Directed evolution of Tk-subtilisin from a hyperthermophilic archaeon: Identification of a single amino acid substitution responsible for low-temperature adaptation

Protein Engineering, Design and Selection

Volumn 20, Issue 3, 2007, Pages 143-153

  Pulido, M A ^a   Koga, Y ^a   Takano, K ^a,b   Kanaya, S ^a  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Directed evolution; Low temperature adaptation; Propeptide; Subtilisin; Thermococcus kodakaraensis

Indexed keywords

DEGRADATION; GENES; MUTAGENESIS; PROTEINS; SUBSTITUTION REACTIONS; SYNTHESIS (CHEMICAL);

DIRECTED EVOLUTION; LOW TEMPERATURE ADAPTATION; PROPEPTIDE; SUBTILISIN; THERMOCOCCUS KODAKARAENSIS;

AMINO ACIDS;

BACTERIAL PROTEIN; MUTANT PROTEIN; PEPTIDE; PROTEIN PRECURSOR; SUBTILISIN;

AMINO ACID SUBSTITUTION; ARTICLE; DISSOCIATION; HYPERTHERMOPHILIC ARCHAEON; LOW TEMPERATURE; MUTAGENESIS; MUTATION; MUTATIONAL ANALYSIS; NUCLEOTIDE SEQUENCE; PEPTIDE ANALYSIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN SECRETION; PROTEIN STABILITY; PROTEIN STRUCTURE; SCREENING; STRUCTURE ANALYSIS; TEMPERATURE ACCLIMATIZATION; THERMOCOCCUS; THERMOCOCCUS KODAKARAENSIS; THERMOSTABILITY;

AMINO ACID SUBSTITUTION; DIRECTED MOLECULAR EVOLUTION; PROTEIN PRECURSORS; SUBTILISIN; TEMPERATURE; THERMOCOCCUS;

ARCHAEA; THERMOCOCCUS KODAKARENSIS;

EID: 34047216653     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzm006     Document Type: Article

References (48)

