mars WM, Stellrecht CM, Stass S, Frazier ML, Saunders GF 1987 Localization of an abundant myeloid mRNA to individual leukocytes in mixed cell populations. Leukemia 1:167-172
171. Auphan N, DiDonato JA, Rosette C, Helmberg A, Karin M 1995 Immunosuppression by glucocorticoids: inhibition of NF-kB activity through induction of IkB synthesis. Science 270:286-290
172. Simmaco M, Boman A, Mangoni ML, Mignogna G, Miele R, Barra D, Boman HG 1997 Effect of glucocorticoids on the synthesis of antimicrobial peptides in amphibian skin. FEBS Lett 416:273-275
173. Kelley KJ 1996 Using host defenses to fight infectious diseases. Nature Biotech 14:587-590
174. Spitznagel JK 1997 Origins and development of peptide antibiotic research. From extracts to abstracts to contracts. Methods Mol Biol 78:1-14
175. Selsted ME, Brown DM, DeLange RJ, Harwig SSL, Lehrer RI 1985 Primary structures of six antimicrobial peptides of rabbit peritoneal neutrophils. J Biol Chem 260:4579-4585
176. Ryan LK, Rhodes J, Bhat M, Diamond G 1996 Expression of beta-defensin genes in bovine alveolar macrophages. Infect Immun 66:878-881
177. Ouellette AJ, Lualdi JC 1990 A novel mouse gene family coding for cationic, cysteine-rich peptides. J Biol Chem 265:9831-9837
178. Fulton C, Anderson GM, Zasloff M, Bull R, Quinn AG 1997 Expression of natural peptide antibiotics in human skin. Lancet 350:1750-1751
179. Ouellette AJ, Miller SI, Henschen AH, Selsted ME 1992 Purification and primary structure of murine cryptdin-1, a Paneth cell defensin. FEBS Lett 304:146-148
180. Eisenhauer PB, Harwig SSSL, Lehrer RI 1992 Cryptdins: antimicrobial defensins of the murine small intestine. Infect Immun 60:3556-3565
181. Bateman A, MacLeod RJ, Lembessis P, Hu J, Esch F, Solomon S 1996 The isolation and characterization of a novel corticostatin/defensin-like peptide from the kidney. J Biol Chem 271:10654-10659
182. Cowland JB, Johnsen AH, Borregaard N 1995 hCAP-18, a cathelin/pro-bactenecin-like protein of human neutrophil specific granules. FEBS Lett 368:173-176
183. Romeo D, Skerlavaj B, Bolognesi M, Gennaro R 1988 Structure and bactericidal activity of an antibiotic dodecapeptide purified from bovine neutrophils. J Biol Chem 263:9573-9575
184. Gennaro R, Skerlavaj B, Romeo D 1989 Purification, composition, and activity of two bactenecins, antibacterial peptides of bovine neutrophils. Infect Immun 57:3142-3146
185. Selsted ME, Novotny MJ, Morris WJ, Tang YQ, Smith W, Cullor JS 1992 Indolicidin, a novel bactericidal tridecapeptide amide from neutrophils. J Biol Chem 267:4292-4295
186. Tossi A, Scocchi M, Skerlavaj B, Gennaro R 1994 Identification and characterization of a primary antibacterial domain in CAP18, a lipopolysaccharide binding protein from rabbit leukocytes. FEBS Lett 339:108-112
187. Agerberth B, Lee JY, Bergman T, Carlquist M, Boman HG, Mutt V, Jörnvall H 1991 Amino acid sequence of PR-39, isolation from pig intestine of a new member of the family of proline-arginine-rich antibacterial peptides. Eur J Biochem 202:849-854
188. Kokryakov VN, Harwig SS, Panyutich EA, Shevchenko AA, Aleshina GM, Shamova OV, Korneva HA, Lehrer RI 1993 Protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins. FEBS Lett 315:231-236
189. Pungercar J, Strukelj B, Kopitar G, Renko M, Lenarcic B, Gubensek F, Turk V 1993 Molecular cloning of a putative homolog of proline/arginine-rich antibacterial peptides from porcine bone marrow. FEBS Lett 336:284-288
190. Harwig SS, Kokryakov VN, Swiderek KM, Aleshina GM, Zhao C, Lehrer RI 1995 Prophenin-1, an exceptionally proline-rich antimicrobial peptide from porcine leukocytes. FEBS Lett 362:65-69
191. Harwig SS, Waring A, Yang HJ, Cho Y, Tan L, Lehrer RI 1996 Intramolecular disulfide bonds enhance the antimicrobial and lytic activities of protegrins at physiological sodium chloride concentrations. Eur J Biochem 240:352-357
192. Selsted ME, Harwig SS, Ganz T, Schilling JW, Lehrer RI 1985 Primary structures of three human neutrophil defensins. J Clin Invest 76:1436-1439