Functional analysis of the carbohydrate-binding domains of Erwinia chrysanthemi Cel5 (endoglucanase Z) and an Escherichia coli putative chitinase

Journal of Bacteriology

Volumn 181, Issue 15, 1999, Pages 4611-4616

  Simpson, Helen D ^a   Barras, Frederic ^a,b  

^a CNRS   (France)

^b AIX MARSEILLE UNIVERSITÉ   (France)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CARBOHYDRATE BINDING PROTEIN; CHITINASE; GLUCAN SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; DELETION MUTANT; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME CONFORMATION; ERWINIA; ESCHERICHIA COLI; HYDROGEN BOND; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

BACTERIA (MICROORGANISMS); ERWINIA; ERWINIA CHRYSANTHEMI; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0032793044     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.15.4611-4616.1999     Document Type: Article

References (38)

1. Andrews, S. C., P. M. Harrison, and J. R. Guest. 1989. Cloning, sequencing, and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12. J. Bacteriol. 171:3940-3947.
2. Ansaldi, M., M. Lepelletier, and V. Mejean. 1996. Site-specific mutagenesis by using an accurate recombinant polymerase chain reaction method. Anal. Biochem. 234:110-111.
3. Barras, F., I. Bortoli-German, M. Bauzan, J. Rouvier, C. Gey, A. Heyraud, and B. Henrissat. 1992. Stereochemistry of the hydrolysis reaction catalysed by endoglucanase Z from Erwinia chrysanthemi. FEBS Lett. 300:145-148.
4. Blaak, H., J. Schnellmann, S. Walter, B. Henrissat, and H. Schrempf. 1993. Characteristics of an exochitinase from Streptomyces olivaceoviridis, its corresponding gene, putative protein domains and relationship to other chitinases. Eur. J. Biochem. 214:659-669.
5. Boisset, C. Personal communication.
6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem, 72:248-254.
7. Brun, E., P. Gans, D. Marion, and F. Barras. 1995. Overproduction, purification and characterisation of the cellulose-binding domain of the Erwinia chrysanthemi secreted endoglucanase EGZ. Eur. J. Biochem. 231:142-148.
8. Brun, E., F. Moriaud, P. Gans, M. J. Blackledge, F. Barras, and D. Marion. 1997. Solution structure of the cellulose-binding domain of the endoglucanase Z secreted by Erwinia chrysanthemi. Biochemistry 36:16074-16086.
9. Chung, C. T., and R. H. Miller. 1988. A rapid and convenient method for the preparation and storage of competent bacterial cells. Nucleic Acids Res. 16:3580.
10. Coutinho, J. B., N. R. Gilkes, R. A. Warren, D. G. Kilburn, and R. C. Miller. 1992. The binding of Cellulomonas fimi endoglucanase C (CenC) to cellulose and Sephadex is mediated by the N-terminal repeats. Mol. Microbiol. 6:1243-1253.
11. Fontes, C. M., J. H. Clarke, G. P. Hazlewood, T. H. Fernandes, T. H. Gilbert, and L. M. Ferreira. 1998. Identification of tandemly repeated type VI cellulose-binding domains in an endoglucanase from the aerobic soil bacterium Cellvibrio mixtus. Appl. Microbiol. Biotechnol. 49:552-559.
12. Fujii, T., and K. Miyashita. 1993. Multiple domain structure in a chitinase gene (chiC) of Streptomyces lividans. J. Gen. Microbiol. 139:677-686.
13. Gilkes, N. R., B. Henrissat, D. G. Kilburn, R. C. Miller, Jr., and R. A. J. Warren. 1991. Domains in microbial β-1,4-glycanases: sequence conservation, function, and enzyme families. Microbiol. Rev. 55:303-315.
14. Goldstein, M. A., M. Takagi, S. Hashlda, O. Shoseyov, R. H. Doi, and I. H. Segel. 1993. Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. J. Bacteriol. 175:5762-5768.
15. He, S. Y., M. Lindeberg, A. K. Chatterjee, and A. Collmer, 1991. Cloned Erwinia chrysanthemi out genes enable Escherichia coli to selectively secrete a diverse family of heterologous proteins to its milieu. Proc. Natl. Acad. Sci. USA 88:1079-1083.
16. Henrissat, B., and A. Bairoch. 1996. Updating the sequence-based classification of glycosyl hydrolases. Biochem. J. 314:695-696.
17. Hoffren, A.-M., T. T. Teeri, and O. Teleman. 1995. Molecular dynamics of fungal cellulose-binding domains: differences in molecular rigidity but a preserved cellulose binding surface. Protein Eng. 8:443-450.
