Towards an understanding of apoptosis detection by SYTO dyes

Cytometry Part A

Volumn 71, Issue 2, 2007, Pages 61-72

  Wlodkowic, Donald ^a   Skommer, Joanna ^a   Pelkonen, Jukka ^a,b  

^a UNIVERSITY OF EASTERN FINLAND   (Finland)

^b KUOPIO UNIVERSITY HOSPITAL   (Finland)

Author keywords

m dissipation; Apoptosis; Multiparameter flow cytometry; Oncosis; SYTO

Indexed keywords

2 AMINO 6 BROMO 4 (1 CYANO 2 ETHOXY 2 OXOETHYL) 4H CHROMENE 3 CARBOXYLIC ACID ETHYL ESTER; BENZYLOXYCARBONYLVALYLALANYLASPARTYL FLUOROMETHYL KETONE; CARBONYL CYANIDE 4 (TRIFLUOROMETHOXY)PHENYLHYDRAZONE; CASPASE INHIBITOR; DYE; HOE 33342; SYTO 16; SYTO GREEN; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL DEATH; CONTROLLED STUDY; DISEASE MARKER; FLOW CYTOMETRY; FLUORESCENCE; FOLLICULAR LYMPHOMA; HUMAN; HUMAN CELL; LYMPHOCYTE SUBPOPULATION; PRIORITY JOURNAL; RELIABILITY; STAINING;

APOPTOSIS; CASPASES; CELL LINE, TUMOR; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE;

EID: 33846908541     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20366     Document Type: Article

References (29)

