메뉴 건너뛰기




Volumn 32, Issue 2, 2003, Pages 265-275

Functional expression and direct visualization of the human α 2B-adrenergic receptor and α2B-AR-green fluorescent fusion protein in mammalian cell using Semliki Forest virus vectors

Author keywords

CHO cells; Fusion protein; GPCR; Localization; Over expression; Semliki Forest virus

Indexed keywords

MAMMALIA; RABBIT FIBROMA VIRUS; SEMLIKI FOREST VIRUS;

EID: 0348196650     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.08.006     Document Type: Article
Times cited : (16)

References (39)
  • 1
    • 0023728920 scopus 로고
    • Receptors linked to inhibition of adenylate cyclase: Additional signaling mechanisms
    • L.E. Limbird, Receptors linked to inhibition of adenylate cyclase: additional signaling mechanisms, FASEB J. 2 (1988) 2686-2695.
    • (1988) FASEB J. , vol.2 , pp. 2686-2695
    • Limbird, L.E.1
  • 3
    • 0025821645 scopus 로고
    • 2-adrenergic receptor subtypes and G proteins in reconstituted phospholipid vesicles
    • 2-adrenergic receptor subtypes and G proteins in reconstituted phospholipid vesicles, Biochemistry 30 (1991) 3335-3341.
    • (1991) Biochemistry , vol.30 , pp. 3335-3341
    • Kurose, H.1    Regan, J.W.2    Caron, M.G.3    Lefkowitz, R.J.4
  • 5
  • 6
    • 0036490096 scopus 로고    scopus 로고
    • Regulators of G-protein signalling as new central nervous system drug targets
    • R.R. Neubig, D.P. Sidervoski, Regulators of G-protein signalling as new central nervous system drug targets, Nat. Rev. Drug Discov. 1 (2002) 187-197.
    • (2002) Nat. Rev. Drug Discov. , vol.1 , pp. 187-197
    • Neubig, R.R.1    Sidervoski, D.P.2
  • 8
    • 0027966894 scopus 로고
    • High-level expression of human neurokinin-1 receptor in mammalian cell line using Semliki Forest virus expression system
    • K. Lundstrom, A. Mills, G. Buell, E. Allet, N. Adami, P. Liljestrom, High-level expression of human neurokinin-1 receptor in mammalian cell line using Semliki Forest virus expression system, Eur. J. Biochem. 224 (1994) 917-921.
    • (1994) Eur. J. Biochem. , vol.224 , pp. 917-921
    • Lundstrom, K.1    Mills, A.2    Buell, G.3    Allet, E.4    Adami, N.5    Liljestrom, P.6
  • 10
    • 0033066197 scopus 로고    scopus 로고
    • 1b-Adrenergic receptor and G-protein subunits in mammalian cell lines using the Semliki Forest virus expression system
    • 1b-Adrenergic receptor and G-protein subunits in mammalian cell lines using the Semliki Forest virus expression system, J. Recept. Signal Transduct. Res. 19 (1999) 369-378.
    • (1999) J. Recept. Signal Transduct. Res. , vol.19 , pp. 369-378
    • Scheer, A.1    Björklöf, K.2    Cotecchia, S.3    Lundstrom, K.4
  • 11
    • 0034469508 scopus 로고    scopus 로고
    • Alphavirus vectors: Applications for DNA vaccine production and gene expression
    • K. Lundstrom, Alphavirus vectors: applications for DNA vaccine production and gene expression, Intervirology 43 (2001) 247-257.
    • (2001) Intervirology , vol.43 , pp. 247-257
    • Lundstrom, K.1
  • 12
    • 0032998543 scopus 로고    scopus 로고
    • Alphaviruses as tools in neurobiology and gene therapy
    • K. Lundstrom, Alphaviruses as tools in neurobiology and gene therapy, J. Recept. Signal Transduct. Res. 19 (1999) 673-686.
    • (1999) J. Recept. Signal Transduct. Res. , vol.19 , pp. 673-686
    • Lundstrom, K.1
  • 14
    • 0034193130 scopus 로고    scopus 로고
    • Using green fluorescent proteins to study G-protein-coupled receptor localization and trafficking
    • L. Kallal, J.L. Benovic, Using green fluorescent proteins to study G-protein-coupled receptor localization and trafficking, Trends Pharmacol. Sci. 21 (2000) 175-180.
    • (2000) Trends Pharmacol. Sci. , vol.21 , pp. 175-180
    • Kallal, L.1    Benovic, J.L.2
  • 15
    • 0032699862 scopus 로고    scopus 로고
    • Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein
    • G. Milligan, Exploring the dynamics of regulation of G protein-coupled receptors using green fluorescent protein, Br. J. Pharmacol. 128 (1999) 501-510.
    • (1999) Br. J. Pharmacol. , vol.128 , pp. 501-510
    • Milligan, G.1
  • 16
    • 0036151483 scopus 로고    scopus 로고
    • Real-time visualization of a fluorescent Gαs: Dissociation of the activated G Protein from plasma membrane
    • J.-Z. Yu, M.M. Rasenick, Real-time visualization of a fluorescent Gαs: Dissociation of the activated G Protein from plasma membrane, Mol. Pharmacol. 61 (2002) 352-359.
    • (2002) Mol. Pharmacol. , vol.61 , pp. 352-359
    • Yu, J.-Z.1    Rasenick, M.M.2
  • 17
    • 14744304517 scopus 로고
    • A new generation of animal cell expression vectors based on Semliki Forest virus replicon
    • P. Liljestrom, H. Garoff, A new generation of animal cell expression vectors based on Semliki Forest virus replicon. Bio/Technology 9 (1991) 1356-1361.
