Highly sensitive and specific detection of P-glycoprotein function for haematological and solid tumour cells using a novel nucleic acid stain

British Journal of Cancer

Volumn 76, Issue 8, 1997, Pages 1029-1034

  Broxterman, H J ^a,c   Schuurhuis, G J ^a   Lankelma, J ^a   Oberink, J W ^a   Eekman, C A ^a   Claessen, Ame ^a   Hoekman, K ^a   Poot, M ^b   Pinedo, H M ^a  

^a ACADEMIC MEDICAL CENTER   (Netherlands)

^b MOLECULAR PROBES INC   (United States)

^c VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

CD34+; DNA binding; Fluorescence detection; P glycoprotein; PSC 833; Sarcoma; SYTO16

Indexed keywords

CD34 ANTIGEN; DAUNORUBICIN; DYE; FORMALDEHYDE; GLYCOPROTEIN P; NUCLEIC ACID; RHODAMINE 123; VALSPODAR;

ACUTE GRANULOCYTIC LEUKEMIA; ARTICLE; CELL NUCLEUS; CONTROLLED STUDY; DRUG RESISTANCE; FLOW CYTOMETRY; FLUORESCENCE; HEMATOLOGIC DISEASE; HUMAN; HUMAN CELL; LEIOMYOSARCOMA; PRIORITY JOURNAL; SARCOMA CELL; SCANNING ELECTRON MICROSCOPY; SOLID TUMOR; TREATMENT FAILURE; TUMOR CELL;

EID: 0030769699     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/bjc.1997.503     Document Type: Article

References (23)

