메뉴 건너뛰기




Volumn 127, Issue 4, 2005, Pages 1170-1178

Polymer-DNA hybrids as electrochemical probes for the detection of DNA

Author keywords

[No Author keywords available]

Indexed keywords

BLOCK COPOLYMERS; ELECTROCHEMISTRY; MELTING; RING OPENING POLYMERIZATION; SYNTHESIS (CHEMICAL);

EID: 13644278966     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja046931i     Document Type: Article
Times cited : (147)

References (41)
  • 29
    • 13644277138 scopus 로고    scopus 로고
    • note
    • Based on the molar absorptivity of DNA at 287 nm and the diphenylacetylene spacer at 287 and 307 nm, we determined that there are, on average, five DNA strands per single polymer chain, which translates to ∼30% occupation of the 17 available DNA coupling sites on the poly2 block. This value was calculated by first determining the polymer concentration based on the absorbance at 307 nm, which is only due to the polymer component and not the DNA. Knowing the polymer concentration and its molar absorptivity at 287 nm allowed us to subtract the polymers' absorbance at 287 nm from the total absorbance. The DNA concentration was determined from the remaining absorbance at 287 nm and the molar absorptivity value at 287 for the DNA sequences. The DNA concentration divided by the polymer concentration gives the average value of DNA per polymer.
  • 40
    • 13644281598 scopus 로고    scopus 로고
    • note
    • Consider the theoretical case of triblock copolymers with a maximum number of 5 repeating units per each electrochemically active block, distinct ratios which can be observed electrochemically would consist of the following: (0:5), (1:3), (1:4), (1:5), (2:3), (2:4), (2:5), (3:1), (3:2), (3:4), (3:5), (4:1), (4:2), (4:3), (4:5), (5:0), (5:1), (5:2), (5:3), (5:4), (5:5).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.