18. Johnson, P., M. Joshi, P. Tomme, D. Kilburn, and L. McIntosh. 1996. Structure of the N-terminal cellulose-binding domain of Cellulomonas fimi Cen C. 2. NMR and ultraviolet spectroscopy. Biochemistry 35:13895-13906.
19. Kamei, K., Y. Yamamura, S. Hara, and T. Ikenaka. 1989. Amino acid sequence of chitinase from Streptomyces erythraeus. J. Biochem. 105:979-985.
20. Kraulis, P., M. Clore, M. Nilges, A. Jones, G. Pettersson, J. Knowles, and A. Gronenborn. 1989. Determination of the three-dimensional solution structure of the C-terminal domain of cellobiohydrolase I from Trichoderma reesei. Biochemistry 28:7241-7257.
21. Kunkel, T. A., K. Bebenek, and J. McClay. 1991. Efficient site-directed mutagenesis using uracil-containing DNA. Methods Enzymol. 204:125-138.
22. Linder, M., M.-L. Mattinen, M. Kontteli, G. Stahlberg, T. Drakenberg, T. Reinikainen, G. Pettersson, and A. Annila. 1995. Identification of functionally important amino acids in the cellulose binding domain of Trichoderma reesei cellobiohydrolase I. Protein Sci. 4:1056-1064.
23. Linder, M., I. Salovuori, L. Ruohonen, and T. T. Teeri. 1996. Characterization of a double cellulose-binding domain. J. Biol. Chem. 271:21268-21272.
24. Mattinen, M. L., M. Kontteli, J. Kerovuo, M. Linder, A. Annila, G. Lindeberg, T. Reinikainen, and T. Drakenberg. 1997. Three-dimensional structures of three engineered cellulose-binding domains of cellobiohydrolase I from Trichoderma reesei. Protein Sci. 6:294-303.
25. Mattinen, M. L., M. Under, T. Drakenberg, and A. Annila. 1998. Solution structure of the cellulose-binding domain of endoglucanase I from Trichoderma reesei and its interaction with cello-oligosaccharides. Eur. J. Biochem. 256:279-286.
26. Morimoto, K., S. Kartta, T. Kimura, K. Sakka, and K. Ohmiya. 1997. Cloning, sequencing, and expression of the gene encoding Clostridium paraputrificum chitinase ChiB and analysis of the functions of novel cadherin-like domains and a chitin-binding domain. J. Bacteriol. 179:7306-7314.
27. Nagy, T., P. Simpson, P. Williamson, G. P. Hazlewood, H. J. Gilbert, and L. Orosz. 1998. All three surface tryptophans in type IIa cellulose binding domains play a pivotal role in binding both soluble and insoluble ligands. FEBS Lett. 429:312-316.
28. Cex) of an exoglucanase from Cellulomonas fimi: production in Escherichia coli and characterisation of the polypeptide. Biotechnol. Bioeng. 42:401-409.
29. Py, B., I. Bortoli-German, J. Haiech, M. Chippaux, and F. Barras. 1991. Cellulase EGZ of Erwinia chrysanthemi: structural organisation and importance of His98 and Glu133 residues for catalysis. Protein Eng. 4:325-333.
30. Relnikainen, T., O. Teleman, and T. T. Teeri. 1995. Effects of pH and high ionic strength on the adsorption and activity of native and mutated cellobiohydrolase I from Trichoderma reesei. Proteins 22:392-403.
31. Sakon, J., D. Irwin, D. B. Wilson, and P. A. Karplus. 1997. Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Nature Struct. Biol. 4:810-818.
32. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
33. Tomme, P., R. A. J. Warren, and N. R. Gilkes. 1995. Cellulose hydrolysis of bacteria and fungi. Adv. Microb. Physiol. 37:1-81.
34. Toone, E. J. 1994. Structure and energetics of protein-carbohydrate complexes. Curr. Opin. Struct. Biol. 4:719-728.
35. Tormo, J., R. Lamed, A. Chirino, E. Morag, E. Bayer, Y. Shoham, and T. Steitz. 1996. Crystal structure of a bacterial family III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J. 15: 5739-5751.
36. Tsujibo, H., H. Orikoshi, K. Shiotani, M. Hayashi, J. Umeda, K. Miyamoto, C. Imada, Y. Okami, and Y. Inamori. 1998. Characterization of chitinase C from a marine bacterium, Alteromonas sp. strain O-7, and its corresponding gene and domain structure. Appl. Environ. Microbiol. 64:472-478.
37. Watanabe, T., Y. Ito, T. Yamada, M. Hashimoto, S. Sekine, and H. Tanaka. 1994. The roles of the C-terminal domain and type III domains of chitinase A1 from Bacillus circulans WL-12 in chitin. J. Bacteriol. 176:4465-4472.
38. Xu, G.-Y., E. Ong, N. Gilkes, D. Kilburn, D. Muhandiram, M. Harris-Brandts, J. Carver, L. Kay, and T. Harvey. 1995. Solution structure of a cellulose-binding domain from Cellulomonas fimi by nuclear magnetic resonance spectroscopy. Biochemistry 34:6993-7009.