1. Beck WT, Grogan TM, Willman CL, Cordon-Cardo C, Parham DM, Kuttesch JF, Andreeff M, Bates S, Boyett JM, Brophy N, Broxterman HJ, Chan HSL, Dalton WS, Dietel M, Fojo AT, Gascoyne R, Head D, Houghton PJ, Srivastava DK, Lehnert M, Paietta E, Pavelic ZP, Rimzsa L, Roninson IB, Sikic BI, Twentyman PR, Warnke R and Weinstein R (1996) Methods to detect P-glycoprotein-associated multidrug resistance: consensus recommendations. Cancer Res 56: 3010-3020
2. Broxterman HJ, Schuurhuis GJ, Lankelma J, Baak JPA and Pinedo HM (1990) Towards functional screening for multidrug resistant cells in human malignancies. In Pezcoller Foundation Symposium, Vol. 1. Drug Resistance: Mechanism and Reversal, Mihich E, (ed.), pp. 309-319. John Libbey CIC: Rome
3. Broxterman HJ, Feller N, Kuiper CM, Boven E, Versantvoort CHM, Teerlink T, Pinedo, HM and Lankelma J (1995) Correlation between functional and molecular analysis of mdr1 P-glycoprotein in human solid tumor xenografts. Int J Cancer 61: 880-886
4. Broxterman HJ, Lankelma J and Pinedo HM (1996a) How to probe clinical tumour samples for P-glycoprotein and multidrug resistance-associated protein. Eur J Cancer 32A: 1024-1033
5. Broxterman HJ, Sonneveld P, Feller N, Ossenkoppele GJ, Währer DCR, Eckman CA, Schoester M, Lankelma J, Pinedo HM, Löwenberg B and Schuurhuis GJ (1996b) Quality control of multidrug resistance assays in adult acute leukemia: correlation between assays for P-glycoprotein expression and activity. Blood 86: 4809-4816
6. Broxterman HJ, Lankelma J, Pinedo HM, Eckman CA, Währer DCR, Ossenkoppele GJ and Schuurhuis GJ (1997) Theoretical and practical considerations for the measurement of P-glycoprotein function in acute myeloid leukemia. Leukemia 11: (in press)
7. Chaudhary PM and Roninson IB (1991) Expression and activity of P-glycoprotein, a multidrug efflux pump, in human hematopoietic stem cells. Cell 66: 85-94
8. Dairkee SH, Deng G, Stampfer MR, Waldman FM and Smith HS (1995). Selective cell culture of primary breast carcinoma. Cancer Res 55: 2516-2519
9. Feller N, Broxterman HJ, Währer DCR and Pinedo HM (1995a) ATP-dependent efflux of calcein by the multidrug resistance protein (MRP): no inhibition by intracellular glutathione depletion. FEBS Lett 368: 385-388
10. Feller N, Kuiper CM, Lankelma J, Ruhdal JK, Scheper RJ, Pinedo HM and Broxterman HJ (1995b) Functional detection of MDR1/P170 and MRP/P190-mediated multidrug resistance in tumour cells by flow cytometry. Br J Cancer 72: 543-549
11. Ino T, Miyazaki H, Isogai M, Nomura I, Tsuzuki M, Tsuruo T, Ezaki K and Hirano M (1994) Expression of P-glycoprotein in de novo acute myelogenous leukemia at initial diagnosis: results of molecular and functional assays, and correlation with treatment outcome. Leukemia 8: 1492-1497
12. Juliano, RL and Ling V (1976) A surface glycoprotein modulating drug permeability in Chinese hamster ovary cell mutants. Biochem Biophys Acta 455: 152-162
13. Kartner N, Evernden-Porelle D, Bradley G and Ling V (1985) Detection of P-glycoprotein in multidrug-resistant cell lines by monoclonal antibodies. Nature 316: 820-823
14. Kunikane H, Zalupski MM, Kukuruga M, Lucas DR, Ryan JR, Krishan A and Vaitkevicius V (1995) P-glycoprotein expression and drug efflux in soft tissue sarcoma. Proc Am Assoc Cancer Res 36: 219
15. +/efflux cases. Blood 86: 2329-2342
16. Ljung B-M, Mayall B, Lottich C, Boyer C, Sylvester SS, Leight GS, Siegler HF and Smith HS (1989) Cell dissociation techniques in human breast cancer - variations in tumor cell viability and DNA ploidy. Breast Cancer Res Treat 13: 153-159
17. Marie J-P, Faussat-Suberville A-M, Zhou D and Zittoun R (1992) Daunorubicin uptake by leukemic cells: correlations with treatment outcome and mdr1 expression. Leukemia 7: 825-831
18. Neyfakh AA, Serpinskaya AS, Chervonsky AV, Apasov SG and Kazarov AR (1989) Multidrug resistance phenotype of a subpopulation of T-lymphocytes without drug selection. Exp Cell Res 185: 496-505
19. Noonan KE, Beck C, Holzmayer TA, Chin JE, Wunder JS, Andrulis IL, Gazdar AF, Willman CL, Griffith B, Von Hoff DD and Roninson IB (1990) Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction. Proc Natl Acad Sci USA 87: 7160-7164
20. Ross DD, Wooten PJ, Sridhara R, Ordóñez JV, Lee EJ and Schiffer CA (1993) Enhancement of daunorubicin accumulation, retention, and cytotoxicity by verapamil or cyclosporin A in blast cells from patients with previously untreated acute myeloid leukemia. Blood 82: 1288-1299
21. Scheper RJ, Bulte JWM, Brakkee JGP, Quak JJ, Van Der Schoot E, Balm AJM, Meijer CJLM, Broxterman HJ, Kuiper CM, Lankelma J and Pinedo HM (1988) Monoclonal antibody JSB-1 detects a highly conserved epitope on the P-glycoprotein associated with multidrug resistance. Int J Cancer 42: 389-394
22. Spoelstra EC, Westerhoff HV, Dekker H and Lankelma J (1992) Kinetics of daunorubicin transport by P-glycoprotein of intact cancer cells. Eur J Biochem 207: 567-579
23. Willingham MC, Cornwell MM, Cardarelli CO, Gottesman MM and Pastan I (1986) Single cell analysis of daunomycin uptake and efflux in multidrug-resistant and-sensitive KB cells: effects of verapamil and other drugs. Cancer Res 46: 5941-5946