Highly sensitive DNA detection and point mutation identification: An electrochemical approach based on the combined use of ligase and reverse molecular beacon

Human Mutation

Volumn 28, Issue 6, 2007, Pages 630-637

  Wu, Zai Sheng ^a   Jiang, Jian Hui ^a   Shen, Guo Li ^a   Yu, Ru Qin ^a  

^a HUNAN UNIVERSITY   (China)

Author keywords

AC voltammogram; E. coli DNA ligase; Point mutation identification; Reverse molecular beacon

Indexed keywords

3' FERROCENE; 5' PHOSPHORYL; DNA; FERROCENE DERIVATIVE; LIGASE; OLIGONUCLEOTIDE; PHOSPHORUS; UNCLASSIFIED DRUG;

ARTICLE; DNA CONFORMATION; DNA DETERMINATION; DNA SEQUENCE; DNA STRAND; ELECTROCHEMICAL ANALYSIS; ELECTRODE; GENE IDENTIFICATION; MOLECULAR BEACON; POINT MUTATION; PRIORITY JOURNAL; PROTEIN STRUCTURE;

BIOSENSING TECHNIQUES; DNA; DNA LIGASES; ELECTROCHEMISTRY; ELECTRODES; FERROUS COMPOUNDS; GOLD; NUCLEIC ACID HYBRIDIZATION; PERCHLORIC ACID; POINT MUTATION; SENSITIVITY AND SPECIFICITY; SODIUM COMPOUNDS;

EID: 34248353386     PISSN: 10597794     EISSN: None     Source Type: Journal    
DOI: 10.1002/humu.20487     Document Type: Article

References (52)

