Structural stability and endonuclease activity of a PI-SceI GFP-fusion protein

International Journal of Biological Sciences

Volumn 3, Issue 4, 2007, Pages 205-211

  Senejani, Alireza G ^a   Gogarten, J Peter ^a  

^a UNIVERSITY OF CONNECTICUT   (United States)

Author keywords

GFP fusion protein; Homing endonuclease; Intein; PI SceI; Sce VMA

Indexed keywords

ENDONUCLEASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN;

ALLELE; ARTICLE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME SUBSTRATE; INTRON; MOLECULAR RECOGNITION; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN STABILITY; PROTEIN STRUCTURE; WILD TYPE;

EID: 33847032213     PISSN: 14492288     EISSN: None     Source Type: Journal    
DOI: 10.7150/ijbs.3.205     Document Type: Article

References (31)

1. Belfort M and Roberts R.J. Homing endonucleases: keeping the house in order. Nucleic Acids Res, 1997. 25(17): 3379-88.
2. Guhan N and Muniyappa K. Structural and functional characteristics of homing endonucleases. Crit Rev Biochem Mol Biol, 2003. 38(3): 199-248.
3. Stoddard B.L. Homing endonuclease structure and function. Q Rev Biophys, 2005. 38(1): 49-95.
4. Perler F.B. InBase, the Intein Database. Nucleic Acids Res, 2000. 28(1): 344-5.
5. Chevalier B.S and Stoddard B.L. Homing endonucleases: structural and functional insight into the catalysts of intron/intein mobility. Nucleic Acids Res, 2001. 29(18): 3757-74.
6. Gogarten J.P., Senejani A.G., Zhaxybayeva O., Olendzenski L., and Hilario E. Inteins: structure, function, and evolution. Annu Rev Microbiol, 2002. 56: 263-87.
7. Jurica M.S and Stoddard B.L. Homing endonucleases: structure, function and evolution. Cell Mol Life Sci, 1999. 55(10): 1304-26.
8. Bremer M.C., Gimble F.S., Thorner J., and Smith C.L. VDE endonuclease cleaves Saccharomyces cerevisiae genomic DNA at a single site: physical mapping of the VMA1 gene. Nucleic Acids Res, 1992. 20(20): 5484.
9. Duan X., Gimble F.S., and Quiocho F.A. Crystal structure of PI-SceI, a homing endonuclease with protein splicing activity. Cell, 1997. 89(4): 555-64.
10. Moure C.M., Gimble F.S., and Quiocho F.A. Crystal structure of the intein homing endonuclease PI-SceI bound to its recognition sequence. Nat Struct Biol, 2002. 9(10): 764-70.
11. Shimomura O., Johnson F.H., and Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol, 1962. 59: 223-39.
12. Chalfie M., Tu Y., Euskirchen G., Ward W.W., and Prasher D.C. Green fluorescent protein as a marker for gene expression. Science, 1994. 263(5148): 802-5.
13. Prasher D.C., Eckenrode V.K., Ward W.W.,. Prendergast F.G, and Cormier M.J. Primary structure of the Aequorea victoria green-fluorescent protein. Gene, 1992. 111(2): 229-33.
14. Yang F., Moss L.G., and Phillips GNJr. The molecular structure of green fluorescent protein. Nat Biotechnol, 1996. 14(10): 1246-51.
15. Gimble F.S and Thorner J. Purification and characterization of VDE, a site-specific endonuclease from the yeast Saccharomyces cerevisiae. J Biol Chem, 1993. 268(29): 21844-53.
16. Cormack B.P., Valdivia R.H., and Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 1996. 173: 33-8.
17. Studier F.W and Moffatt B.A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol, 1986. 189(1): 113-30.
18. Schagger H and von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem, 1987. 166(2): 368-79.
19. Klabunde T., Sharma S., Telenti A., Jacobs WR Jr., and Sacchettini J.C. Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing [see comments]. Nat Struct Biol, 1998. 5(1): 31-6.
20. Christ F., Steuer S., Thole H., et al. A model for the PI-SceIxDNA complex based on multiple base and phosphate backbone-specific photocross-links. J Mol Biol, 2000. 300(4): 867-75.
21. He Z., Crist M., Yen H., et al. Amino acid residues in both the protein splicing and endonuclease domains of the PI-SceI intein mediate DNA binding. J Biol Chem, 1998. 273(8): 4607-15.
22. Enright A.J., Iliopoulos I., Kyrpides N.C., and Ouzounis C.A. Protein interaction maps for complete genomes based on gene fusion events. Nature, 1999. 402(6757): 86-90.
23. Koonin E.V., Wolf Y.I., and Karev G.P. The structure of the protein universe and genome evolution. Nature, 2002. 420(6912): 218-23.
24. Pasek S., Risler J.L., and Brezellec P. Gene fusion/fission is a major contributor to evolution of multi-domain bacterial proteins. Bioinformatics, 2006. 22(12): 1418-23.
25. Wang J.C. DNA topoisomerases. Annu Rev Biochem, 1996. 65: 635-92.
26. Aroul-Selvam R., Hubbard T., and Sasidharan R. Domain insertions in protein structures. J Mol Biol, 2004. 338(4): 633-41.
27. Noel A.J., Wende W., and Pingoud A. DNA recognition by the homing endonuclease PI-SceI involves a divalent metal ion cofactor-induced conformational change. J Biol Chem, 2004. 279(8): 6794-804.
28. Gimble F.S and Thorner J. Homing of a DNA endonuclease gene by meiotic gene conversion in Saccharomyces cerevisiae. Nature, 1992. 357(6376): 301-6.
29. Hsu M and Berg P. Altering the specificity of restriction endonuclease: effect of replacing Mg2+ with Mn2+. Biochemistry, 1978. 17(1): 131-38.
30. Zhang A., Gonzalez S.M., Cantor E.J., and Chong S. Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene, 2001. 275(2): 241-52.
31. Gogarten J.P and Hilario E. Inteins, introns, and homing endonucleases: recent revelations about the life cycle of parasitic genetic elements. BMC Evol Biol, 2006. 6: 94.