Effects of capsaicin on induction of apoptosis and inhibition of adipogenesis in 3T3-L1 cells

Journal of Agricultural and Food Chemistry

Volumn 55, Issue 5, 2007, Pages 1730-1736

  Hsu, Chin Lin ^a   Yen, Gow Chin ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

3T3 L1 cells; Adipogenesis; Apoptosis; Capsaicin; Protein expression

Indexed keywords

CAPSAICIN;

ADIPOCYTE; ADIPOGENESIS; ANIMAL; APOPTOSIS; ARTICLE; CELL DIVISION; CELL STRAIN 3T3; DRUG EFFECT; METABOLISM; MOUSE; STEM CELL;

3T3-L1 CELLS; ADIPOCYTES; ADIPOGENESIS; ANIMALS; APOPTOSIS; CAPSAICIN; CELL DIVISION; MICE; STEM CELLS;

EID: 33947596710     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf062912b     Document Type: Article

References (45)

1. Kopelman, P. G. Obesity as a medical problem. Nature 2000, 404, 635-643.
2. Formiguera, X.; Canton, A. Obesity: epidemiology and clinical aspects. Best Pract. Res. Clin. Gastroenterol. 2004, 18, 1125-1146.
3. Wang, Y. W.; Jones, P. J. Conjugated linolic acid and obesity control: efficacy and mechanisms. Int. J. Obes. Relat. Metab. Disord. 2004, 28, 941-955.
4. Furuyashiki, T.; Nagayasu, H.; Aoki, Y.; Bessho, H.; Hashimoto, T.; Kanazawa, K.; Ashida, H. Tea catechin suppresses adipocyte differentiation accompanied by down-regulation of PPARγ2 and C/EBPα in 3T3-L1 cells. Biosci. Biotechnol. Biochem. 2004, 68, 2353-2359.
5. Hausman, D. B.; DiGirolamo, M.; Bartness, T. J.; Hausman, G. J.; Martin, R. J. The biology of white adipocyte proliferation. Obes. Rev. 2001, 2, 239-254.
6. MacDougald, O. A.; Mandrup, S. Adipogenesis: forces that tip the scales. Trends Endocrinol. Metab. 2002, 13, 5-11.
7. Cordell, G. A.; Araujo, O. E. Capsaicin: identification, nomenclature, and pharmacotherapy. Ann. Pharmacother. 1993, 27, 330-336.
8. Surh, Y. J. Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem. Toxicol. 2002, 40, 1091-1097.
9. Yoshioka, M.; St-Pierre, S.; Drapeau, V.; Dionne, I.; Doucet, E.; Suzuki, M.; Tremblay, A. Effects of red pepper on appetite and energy intake. Br. J. Nutr. 1999, 82, 115-123.
10. Kawada, T.; Hagihara, K. I.; Iwai, K. Effects of capsaicin on lipid metabolism in rats fed a high fat diet. J. Nutr. 1986a, 116, 1272-1278.
11. Morre, D. J.; Chueh, P. J.; Morre, D. M. Capsaicin inhibits preferentially the NADH oxidase and growth of transformed cells in culture. Proc. Natl. Acad. Sci. U.S.A. 1995, 92, 1831-1835.
12. Macho, A.; Sancho, R.; Minassi, A.; Appendino, G.; Lawen, A.; Munoz, E. Involvement of reactive oxygen species in capsaicinoid-induced apoptosis in transformed cells. Free Radical Res. 2003, 37, 611-619.
13. Vermeulen, K.; Van Bockstaele, D. R.; Berneman, Z. N. Apoptosis: mechanisms and relevance in cancer. Ann. Hematol. 2005, 84, 627-639.
14. Woo, K. J.; Jeong, Y. J.; Park, J. W.; Kwon, T. K. Chrysin-induced apoptosis is mediated through caspase activation and Akt inactivation in U937 leukemia cells. Biochem. Biophys. Res. Commun. 2004, 325, 1215-1222.
15. Fan, T. J.; Han, L. H.; Cong, R. S.; Liang, J. Caspase family proteases and apoptosis. Acta Biochim. Biophys. Sin. 2005, 37, 719-727.
16. Hsu, C. L.; Huang, S. L.; Yen, G. C. Inhibitory effect of phenolic acids on proliferation of 3T3-L1 preadipocytes in relation to their antioxidant activity. J. Agric. Food Chem. 2006, 54, 4191-4197.
17. Green, H.; Kehinde, O. Sublines of mouse 3T3 cells that accumulate lipid. Cell 1974, 1, 113-116.
18. Mosmann, T. Rapid coiorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.
19. Takada, E.; Toyota, H.; Suzuki, J.; Mizuguchi, J. Prevention of anti-IgM-induced apoptosis accompanying G1 arrest in B lymphoma cells overexpressing dominant-negative mutant form of c-Jun N-terminal kinase 1. J. Immunol. 2001, 166, 1641-1649.
20. Ramirez-Zacarias, J. L.; Castro-Munozledo, F.; Kuri-Harcuch, W. Quantitation of adipose conversion and triglycerides by staining intercytoplasmic lipids with Oil Red O. Histochemistry 1992, 97, 493-497.
21. Wise, L. S.; Green, H. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. J. Biol. Chem. 1979, 254, 273-275.