1. Alfonta L, Singh AK, Willner I. 2001. Liposomes labeled with biotin and horseradish peroxidase: a probe for the enhanced amplification of antigen-antibody or oligonucleotide-DNA sensing processes by the precipitation of an insoluble product on electrodes. Anal Chem 73:91-102.
2. Anne A, Bouchardon A, Moiroux J. 2003. 3′-ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces: novel model systems for exploring flexibility of short DNA using cyclic voltammetry. J Am Chem Soc 125:1112-1113.
3. Anne A, Demaille C. 2006. Dynamics of electron transport by elastic bending of short DNA duplexes. Experimental study and quantitative modeling of the cyclic voltammetric behavior of 3′-ferrocenyl DNA end-grafted on gold. J Am Chem Soc 128:542-557.
4. Baker BR, Lai RY, Wood MS, Doctor EH, Heeger AJ, Plaxco KW. 2006. An electronic, aptamer-based small-molecule sensor for the rapid, label-free detection of cocaine in adulterated samples and biological fluids. J Am Chem Soc 128:3138-3139.
5. Boon EM, Ceres DM, Drummond TG, Hill MG, Barton JK. 2000. Mutation detection by electrocatalysis at DNA-modified electrodes. Nat Biotechnol 18:1096-1100.
6. Cooper MA, Dultsev FN, Minson T, Ostanin VP, Abell C, Klenerman D. 2001. Direct and sensitive detection of a human virus by rupture event scanning. Nat Biotechnol 19:833-837.
7. Creager S, Yu CJ, Bamdad C, O'Connor S, MacLean T, Lam E, Chong Y, Olsen GT, Luo J, Gozin M, Kayyem JF. 1999. Electron transfer at electrodes through conjugated "molecular wire" bridges. J Am Chem Soc 121:1059-1064.
8. Culha M, Stokes D, Allain LR, Vo-Dinh T. 2003. Surface-enhanced raman scattering substrate based on a self-assembled monolayer for use in gene diagnostics. Anal Chem 75:6196-6201.
9. Dong T-Y, Chang LS, Tseng IM, Huang SJ. 2004. Electroactive self-assembled biferrocenyl alkanethiol monolayers on Au(111) surface and on gold nanoclusters. Langmuir 20:4471-4479.
10. Du H, Strohsahl CM, Miller BL, Krauss TD. 2005. Sensitivity and specificity of metal surface-immobilized "molecular beacon" biosensors. J Am Chem Soc 127:7932-7940.
11. Fan CH, Plaxco KW, Heeger AJ. 2003. Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA. Proc Natl Acad Sci USA 100:9134-9137.
12. Galasso K, Livache T, Roget A, Vieil E. 1998. Electrogravimetric detection of DNA hybridization on polypyrrole copolymer. J Chim Phys 95:1514-1517.
13. Gibbs JM, Park S-J, Anderson DR, Watson KJ, Mirkin CA, Nguyen ST. 2005. Polymer-DNA hybrids as electrochemical probes for the detection of DNA. J Am Chem Soc 127:1170-1178.
14. Herne TM, Tarlov MJ. 1997. Characterization of DNA probes immobilized on gold surfaces. J Am Chem Soc 119:8916-8920.
15. Immoos CE, Lee SJ, Grinstaff MW. 2004a. Electrochemical interrogation of conformational changes as a reagentless method for the sequence-specific detection of DNA. Chembiochem 5:1100-1103.
16. Immoos CE, Lee SJ, Grinstaff MW. 2004b. DNA-PEG-DNA triblock macromolecules for reagentless DNA detection. J Am Chem Soc 126:10814-10815.
17. Jordan CE, Frutos AG, Thiel AJ, Corn RM. 1997. Surface plasmon resonance imaging measurements of DNA hybridization adsorption and streptavidin/DNA multilayer formation at chemically modified gold surfaces. Anal Chem 69:4939-4947.
18. Kelley SO, Boon EM, Barton JK, Jackson NM, Hill MG. 1999. Single-base mismatch detection based on charge transduction through DNA. Nucleic Acids Res 27:4830-4837.
19. Kerman K, Saito M, Morita Y, Takamura Y, Ozsoz M, Tamiya E. 2004. Electrochemical coding of single-nucleotide polymorphisms by monobase-modified gold nanoparticles. Anal Chem 76:1877-1884.
20. Kertesz V, Whittemore NA, Chambers JQ, McKinney MS, Baker DC. 2000. Surface titration of DNA-modified gold electrodes with a thioltethered anthraquinone. J Electroanal Chem 493:28-36.
21. Kim E, Kim K, Yang H, Kim YT, Kwak J. 2003. Enzyme-amplified electrochemical detection of DNA using electrocatalysis of ferrocenyltethered dendrimer. Anal Chem 75:5665-5672.
22. Kim K, Yang H, Park SH, Lee DS, Kim SJ, Lim YT, Tae Y. 2004. Washingfree electrochemical DNA detection using double-stranded probes and competitive hybridization reaction. Chem Chem Commun 13:1466-1467.
23. Lai RY, Seferos DS, Heeger AJ, Bazan GC, Plaxco KW. 2006. Comparison of the signaling and stability of electrochemical DNA sensors fabricated from 6- or 11-carbon self-assembled monolayers. Langmuir 22:10796-10800.
24. Le Floch F, Ho HA, Leclerc M. 2006. Label-free electrochemical detection of protein based on a ferrocene-bearing cationic polythiophene and aptamer. Anal Chem 78:4727-4731.
25. Leavy MC, Bhattacharyya S, Cleland WE Jr, Hussey CL. 1999. Electrochemical and spectroscopic characterization of self-assembled monolayers of unsymmetrical ferrocenyl dialkyl sulfide derivatives on gold. Langmuir 15:6582-6586.
