Cloning and high-level production of a chitinase from Chromobacterium sp. and the role of conserved or nonconserved residues on its catalytic activity

Applied Microbiology and Biotechnology

Volumn 74, Issue 4, 2007, Pages 791-804

  Park, Seur Kee ^a   Kim, Chi Wook ^b   Kim, Hoon ^a   Jung, Jae Sung ^a   Harman, G E ^c  

^a Sunchon National University   (South Korea)

^b Dong Bang Agro Corp   (South Korea)

^c Department of Obstetrics Gynecology   (United States)

Author keywords

Chitinase; Enzyme enhancement; Enzyme purification; Site directed mutagenesis

Indexed keywords

AMINO ACIDS; BACTERIA; CATALYST ACTIVITY; CLONING; GENES; MUTAGENESIS;

CHITINASE; ENZYME ENHANCEMENT; ENZYME PURIFICATION; SITE-DIRECTED MUTAGENESIS;

ENZYMES;

CHITINASE;

AMINO ACID; BACTERIUM; CATALYSIS; CELLULOSE; CHITIN; CLONE; ENZYME ACTIVITY; GENE; MUTATION;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BINDING AFFINITY; CATALYSIS; CHROMOBACTERIUM; ENZYME ACTIVITY; ENZYME BINDING; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE SEQUENCE; GENETIC CONSERVATION; ISOELECTRIC POINT; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DOMAIN; SITE DIRECTED MUTAGENESIS; SUPERNATANT; WILD TYPE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BASE SEQUENCE; CATALYTIC DOMAIN; CELLULOSE; CHITIN; CHITINASE; CHROMOBACTERIUM; CLONING, MOLECULAR; CONSERVED SEQUENCE; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; MUTATION; PROTEIN BINDING; PROTEIN SORTING SIGNALS; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

CHROMOBACTERIUM SP; ESCHERICHIA COLI;

EID: 33847320652     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0614-0     Document Type: Article

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