1. Darzynkiewicz Z, Juan G, Li X, Gorczyca W, Murakami T, Traganos F. Cytometry in cell necrobiology: Analysis of apoptosis and accidental cell death (necrosis). Cytometry 1997;27:1-20.
2. Bedner E, Li X, Gorczyca W, Melamed MR, Darzynkiewicz Z. Analysis of apoptosis by laser scanning cytometry. Cytometry 1999;35:181-195.
3. Lecoeur H, de Oliveira-Pinto LM, Gougeon ML. Multiparametric flow cytometric analysis of biochemical and functional events associated with apoptosis and oncosis using the 7-aminoactinomycin D assay. J Immunol Methods 2002;265:81-96.
4. Barbier M, Gray BD, Muirhead KA, Ronot X, Boutonnat J. A flow cytometric assay for simultaneous assessment of drug efflux, proliferation, and apoptosis. Cytometry B Clin Cytom 2004;59:46-53.
5. Marin L, Minguela A, Torio A, Moya-Quiles MR, Muro M, Montes-Ares O, Parrado A, Alvarez-Lopez DM, Garcia-Alonso AM. Flow cytometric quantification of apoptosis and proliferation in mixed lymphocyte culture. Cytometry 2003;51:107-118.
6. Pozarowski P, Huang X, Halicka DH, Lee B, Johnson G, Darzynkiewicz Z. Interactions of fluorochrome-labeled caspase inhibitors with apoptotic cells: A caution in data interpretation. Cytometry 2003;55:50-60.
7. Rasola A, Geuna M. A flow cytometry assay simultaneously detects independent apoptotic parameters. Cytometry 2001;45:151-157.
8. Halicka HD, Seiter K, Feldman EJ, Traganos F, Mittelman A, Ahmed T, Darzynkiewicz Z. Cell cycle specificity of apoptosis during treatment of leukaemias. Apoptosis 1997;2:25-39.
9. Zhivotovsky B. Apoptosis, necrosis and between. Cell Cycle 2004;3:64-66.
10. Leist M, Jaattela M. Four deaths and a funeral: From caspases to alternative mechanisms. Nat Rev Mol Cell Biol 2001;2:589-598.
11. Frey T. Nucleic acid dyes for detection of apoptosis in live cells. Cytometry 1995;21:265-274.
12. Eray M, Matto M, Kaartinen M, Andersson L, Pelkonen J. Flow cytometric analysis of apoptotic subpopulations with a combination of annexin V-FITC, propidium iodide, and SYTO 17. Cytometry 2001;43:134-142.
13. Poot M, Gibson LL, Singer VL. Detection of apoptosis in live cells by MitoTracker red CMXRos and SYTO dye flow cytometry. Cytometry 1997;27:358-364.
14. Schuurhuis GJ, Muijen MM, Oberink JW, de Boer F, Ossenkoppele GJ, Broxterman HJ. Large populations of non-clonogenic early apoptotic CD34-positive cells are present in frozen-thawed peripheral blood stem cell transplants. Bone Marrow Transplant 2001;27:487-498.
15. Tong D, Poot M, Hu D, Oda D. 5-Fluorouracil-induced apoptosis in cultured oral cancer cells. Oral Oncol 2000;36:236-241.
16. Serafeim A, Holder MJ, Grafton G, Chamba A, Drayson MT, Luong QT, Bunce CM, Gregory CD, Barnes NM, Gordon J. Selective serotonin reuptake inhibitors directly signal for apoptosis in biopsy-like Burkitt lymphoma cells. Blood 2003;101:3212-3219.
17. van Zandvoort MA, de Grauw CJ, Gerritsen HC, Broers JL, oude Egbrink MG, Ramaekers FC, Slaaf DW. Discrimination of DNA and RNA in cells by a vital fluorescent probe: Lifetime imaging of SYTO13 in healthy and apoptotic cells. Cytometry 2002;47:226-235.
18. Mundy WR, Freudenrich TM. Apoptosis of cerebellar granule cells induced by organotin compounds found in drinking water: Involvement of MAP kinases. Neurotoxicology 2006;27:71-81.
19. Broxterman HJ, Schuurhuis GJ, Lankelma J, Oberink JW, Eekman CA, Claessen AM, Hoekman K, Poot M, Pinedo HM. Highly sensitive and specific detection of P-glycoprotein function for haematological and solid tumour cells using a novel nucleic acid stain. Br J Cancer 1997;76:1029-1034.
20. Andreescu S, Sadik OA, McGee DW. Effect of natural and synthetic estrogens on a549 lung cancer cells: Correlation of chemical structures with cytotoxic effects. Chem Res Toxicol 2005;18:466-474.
21. Holmstrom TH, Chow SC, Elo I, Coffey ET, Orrenius S, Sistonen L, Eriksson JE. Suppression of Fas/APO-1-mediated apoptosis by mitogen-activated kinase signaling. J Immunol 1998;160:2626-2636.
22. Knowles RB, Sabry JH, Martone ME, Deerinck TJ, Ellisman MH, Bassell GJ, Kosik KS. Translocation of RNA granules in living neurons. J Neurosci 1996;16:7812-7820.
23. van der Pol MA, Broxterman HJ, Westra G, Ossenkoppele GJ, Schuurhuis GJ. Novel multiparameter flow cytometry assay using Syto16 for the simultaneous detection of early apoptosis and apoptosis-corrected P-glycoprotein function in clinical samples. Cytometry B Clin Cytom 2003;55:14-21.
24. Sparrow RL, Tippett E. Discrimination of live and early apoptotic mononuclear cells by the fluorescent SYTO 16 vital dye. J Immunol Methods 2005;305:173-187.
25. Skommer J, Wlodkowic D, Matto M, Eray M, Pelkonen J. HA14-1, a small molecule Bcl-2 antagonist, induces apoptosis and modulates action of selected anticancer drugs in follicular lymphoma B cells. Leuk Res 2006;30:322-331.
26. Skommer J, Wlodkowic D, Pelkonen J. Cellular foundation of curcumin-induced apoptosis in follicular lymphoma cell lines. Exp Hematol 2006;34:463-474.
27. Wlodkowic D, Skommer J, Pelkonen J. Multiparametric analysis of HA14-1-induced apoptosis in follicular lymphoma cells. Leuk Res 2006;30:1187-1192.
28. 2+-dependent proteases in ischemic neuronal death: A conserved "calpain-cathepsin cascade" from nematodes to primates. Cell Calcium 2004;36:285-293.
29. Kroemer G, Martin SJ. Caspase-independent cell death. Nat Med 2005;11:725-730.