1. Arnold,F.H. (1996) Chem. Eng. Sci., 51, 5091-5102.
2. Arnold,F.H., Wintrode,P.L., Miyazaki,K. and Gershenson,A. (2001) Trends Biochem. Sci., 26, 100-106.
3. Bloom,J.D., Meyer,M.M., Meinhold,P., Otey,C.R., MacMillan,D. and Arnold,F.H. (2005) Curr. Opin. Struct. Biol., 15, 447-452.
4. Bryan,P.N. (2000) Biochim. Biophys. Acta, 1543, 203-222.
5. Bryan,P.N., Rollence,M., Pantoliano,M., Wood,J., Finzel,B., Gilliland,G.L., Howard,A. and Poulos,T. (1986) Proteins, 1, 326-334.
6. Chen,K. and Arnold,F.H. (1993) Proc. Natl Acad. Sci. USA, 90, 5618-5622.
7. Cherry,J.R. and Fidantsef,A.L. (2003) Curr. Opin. Biotechnol., 14, 438-443.
8. Dyson,H.J. and Wright,P.E. (2005) Nat. Rev. Mol. Cell. Biol., 6, 197-208.
9. Eder,J., Rheinnecker,M. and Fersht,A.R. (1993a) Biochemistry, 32, 18-26.
10. Eder,J., Rheinnecker,M. and Fersht,A.R. (1993b) J. Mol. Biol., 233, 293-304.
11. Eijsink,V.G., Gaseidnes,S., Borchert,T.V. and van den Burg,B. (2005) Biomol. Eng., 22, 21-30.
12. Farinas,E., Butler,T. and Arnold,F.H. (2001) Curr. Opin. Biotechnol., 12, 545-551.
13. Fu,X., Inouye,M. and Shinde,U. (2000) J. Biol. Chem., 275, 16871-16878.
14. Gallagher,T., Bryan,P.N. and Gilliland,G.L. (1993) Proteins, 16, 205-213.
15. Gallagher,T., Gilliland,G.L., Wang,L. and Bryan,P.N. (1995) Structure, 3, 907-914.
16. Goodwin,T.W. and Morton,R.A. (1946) Biochem. J., 40, 628-632.
17. Huang,H.W., Chen,W.C., Wu,C.Y., Yu,H.C., Lin,W.Y., Chen,S.T. and Wang,K.T. (1997) Protein Eng., 10, 1227-1233.
18. Hu,Z., Haghjoo,K. and Jordan,F. (1996) J. Biol. Chem., 271, 3375-3384.
19. Jain,S.C., Shinde,U., Li,Y., Inouye,M. and Berman,H.M. (1998) J. Mol. Biol., 284, 137-144.
20. Kannan,Y., Koga,Y., Inoue,Y., Haruki,M., Takagi,M., Imanaka,T., Morikawa,M. and Kanaya,S. (2001) Appl. Environ. Microbiol., 67, 2445-2452.
21. Kano,H., Taguchi,S. and Momose,H. (1997) Appl. Microbiol. Biotechnol., 47, 46-51.
22. Kobayashi,T. and Inouye,M. (1992) J. Mol. Biol., 226, 931-933.
23. Kojima,S., Yanai,H. and Miura,K. (2001) J. Biochem. (Tokyo), 130, 471-474.
24. Lerner,C., Kobayashi,T. and Inouye,M. (1990) J. Biol. Chem., 265, 20085-20086.
25. Li,Y., Hu,Z., Jordan,F. and Inouye,M. (1995) J. Biol. Chem., 270, 25127-25132.
26. Marie-Claire,Y., Yabuta,Y., Suefuji,K., Matsuzawa,H. and Shinde,U. (2001) J. Mol. Biol., 305, 151-165.
27. Pulido,M., Saito,K., Tanaka,S., Koga,Y., Takano,K. and Kanaya,S. (2006). Appl. Environ. Microbiol., 72, 4154-4162.
28. Shinde,U. and Inouye,M. (2000) Semin. Cell Dev. Biol., 11, 35-44.
29. Shinde,U., Liu,J. and Inouye,M. (1997) Nature, 389, 520-522.
30. Shinde,U., Li,Y., Chatterjee,S. and Inouye,M. (1993) Proc. Natl Acad. Sci. USA, 90, 6924-6928.
31. Song,J.K. and Rhee,J.S. (2000) Appl. Environ. Microbiol., 66, 890-894.
32. Srimathi,S., Jayaraman,G. and Narayanan,P.R. (2006) Enzyme Microb. Tech., 39, 301-307.
33. Strausberg,S.L., Alexander,P.A., Gallagher,D.T., Gilliland,G.L., Barnett,B.L. and Bryan,P.N. (1995) Biotechnology, 13, 669-673.
34. Subbian,E., Yabuta,Y. and Shinde,U. (2005) J. Mol. Biol., 347, 367-383.
35. Taguchi,S., Komada,S. and Momose,H. (2000) Appl. Environ. Microbiol., 66, 1410-1415.
36. Taguchi,S., Ozaki,A. and Momose,H. (1998) Appl. Environ. Microbiol., 64, 492-495.
37. Takagi,H. and Takahashi,M. (2003) Appl. Microbiol. Biotechnol., 63, 1-9.
38. Tao,H. and Cornish,V. (2002) Curr. Opin. Chem. Biol., 6, 858-864.
39. Tompa,P. (2002) Trends Biochem. Sci., 27, 527-533.
40. Wang,L., Ruan,B., Ruvinov,S. and Bryan,P.N. (1998) Biochemistry, 37, 3165-3171.
41. Wang,L., Ruvinov,S., Strausberg,S., Gallagher,D.T., Gilliland,G.L. and Bryan,P.N. (1995) Biochemistry, 34, 15415-15420.
42. Wintrode,P., Miyazaki,K. and Arnold,F.H. (2001) Biochim. Biophys. Acta, 1549, 1-8.
43. Yabuta,Y., Subbian,E., Oiry,C. and Shinde,U. (2003) J. Biol. Chem., 278, 15246-15251.
44. Yabuta,Y., Subbian,E., Takagi,H., Shinde,U. and Inouye,M. (2002) J. Biochem. (Tokyo), 131, 31-37.
45. Yabuta,Y., Takagi,H., Inouye,M. and Shinde,U. (2001) J. Biol. Chem., 276, 44427-44434.
46. Yang,Y., Jiang,L., Zhu,L., Wu,Y. and Yang,S. (2000) J. Biotechnol., 81, 113-118.
47. You,L. and Arnold,F.H. (1994) Protein Eng., 9, 77-83.
48. Zhao,H. and Arnold,F.H. (1999) Protein Eng., 12, 47-53.