26. Levicky R, Herne TM, Tarlov MJ, Satija SK. 1998. Using self-assembly to control the structure of DNA monolayers on gold: a neutron reflectivity study. J Am Chem Soc 120:9787-9792.
27. Li J, Chu X, Liu Y, Jiang JH, He Z, Zhang Z, Shen G, Yu RQ. 2005. A colorimetric method for point mutation detection using high-fidelity DNA ligase. Nucleic Acids Res 33:e168.
28. Liu B, Bard AJ, Mirkin MV, Creager SE. 2004. Electron Transfer at self-assembled monolayers measured by scanning electrochemical microscopy. J Am Chem Soc 126:1485-1492.
29. Liu G, Lee TMH, Wang J. 2005a. Nanocrystal-based bioelectronic coding of single nucleotide polymorphisms. J Am Chem Soc 127:38-39.
30. Liu J, Tian S, Tiefenauer L, Nielsen PE, Knoll W. 2005b. Simultaneously amplified electrochemical and surface plasmon optical detection of DNA hybridization based on ferrocene-streptavidin conjugates. Anal Chem 77:2756-2761.
31. O'Connor SD, Olsen GT, Creager SE. 1999. A Nernstian electron source model for the AC voltammetric response of a reversible surface redox reaction using large-amplitude AC voltages. J Electroanal Chem 466:197-202.
32. Ozkan D, Erdem A, Kara P, Kerman K, Meric B, Hassmann J, Ozsoz M. 2002. Allele-specific genotype detection of Factor V Leiden mutation from polymerase chain reaction amplicons based on label-free electrochemical genosensor. Anal Chem 74:5931-5936.
33. Park SJ, Taton TA, Mirkin CA. 2002. Array-based electrical detection of DNA with nanoparticle probes. Science 295:1503-1506.
34. Peterson AW, Heaton JR, Geogiadis RM. 2001. The effect of surface probe density on DNA hybridization. Nucleic Acids Res 29:5163-5168.
35. Peterson AW, Wolf LK, Georgiadis RM. 2002. Hybridization of mismatched or partially matched DNA at surfaces. J Am Chem Soc 124:14601-14607.
36. Piuuno PAE, Krull UJ, Hudson RHE, Damha MJ, Cohen H. 1995. Fiber-optic DNA sensor for fluorometric nucleic acid determination. Anal Chem 67:2635-2643.
37. Radi A-E, Acero Sanchez JL, Baldrich E, O'Sullivan CK. 2006. Reagentless, reusable, ultrasensitive electrochemical molecular beacon aptasensor. J Am Chem Soc 128:117-124.
38. Sato S, Kondo H, Nojima T, Takenaka S. 2005. Electrochemical telomerase assay with ferrocenylnaphthalene diimide as a tetraplex DNA-specific binder. Anal Chem 77:7304-7309.
39. Smalley JF, Finklea HO, Chidsey CED, Linford MR, Creager SE, Ferraris JP, Chalfant K, Zawodzinsk T, Feldberg SW, Newton MD. 2003. Heterogeneous electron-transfer kinetics for ruthenium and ferrocene redox moieties through alkanethiol monolayers on gold. J Am Chem Soc 125:2004-2013.
40. Su H, Kallury KMR, Thompson M. 1994. Interfacial nucleic acid hybridization studied by random primer 32P labeling and liquid-phase acoustic network analysis. Anal Chem 66:769-777.
41. Su L, Sankar CG, Sen D, Yu H-Z. 2004. Kinetics of ion-exchange binding of redox metal cations to thiolate-DNA monolayers on gold. Anal Chem 76:5953-5959.
42. Sumner JJ, Creager SE. 2001. Redox kinetics in monolayers on electrodes: electron transfer is sluggish for ferrocene groups buried within the monolayer interior. J Phys Chem B 105:8739-8745.
43. Tansil NC, Xie H, Xie F, Gao Z. 2005. Direct detection of DNA with an electrocatalytic threading intercalator. Anal Chem 77:126-134.
44. Wang J, Li J, Baca AJ, Hu J, Zhou F, Yan W, Pang D-W. 2003. Amplified voltammetric detection of DNA hybridization via oxidation of ferrocene caps on gold nanoparticle/streptavidin conjugates. Anal Chem 75:3941-3945.
45. Wang J, Liu G, Merkoci A. 2003. electrochemical coding technology for simultaneous detection of multiple DNA targets. J Am Chem Soc 125:3214-3215.
46. Weizmann Y, Patolsky F, Willner I. 2001. Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticles. Analyst 126:1502-1504.
47. Whittemore NA, Mullenix AN, Inamati GB, Manoharan M, Cook PD, Tuinman AA, Baker DC, Chambers JQ. 1999. Synthesis and electrochemistry of anthraquinone-oligodeoxynucleotide conjugates. Bioconjugate Chem 10:261-270.
48. Wong ELS, Gooding JJ. 2003. Electronic detection of target nucleic acids by a 2,6-disulfonic acid anthraquinone intercalator. Anal Chem 75:3845-3852.
49. Xiao Y, Piorek BD, Plaxco KW, Heeger AJ. 2005. A reagentless signal-on architecture for electronic, aptamer-based sensors via target-induced strand displacement. J Am Chem Soc 127:17990-17991.
50. Yu CJ, Wan Y, Yowanto H, Li J, Tao C, James MD, Tan CL, Blackburn GF, Meade TJ. 2001. Electronic detection of single-base mismatches in DNA with ferrocene-modified probes. J Am Chem Soc 123:11155-11161.
51. 2 as the substrate. Anal Chem 76:4093-4097.
52. Zhou XC, O'Shea SJ, Li SFY. 2000. Amplified microgravimetric gene sensor using Au nanoparticle modified oligonucleotides. Chem Commun 11:953-954.