Characterization of DNA probes immobilized on gold surfaces

Journal of the American Chemical Society

Volumn 119, Issue 38, 1997, Pages 8916-8920

  Herne, Tonya M ^a   Tarlov, Michael J ^a  

^a NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GOLD; SINGLE STRANDED DNA;

ADSORPTION; ARTICLE; DNA PROBE; HYBRIDIZATION; IMMOBILIZATION; ROENTGEN SPECTROSCOPY; SPECTROSCOPY; SURFACE PROPERTY;

EID: 0030825711     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9719586     Document Type: Article

References (20)

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16. Certain commercial products and instruments are identified to adequately specify the experimental procedure. In no case does such identification imply endorsement by the National Institute of Standards and Technology.
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18. Herne, T. M.; Tarlov, M. J. We have determined, using XPS and P radiolabeling techniques, that ssDNA molecules will not adsorb on MCH SAMs (unpublished results).
19. The samples in the SPR experiment were prepared by forming the HS-ssDNA monolayer on gold over a period of 4-5 h, followed by a 14 h exposure to 1 μM MCH. The longer posttreatment in MCH may result in a less-than-optimized coverage of HS-ssDNA.
20. +)) + 0.41(% G + C) - 0.61 (% formamide present) - 500/L, where L = the length of the shortest chain in the duplex.