Flow Cytometric Quantification of Apoptosis and Proliferation in Mixed Lymphocyte Culture

Cytometry Part A

Volumn 51, Issue 2, 2003, Pages 107-118

  Marín, Luis ^a   Minguela, Alfredo ^a   Torío, Alberto ^a   Moya Quiles, María R ^a   Muro, Manuel ^a   Montes Ares, Olga ^a   Parrado, Antonio ^a   Alvarez López, D María Rocío ^a   García Alonso, Ana M ^a  

^a HOSPITAL UNIVERSITARIO VIRGEN DE LA ARRIXACA   (Spain)

Author keywords

7 aminoactinomycin D; Apoptosis; Carboxy fluorescein succinimidyl ester; Mixed lymphocyte cultures; Proliferation; Transplantation

Indexed keywords

CARBOXYFLUORESCEIN DIACETATE SUCCINIMIDYL ESTER; DACTINOMYCIN; MONOCLONAL ANTIBODY; THYMIDINE DERIVATIVE;

APOPTOSIS; ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IN VITRO STUDY; IRRADIATION; LYMPHOCYTE CULTURE; LYMPHOCYTE PROLIFERATION; MONONUCLEAR CELL; NORMAL HUMAN; PHENOTYPE; PRIORITY JOURNAL; RADIOACTIVITY; RELIABILITY; TRANSPLANTATION;

EID: 0344716645     PISSN: 15524922     EISSN: None     Source Type: Journal    
DOI: 10.1002/cyto.a.10007     Document Type: Article

References (26)

1. Cohen JJ. Apoptosis Mechanisms of life and death in the immune system. J Allergy Clin Immunol 1999;103:548-554.
2. Mogil RJ, Radvanyi L, Gonzalez-Quintial R, Miller R, Mills G, Theofilopoulos AN, Green R. Fas (CD95) participates in peripheral T cell deletion and associated apoptosis in vivo. Int Immunol 1995;7:1451-1458.
3. Lowin B, Mattman C, Hahne M, Tschopp J. Comparison of Fas(Apo-1/CD95) -and perforin-mediated cytotoxicity in primary T lymphocytes. Int Immunol 1996;8:57-63.
4. Kabelitz D. Apoptosis, graft rejection, and transplantation tolerance. Transplantation 1998;65:869-875.
5. Schmid I, Uittenbogaart CH, Giorgi JV. Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 1994;15:12-20.
6. Lecoeur H, Ledru E, Prévost MC, Gougeon ML. Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorimetric staining methods. J Immunol Methods 1997;209:111-123.
7. Schmid I, Uittenbogaart CH, Keld B, Giorgi JV. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J Immunol Methods 1994;170:145-157.
8. Bach FH, Voynow NK. One-way stimulation in mixed leukocyte cultures. Science 1966;153:545-547.
9. Mickelson EM, Longton G, Anasetti C, Petersdorf E, Martin P, Guthrie LA, Hansen JA. Evaluation of mixed lymphocyte culture (MLC) assay as a method for selecting unrelated donors for marrow transplantation. Tissue Antigens 1996;47:27-36.
10. Matzinger P. The JAM test. A simple assay for DNA fragmentation and cell death. J Immunol Methods 1991;145:185-192.
11. Sato T, Deiwick A, Raddatz G, Koyama K, Schiltt HJ. Interactions of allogenic human mononuclear cells in two-way mixed leucocyte culture (MLC): influence of cells numbers subpopulations and cyclosporin. Clin Exp Immunol 1999;115:301-308.
12. Weston SA, Parish CR. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J Immunol Methods 1990;133:87-97.
13. Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J Immunol Methods 1994;171:131-137.
14. Flieger D, Gruber R, Schlimok G, Reiter C, Pantel K, Riethmüller G. A novel non-radioactive cellular cytotoxicity test based on the differential assessment of living and killed target and effector cells. J Immunol Methods 1995;180:1-13.
15. Reap EA, Roof K, Maynor K, Borrero M, Booker J, Cohen P. Radiation and stress-induced apoptosis: a role for Fas/Fas ligand interactions. Proc Natl Acad Sci USA 1997;94:5750-5755.
16. Philippé J, Louagie H, Thierens H, Vral A, Cornelissen M, De Ridder L. Quantification of apoptosis in lymphocyte subsets and effect of apoptosis on apparent expression of membrane antigens. Cytometry 1997;29:242-249.
17. Popma SH, Krasinskas AM, Mclean AD, Szeto WY, Kreisel D, Moore JS, Rosengard BR. Immune monitoring in xenotransplantation: the multiparameter flow cytometric lymphocyte culture assay. Cytometry 2000;42:277-283.
18. Lecoeur H, Février M, Garcia S, Rivière Y, Gougeon ML. A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicity. J Immunol Methods 2001;253:177-187.
19. Slezak SE, Horan PK. Fluorescent in vivo tracking of hematopoietic cells. Part I. Technical considerations. Blood 1989;74:2172-2177.
20. Terstappen LWMM, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigen on five lineages of mature peripheral blood cells. J Leukoc Biol 1990;48:138-148.
21. Brando B, Barnett D, Janossy G, Mandy F, Autran B, Rothe G, Scarpati B, D'Avanzo G, D'Hautcourt JL, Lenkei R, Schmitz G, Kunkl A, Chianese R, Papa S, Gratama JW. Cytofluorimetric methods for assessing absolute numbers of cell subsets in blood. Cytometry 2000; 42:327-346.
22. Darzynkiewicz Z, Bedner E, Traganos F, Murakami T. Critical aspects in the analysis of apoptosis and necrosis. Hum Cell 1998;11:3-12.
23. O'Flaherty E, Ali S, Pettit SJ, Kirby JA. Examination of the sensitivity of T cells to Fas ligation. Induction of allospecific apoptosis. Transplantation 1998;66:1067-1073.
24. Dubey DP, Yunis I, Yunis EJ: Cellular typing. Mixed lymphocyte response and cell-mediated lympholysis. In: Rose NR, Friedman H, Fahey JL, editors. Manual of clinical laboratory immunology. 3th ed. Washington, DC: American Society for Microbiology; 1986. p 847-858
25. Sharland A, Yan Y, Wang C, Bowen DG, Sun J, Sheil AGR, McCaughan GW, Bishop GA. Evidence that apoptosis of activated T cells occurs in spontaneous tolerance of liver allografts and is blocked by manipulations which break tolerance. Transplantation 1999;68:1736-1745.
26. Genestier L, Bonnfeoy-Berard N, Revillard JP. Apoptosis of activated peripheral T cells. Transplant Proc 1999;31(suppl 1/2A):33S-38S.