22. Cohen, G. M. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16.
23. Proter, A. G.; Janicke, R. U. Emerging roles of caspase-3 on apoptosis. Cell Death Differ. 1999, 6, 99-104.
24. Antonsson, B.; Martinou, J. C. The bcl-2 protein family. Exp. Cell Res. 2000, 256, 50-57.
25. Tontonoz, P.; Hu, E.; Graves, R. A.; Budavari, A. I.; Spiegelman, B. M. mPPARγ2: tissue-specific regulator of an adipocyte enhancer. Genes Dev. 1994, 8, 1224-1234.
26. Bray, G. A.; Tartaglia, L. A. Medicinal strategies in the treatment of obesity. Nature 2000, 404, 672-677.
27. Cowherd, R. M.; Lyle, R. E.; McGehee, R. E., Jr. Molecular regulation of adipocyte differentiation. Cell Dev. Biol. 1999, 10, 3-10.
28. Saltiel, A. R.; Kahn, C. R. Insulin signaling and the regulation of glucose and lipid metabolism. Nature 2001, 414, 799-806.
29. Wang, Y. W.; Jones, P. J. Conjugated linolic acid and obesity control: efficacy and mechanisms. Int. J. Obes. Relat. Metab. Disord. 2004, 28, 941-955.
30. Vermes, I.; Haanen, C.; Steffens-Nakken, H.; Reutelingsperger, C. A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J. Immunol. Methods 1995, 184, 39-51.
31. Raghuvar Gopal, D. V.; Narkar, A. A.; Badrinath, Y.; Mishra, K. P.; Joshi, D. S. Protection of Ewing's sarcoma family tumor (ESFT) cell line SK-N-MC from betulinic acid induced apoptosis by α-DL-tocopherol. Toxicol. Lett. 2004, 153, 201-212.
32. Li, P.; Nijhawan, D.; Budihardjo, I.; Srinivasula, S. M.; Ahmad, M.; Alnemri, E. S.; Wang, X. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997, 91, 479-489.
33. Lazebnik, Y. A.; Kaufmann, S. H.; Desnoyers, S.; Poirer, G. G.; Earnshaw, W. C. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 1994, 371, 346-347.
34. Jung, M. Y.; Kang, H. J.; Moon, A. Capsaicin-induced apoptosis in SK-Hep-1 hepatocarcinoma cells involves Bcl-2 downregulation and caspase-3 activation. Cancer Lett. 2001, 165, 139-145.
35. Yang, J. Y.; Della-Fera, M. A.; Baile C. A. Esculetin induces mitochondria-mediated apoptosis in 3T3-L1 adipocytes. Apoptosis 2006, 11, 1371-1378.
36. Poulos, S. P.; Sisk, M.; Hausman, D. B.; Azain, M. J.; Hausman, G. J. Pre- and postnatal dietary conjugated linoleic acid alters adipose development, body weight gain and body composition in sprague-dawley rats. J. Nutr. 2001, 131, 2722-2731.
37. Mochizuki, M.; Hasegawa, N. Effects of green tea catechin-induced lipolysis on cytosol glycerol content in differentiated 3T3-L1 cells. Phytother. Res. 2004, 18, 945-946.
38. Mori, M.; Hasegawa, N. Superoxide dismutase activity enhanced by green tea inhibits lipid accumulation in 3T3-L1 cells. Phytother. Res. 2003, 17, 566-567.
39. Takahata, T.; Kumano, T.; Ookawa, K.; Hayakari, M.; Kakizaki, I.; Tsuchida, S. Inhibition of 3T3-L1 adipocyte differentiation by 6-ethoxyzolamide: repressed peroxisome proliferator-activated receptor γ mRNA and enhanced CCAAT/enhancer binding protein β mRNA levels. Biochem. Pharmacol. 2004, 67, 1667-1675.
40. Kersten, S.; Desvergne, B.; Wahli, W. Roles of PPARs in health and disease. Nature 2000, 405, 421-424.
41. Pajvani, U. B.; Du, X.; Combs, T. P.; Berg, A. H.; Rajala, M. W.; Schulthess, T.; Engel, J.; Brownlee, M.; Scherer, P. E. Structure-function studies of the adipocyte-secreted hormone Acrp30/adiponectin. Implications fpr metabolic regulation and bioactivity. J. Biol. Chem. 2003, 278, 9073-9085.
42. Ahima, R. S.; Flier, J. S. Leptin. Annu. Rev. Physiol. 2000, 62, 413-437.
43. Jeon, T.; Hwang, S. G.; Hirai, S.; Matsui, T.; Yano, H.; Kawada, T.; Lim, B. O.; Park, D. K. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci. 2004, 75, 3195-3203.
44. Rumsfield, J. A.; West, D. P. Topical capsaicin in dermatologic and peripheral pain disorders. Ann. Pharmacother. 1991, 125, 381-387.
45. Hwang, J. T.; Park, I. J.; Shin, J. I.; Lee, Y. K.; Lee, S. K.; Baik, H. W.; Ha, J.; Park, O. J. Genistein, EGCG, and capsaicin inhibit adipocyte differentiation process via activating AMP-activated protein kinase. Biochem. Biophys. Res. Commun. 2005, 338, 694-699.