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+ T cell lineage commitment. Nat Immunol 6 (2005) 793-799. This study demonstrates that the memory phenotype of cells after infection is determined by precursor cell number. It also provides a warning that some adoptive transfer studies may be skewed by using an unphysiological number of T cells.
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This study demonstrates that a structurally 'featureless' influenza A-derived epitope engages a restricted TCR repertoire, whereas a repertoire with greater diversity is selected by an epitope with more prominent determinants. Importantly, a mutation in the latter epitope that reduced side chain accessibility was associated with a commensurate reduction in the diversity of the cognate repertoire on infection with a virus engineered to express this variant.
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+ T cell populations. Nat Immunol 6 (2005) 382-389. This study demonstrates that a structurally 'featureless' influenza A-derived epitope engages a restricted TCR repertoire, whereas a repertoire with greater diversity is selected by an epitope with more prominent determinants. Importantly, a mutation in the latter epitope that reduced side chain accessibility was associated with a commensurate reduction in the diversity of the cognate repertoire on infection with a virus engineered to express this variant.
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Huseby E.S., Crawford F., White J., Marrack P., and Kappler J.W. Interface-disrupting amino acids establish specificity between T cell receptors and complexes of major histocompatibility complex and peptide. Nat Immunol 7 (2006) 1191-1199. This paper demonstrates that cross-reactive TCRs bind fewer pMHC side chains with high affinity than more specific TCRs. In addition, it reports the intriguing observation that 'interface-disruptive' side chains contribute to TCR specificity by exerting inhibitory effects on binding.
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