    • (1991) Bio/Technology , vol.9 , pp. 1356-1361
    • Liljestrom, P.1    Garoff, H.2
  • 18
    • 0002840787 scopus 로고    scopus 로고
    • J. Crawley, C. Gerfen, R. McKay, M. Rogawski, D. Sibley, P. Skolnick (Eds.), Alphavirus-mediated gene transfer into neurons, Wiley, New York
    • M.U. Ehrengruber, K. Lundstrom, in: J. Crawley, C. Gerfen, R. McKay, M. Rogawski, D. Sibley, P. Skolnick (Eds.), Alphavirus-mediated gene transfer into neurons, Curr. Prot. Neurosci., Wiley, New York, 2000, pp 4.22.1-4.22.23.
    • (2000) Curr. Prot. Neurosci. , pp. 4221-42223
    • Ehrengruber, M.U.1    Lundstrom, K.2
  • 19
    • 0028152741 scopus 로고
    • A significantly improved Semliki Forest virus expression system based on the translation enhancer segments from the viral capsid gene
    • E.M. Sjöberg, M. Suomalainen, H. Garoff, A significantly improved Semliki Forest virus expression system based on the translation enhancer segments from the viral capsid gene, Bio/Technology 12 (1994) 1127-1131.
    • (1994) Bio/Technology , vol.12 , pp. 1127-1131
    • Sjöberg, E.M.1    Suomalainen, M.2    Garoff, H.3
  • 20
    • 0035903167 scopus 로고    scopus 로고
    • Phenoxybenzamine binding reveals the helical orientation of the third transmembrane domain of the adrenergic receptors
    • H. Frang, V. Cockcroft, T. Karskela, M. Scheinin, A. Marjamäki, Phenoxybenzamine binding reveals the helical orientation of the third transmembrane domain of the adrenergic receptors, J. Biol. Chem. 276 (2001) 31279-31284.
    • (2001) J. Biol. Chem. , vol.276 , pp. 31279-31284
    • Frang, H.1    Cockcroft, V.2    Karskela, T.3    Scheinin, M.4    Marjamäki, A.5
  • 21
    • 0032423267 scopus 로고    scopus 로고
    • Effect of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures
    • E.-J. Schlaeger, K. Lundstrom, Effect of temperature on recombinant protein expression in Semliki Forest virus infected mammalian cell lines growing in serum-free suspension cultures, Cytotechnology 28 (1998) 205-211.
    • (1998) Cytotechnology , vol.28 , pp. 205-211
    • Schlaeger, E.-J.1    Lundstrom, K.2
  • 24
    • 0014949207 scopus 로고
    • Cleavage of structural proteins during the assembly of the head of bacteriophage T4
    • U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227 (1970) 680-685.
    • (1970) Nature , vol.227 , pp. 680-685
    • Laemmli, U.K.1
  • 25
    • 0009482260 scopus 로고
    • Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
    • H. Towbin, T. Staehelin, J. Gordon, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl. Acad. Sci. USA 76 (1979) 4350-4354.
    • (1979) Proc. Natl. Acad. Sci. USA , vol.76 , pp. 4350-4354
    • Towbin, H.1    Staehelin, T.2    Gordon, J.3
  • 26
  • 27
    • 0022509767 scopus 로고
    • Quantitation in immunochemistry: Correlation of immunogold labeling to absolute number of membrane antigens
    • G. Griffiths, H. Hoppeler, Quantitation in immunochemistry: correlation of immunogold labeling to absolute number of membrane antigens, J. Histochem. Cytochem. 34 (1986) 1389-1398.
    • (1986) J. Histochem. Cytochem. , vol.34 , pp. 1389-1398
    • Griffiths, G.1    Hoppeler, H.2
  • 29
    • 0026476534 scopus 로고
    • Quantitation of gold labeling and estimation of labeling efficiency with a stereological counting method
    • J. Lucocq, Quantitation of gold labeling and estimation of labeling efficiency with a stereological counting method, J. Histochem. Cytochem. 40 (1992) 1929-1936.
    • (1992) J. Histochem. Cytochem. , vol.40 , pp. 1929-1936
    • Lucocq, J.1
  • 32
    • 0037048801 scopus 로고    scopus 로고
    • Green fluorescent protein as a reporter of human μ-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris
    • V. Sarramegna, F. Talmont, M. Seree de Roch, A. Milon, P. Demange, Green fluorescent protein as a reporter of human μ-opioid receptor overexpression and localization in the methylotrophic yeast Pichia pastoris, J. Biotech. 99 (2002) 23-39.
    • (2002) J. Biotech. , vol.99 , pp. 23-39
    • Sarramegna, V.1    Talmont, F.2    Seree De Roch, M.3    Milon, A.4    Demange, P.5
  • 33
    • 0034787010 scopus 로고    scopus 로고
    • Crystallization of membrane proteins in cubo
    • P. Nollert, J. Navarro, E.M. Landau, Crystallization of membrane proteins in cubo, Methods Enzymol. 343 (2002) 183-199.
    • (2002) Methods Enzymol. , vol.343 , pp. 183-199
    • Nollert, P.1    Navarro, J.2    Landau, E.M.3
  • 34
    • 0028061573 scopus 로고
    • 3H]MK912 is alpha-2C selective among human alpha-2A, alpha-2B and alpha-2C adrenoceptors
    • 3H]MK912 is alpha-2C selective among human alpha-2A, alpha-2B and alpha-2C adrenoceptors, J. Pharmacol. Exp. Ther. 271 (1994) 1558-1565.
    • (1994) J. Pharmacol. Exp. Ther. , vol.271 , pp. 1558-1565
    • Uhlen, S.1    Porter, A.C.2    Neubig, R.R.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.